BMRB Entry 5996

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5996

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Title:
Temperature Dependent Spectral Density Analysis Applied to Monitoring Backbone Dynamics of Major Urinary Protein-I Complexed with the Pheromone 2-sec-Butyl-4,5-dihyrothiazole
Deposition date:
2003-11-05
Original release date:
2004-04-07
Authors:
Krizova, Hana; Zidek, Lukas; Stone, Martin; Novotny, Milos; Sklenar, Vladimir
Citation:

Citation: Krizova, Hana; Zidek, Lukas; Stone, Martin; Novotny, Milos; Sklenar, Vladimir. "Temperature Dependent Spectral Density Analysis Applied to Monitoring Backbone Dynamics of Major Urinary Protein-I Complexed with the Pheromone 2-sec-butyl-4,5-dihyrothiazole"  J. Biomol. NMR 28, 369-384 (2004).
PubMed: 14872128

Assembly members:

Assembly members:
Major Urinary Protein-I, polymer, 162 residues, Formula weight is not available
2-[(1S)-1-methylpropyl]-4,5-dihydro-1,3-thiazole, non-polymer, 143.250 Da.

Natural source:

Natural source:   Common Name: House mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Major Urinary Protein-I: EEASSTGRNFNVEKINGEWH TIILASDKREKIEDNGNFRL FLEQIHVLENSLVLKFHTVR DEECSELSMVADKTEKAGEY SVTYDGFNTFTIPKTDYDNF LMAHLINEKDGETFQLMGLY GREPDLSSDIKERFAQLCEK HGILRENIIDLSNANRCLQA RE

Data typeCount
15N chemical shifts159
1H chemical shifts159
T1 relaxation values979
T2 relaxation values979
heteronuclear NOE values979

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MUP-I1
22-sec-butyl-4,5-dihydrothiazole2

Entities:

Entity 1, MUP-I 162 residues - Formula weight is not available

1   GLUGLUALASERSERTHRGLYARGASNPHE
2   ASNVALGLULYSILEASNGLYGLUTRPHIS
3   THRILEILELEUALASERASPLYSARGGLU
4   LYSILEGLUASPASNGLYASNPHEARGLEU
5   PHELEUGLUGLNILEHISVALLEUGLUASN
6   SERLEUVALLEULYSPHEHISTHRVALARG
7   ASPGLUGLUCYSSERGLULEUSERMETVAL
8   ALAASPLYSTHRGLULYSALAGLYGLUTYR
9   SERVALTHRTYRASPGLYPHEASNTHRPHE
10   THRILEPROLYSTHRASPTYRASPASNPHE
11   LEUMETALAHISLEUILEASNGLULYSASP
12   GLYGLUTHRPHEGLNLEUMETGLYLEUTYR
13   GLYARGGLUPROASPLEUSERSERASPILE
14   LYSGLUARGPHEALAGLNLEUCYSGLULYS
15   HISGLYILELEUARGGLUASNILEILEASP
16   LEUSERASNALAASNARGCYSLEUGLNALA
17   ARGGLU

Entity 2, 2-sec-butyl-4,5-dihydrothiazole - C7 H13 N S - 143.250 Da.

1   ZBT

Samples:

sample_1: Major Urinary Protein-I, [U-95% 15N], 1.8 mM; 2-(SEC-BUTYL)-4,5-DIHYDROTHIAZOLE 1.8 mM

283K: pH: 6.3; temperature: 283 K

288K: pH: 6.3; temperature: 288 K

293K: pH: 6.3; temperature: 293 K

298K: pH: 6.3; temperature: 298 K

303K: pH: 6.3; temperature: 303 K

308K: pH: 6.3; temperature: 308 K

291K: pH: 6.3; temperature: 291 K

297K: pH: 6.3; temperature: 297 K

302K: pH: 6.3; temperature: 302 K

Experiments:

NameSampleSample stateSample conditions
15N T1sample_1not availablenot available
15N T2sample_1not availablenot available
{1H}-15N NOEsample_1not availablenot available

Software:

NMRPipe -

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

BMRB 17447 4340 5995
PDB
DBJ BAB28753 BAB29093
EMBL CAA26953 CAA27227 CAA27729 CAC34259 CAQ11104
GB AAA39764 AAA39765 AAA39767 AAA39768 AAB47130
REF NP_001039015 NP_001116119 NP_001128116 NP_001128147 NP_001128148
SP B5X0G2 P02762 P04938 P11588 P11589
TPG DAA06299 DAA06300 DAA06301 DAA06302 DAA06303
AlphaFold B5X0G2 P02762 P04938 P11588 P11589

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks