BMRB Entry 5951
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5951
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Citation: John, Michael; Patard, Louis; Wendeler, Michaela; Sandhoff, Konrad; Kessler, Horst. "1H and 15N chemical shift assignment and secondary structure of human saposin C" The BMRB entry is the only known published source for the data..
Assembly members:
saposin C, polymer, 87 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
saposin C: SDVYCEVCEFLVKEVTKLID
NNKTEKEILDAFDKMCSKLP
KSLSEECQEVVDTYGSSILS
ILLEEVSPELVCSMLHLCSG
RHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 75 |
| 1H chemical shifts | 521 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SAP-C | 1 |
Entities:
Entity 1, SAP-C 87 residues - Formula weight is not available
| 1 | SER | ASP | VAL | TYR | CYS | GLU | VAL | CYS | GLU | PHE | ||||
| 2 | LEU | VAL | LYS | GLU | VAL | THR | LYS | LEU | ILE | ASP | ||||
| 3 | ASN | ASN | LYS | THR | GLU | LYS | GLU | ILE | LEU | ASP | ||||
| 4 | ALA | PHE | ASP | LYS | MET | CYS | SER | LYS | LEU | PRO | ||||
| 5 | LYS | SER | LEU | SER | GLU | GLU | CYS | GLN | GLU | VAL | ||||
| 6 | VAL | ASP | THR | TYR | GLY | SER | SER | ILE | LEU | SER | ||||
| 7 | ILE | LEU | LEU | GLU | GLU | VAL | SER | PRO | GLU | LEU | ||||
| 8 | VAL | CYS | SER | MET | LEU | HIS | LEU | CYS | SER | GLY | ||||
| 9 | ARG | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: saposin C, [U-15N], 1.4 mM; sodium phosphate 50 mM
sample_2: saposin C, [U-15N], 1.4 mM; sodium acetate 50 mM
pH7: pH: 7.0; temperature: 310 K
pH4: pH: 4.0; temperature: 290 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| HNHA | not available | not available | not available |
| HNHB | not available | not available | not available |
| 15N HSQC-NOESY-HSQC | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DMX 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks