BMRB Entry 5465

Name: NMR solution structure of human Saposin C
Published: 2004-03-15

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PDB ID: 1m12
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5465
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR solution structure of human Saposin C

Deposition date:

2002-07-16

Original release date:

2004-03-15

Authors:

de Alba, E.; Weiler, S.; Tjandra, N.

Citation:

Citation: de Alba, E.; Weiler, S.; Tjandra, N.. "Solution structure of human saposin C: pH-dependent interaction with phospholipid vesicles." Biochemistry 42, 14729-14740 (2003).
PubMed: 14674747

Assembly members:

Assembly members:
SAPOSIN C, polymer, 84 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SAPOSIN C: SDVYCEVCEFLVKEVTKLID NNKTEKEILDAFDKMCSKLP KSLSEECQEVVDTYGSSILS ILLEEVSPELVCSMLHLCSG LVPR

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	380
15N chemical shifts	82
1H chemical shifts	610

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SAPOSIN C	1

Entities:

Entity 1, SAPOSIN C 84 residues - Formula weight is not available

1

SER

ASP

VAL

TYR

CYS

GLU

VAL

CYS

GLU

PHE

2

LEU

VAL

LYS

GLU

VAL

THR

LYS

LEU

ILE

ASP

3

ASN

LYS

THR

GLU

LYS

GLU

ILE

LEU

ASP

4

ALA

PHE

ASP

LYS

MET

CYS

SER

LYS

LEU

PRO

5

LYS

SER

LEU

SER

GLU

CYS

GLN

GLU

VAL

6

VAL

ASP

THR

TYR

GLY

SER

ILE

LEU

SER

7

ILE

LEU

GLU

VAL

SER

PRO

GLU

LEU

8

VAL

CYS

SER

MET

LEU

HIS

LEU

CYS

SER

GLY

9

LEU

VAL

PRO

ARG

Samples:

sample_1: SAPOSIN C, [U-15N], 1 mM; NaCl10 – 20 mM; H20 90%; D20 10%

sample_2: SAPOSIN C, [U-13C; U-15N], 1 mM; NaCl10 – 20 mM; H20 90%; D20 10%

sample_3: SAPOSIN C, [U-13C; U-15N], 1 mM; NaCl10 – 20 mM; D20 100%

sample_cond_1: ionic strength: 15 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	not available	not available	not available
4D 13C/15N-separated NOESY	not available	not available	not available
4D 13C-separated NOESY	not available	not available	not available
3D 13C-separated TOCSY	not available	not available	not available
CBCACONH	not available	not available	not available
HNCACB	not available	not available	not available
experiments for residual dipolar coupling measurements	not available	not available	not available

Software:

xwinnmr v2.5 - collection

NMRPipe v2.1 - processing

PIPP v4.2.8 - data analysis

X-PLOR v3.840 - refinement

NMR spectrometers:

Bruker DRX 800 MHz
Bruker DMX 600 MHz