BMRB Entry 17447

Name: 1H, 15N and 13C resonance assignment of a common Major Urinary Protein of the mouse
Published: 2012-08-30

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PDB ID: 2lb6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17447
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C resonance assignment of a common Major Urinary Protein of the mouse

Deposition date:

2011-02-07

Original release date:

2012-08-30

Authors:

Phelan, Marie; McLean, Lynn; Simpson, Deborah; Hurst, Jane; Beynon, Robert; Lian, Lu-Yun

Citation:

Citation: Phelan, Marie; McLean, Lynn; Simpson, Deborah; Hurst, Jane; Beynon, Robert; Lian, Lu-Yun. "1H, 15N and 13C resonance assignment of darcin, a mouse major urinary protein." Biomol NMR Assign 4, 239-241 (2010).
PubMed: 20703836

Assembly members:

Assembly members:
MUP11, polymer, 176 residues, 20336.5 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MUP11: MGSSHHHHHHIEGREEASST GRNFNVEKINGEWHTIILAS DKREKIEDNGNFRLFLEQIH VLENSLVLKFHTVRDEECSE LSMVADKTEKAGEYSVTYDG FNTFTIPKTDYDNFLMAHLI NEKDGETFQLMGLYGREPDL SSDIKERFAQLCEEHGILRE NIIDLSNANRCLQARE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	714
15N chemical shifts	170
1H chemical shifts	1074

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MUP11	1

Entities:

Entity 1, MUP11 176 residues - 20336.5 Da.

residues 20 to 181 correspond to the native MUP residues 6 to 19 correspond to the cloning and purification tag

1

MET

GLY

SER

HIS

2

ILE

GLU

GLY

ARG

GLU

ALA

SER

THR

3

GLY

ARG

ASN

PHE

ASN

VAL

GLU

LYS

ILE

ASN

4

GLY

GLU

TRP

HIS

THR

ILE

LEU

ALA

SER

5

ASP

LYS

ARG

GLU

LYS

ILE

GLU

ASP

ASN

GLY

6

ASN

PHE

ARG

LEU

PHE

LEU

GLU

GLN

ILE

HIS

7

VAL

LEU

GLU

ASN

SER

LEU

VAL

LEU

LYS

PHE

8

HIS

THR

VAL

ARG

ASP

GLU

CYS

SER

GLU

9

LEU

SER

MET

VAL

ALA

ASP

LYS

THR

GLU

LYS

10

ALA

GLY

GLU

TYR

SER

VAL

THR

TYR

ASP

GLY

11

PHE

ASN

THR

PHE

THR

ILE

PRO

LYS

THR

ASP

12

TYR

ASP

ASN

PHE

LEU

MET

ALA

HIS

LEU

ILE

13

ASN

GLU

LYS

ASP

GLY

GLU

THR

PHE

GLN

LEU

14

MET

GLY

LEU

TYR

GLY

ARG

GLU

PRO

ASP

LEU

15

SER

ASP

ILE

LYS

GLU

ARG

PHE

ALA

GLN

16

LEU

CYS

GLU

HIS

GLY

ILE

LEU

ARG

GLU

17

ASN

ILE

ASP

LEU

SER

ASN

ALA

ASN

ARG

18

CYS

LEU

GLN

ALA

ARG

GLU

Samples:

sample_1: MUP11, [U-98% 13C; U-98% 15N], 1 mM; potassium phosphate 25 mM; sodium azide 0.2%; H2O 90%; D2O 10%

sample_2: MUP11, [U-98% 13C; U-98% 15N], 1 mM; potassium phosphate 25 mM; sodium azide 0.2%; D2O 100%

sample_conditions_1: ionic strength: 25 mM; pH: 6.8; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHANH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
2D (HB)CB(CGCD)HD	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC (aromatic)	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

ANALYSIS v2.1.5, CCPN - chemical shift assignment, data analysis

DANGLE v1.1, CCPN - Secondary structure calculation

AZARA, Boucher - processing

TOPSPIN v2.1, Bruker Biospin - collection, refinement

CSI, David Wishart, Brian Sykes - Secondary structure calculation

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz

UNP	Q9CXU6
MGI	3709617
BMRB	4340
PDB	1DF3 1I04 1I05 1I06 1JV4 1MUP 2LB6
DBJ	BAB28753 BAB29093
EMBL	CAA26953 CAA27227 CAA27729 CAC34259 CAQ11104
GB	AAA39764 AAA39765 AAA39767 AAA39768 AAB47130
REF	NP_001039015 NP_001116119 NP_001128116 NP_001128146 NP_001128147
SP	B5X0G2 P02762 P04938 P11588 P11589
TPG	DAA06299 DAA06300 DAA06301 DAA06302 DAA06303
AlphaFold	Q9CXU6 B5X0G2 P02762 P04938 P11588 P11589