BMRB Entry 5839
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5839
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Hall, Jennifer; Fushman, David. "Characterization of the Overall and Local Dynamics of a Protein with
Intermediate Rotational Anisotropy: Differentiating Between Conformational
Exchange and Anisotropic Diffusion in the B3 Domain of Protein G " J. Biomol. NMR 27, 261-275 (2003).
PubMed: 12975584
Assembly members:
B3 domain of Streptococcal protein G, polymer, 56 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 1301 Superkingdom: Eubacteria Kingdom: not available Genus/species: Streptococcus not available
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
B3 domain of Streptococcal protein G: MQYKLVINGKTLKGETTTKA
VDAETAEKAFKQYANDNGVD
GVWTYDDATKTFTVTE
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
- heteronucl_NOEs
| Data type | Count |
| T1 relaxation values | 51 |
| T2 relaxation values | 51 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GB3 subunit 1 | 1 |
Entities:
Entity 1, GB3 subunit 1 56 residues - Formula weight is not available
| 1 | MET | GLN | TYR | LYS | LEU | VAL | ILE | ASN | GLY | LYS | ||||
| 2 | THR | LEU | LYS | GLY | GLU | THR | THR | THR | LYS | ALA | ||||
| 3 | VAL | ASP | ALA | GLU | THR | ALA | GLU | LYS | ALA | PHE | ||||
| 4 | LYS | GLN | TYR | ALA | ASN | ASP | ASN | GLY | VAL | ASP | ||||
| 5 | GLY | VAL | TRP | THR | TYR | ASP | ASP | ALA | THR | LYS | ||||
| 6 | THR | PHE | THR | VAL | THR | GLU |
Samples:
sample_one: B3 domain of Streptococcal protein G, [U-15N], 1.8 mM
sample_conditions_one: pH: 5.8; temperature: 297 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N R1 | sample_one | not available | sample_conditions_one |
| 15N R2 | sample_one | not available | sample_conditions_one |
| 15N-1H NOE | sample_one | not available | sample_conditions_one |
Software:
XWINNMR v3.0 - NMR data processing
RELAXFIT - curve fitting, error estimation
AUTOPICK - peak picking, intensity calculation
NMR spectrometers:
- Bruker DRX 600 MHz