BMRB Entry 15283
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15283
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Citation: Nadaud, Philippe; Helmus, Jonathan; Jaroniec, Christopher. "13C and 15N chemical shift assignments and secondary structure of the B3 immunoglobulin-binding domain
of streptococcal protein G by magic-angle spinning solid-state NMR spectroscopy" Biomol. NMR Assignments 1, 117-120 (2007).
PubMed: 19636843
Assembly members:
GB3, polymer, 56 residues, 6207.8 Da.
Natural source: Common Name: Streptococcus sp. 'group G' Taxonomy ID: 1320 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptococcus Streptococcus sp. (group G)
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: n/a
Entity Sequences (FASTA):
GB3: MQYKLVINGKTLKGETTTKA
VDAETAEKAFKQYANDNGVD
GVWTYDDATKTFTVTE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 272 |
| 15N chemical shifts | 69 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GB3 | 1 |
Entities:
Entity 1, GB3 56 residues - 6207.8 Da.
| 1 | MET | GLN | TYR | LYS | LEU | VAL | ILE | ASN | GLY | LYS | ||||
| 2 | THR | LEU | LYS | GLY | GLU | THR | THR | THR | LYS | ALA | ||||
| 3 | VAL | ASP | ALA | GLU | THR | ALA | GLU | LYS | ALA | PHE | ||||
| 4 | LYS | GLN | TYR | ALA | ASN | ASP | ASN | GLY | VAL | ASP | ||||
| 5 | GLY | VAL | TRP | THR | TYR | ASP | ASP | ALA | THR | LYS | ||||
| 6 | THR | PHE | THR | VAL | THR | GLU |
Samples:
sample_1: GB3, [U-100% 13C; U-100% 15N], 20 mg; 2-methylpentane-2,4-diol 0.5 v/v; isopropanol 0.25 v/v; H2O 0.25 v/v; sodium phosphate 50 mM; sodium azide 0.02%
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 278 K; temperature controller setting: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D CC (DARR mixing) | sample_1 | solid | sample_conditions_1 |
| 2D N(CA)CX | sample_1 | solid | sample_conditions_1 |
| 2D N(CO)CX | sample_1 | solid | sample_conditions_1 |
| 3D NCACX | sample_1 | solid | sample_conditions_1 |
| 3D NCOCX | sample_1 | solid | sample_conditions_1 |
| 3D CONCA | sample_1 | solid | sample_conditions_1 |
Software:
VNMRJ v2.1B, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - data analysis
NMR spectrometers:
- Varian Varian NMR System 500 MHz