BMRB Entry 5792

Title:
Assignment of the 1H, 15N and 13C resonances of E. coli frataxin orthologue, CyaY
Deposition date:
2003-05-06
Original release date:
2004-06-24
Authors:
Nair, Margie; Adinolfi, Salvatore; Kelly, Geoff; Frenkiel, Thomas; Pastore, Annalisa
Citation:

Citation: Nair, Margie; Adinolfi, Salvatore; Kelly, Geoff; Frenkiel, Thomas; Pastore, Annalisa. "Letter to the Editor: NMR assignment of the 1H, 15N and 13C resonances of E. coli frataxin orthologue, CyaY "  J. Biomol. NMR 27, 403-404 (2003).
PubMed: 14512742

Assembly members:

Assembly members:
Escherichia coli CyaY, polymer, 109 residues, 12415 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts434
15N chemical shifts116
1H chemical shifts691

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CyaY monomer1

Entities:

Entity 1, CyaY monomer 109 residues - 12415 Da.

1   GLYALAMETGLYASNASPSERGLUPHEHIS
2   ARGLEUALAASPGLNLEUTRPLEUTHRILE
3   GLUGLUARGLEUASPASPTRPASPGLYASP
4   SERASPILEASPCYSGLUILEASNGLYGLY
5   VALLEUTHRILETHRPHEGLUASNGLYSER
6   LYSILEILEILEASNARGGLNGLUPROLEU
7   HISGLNVALTRPLEUALATHRLYSGLNGLY
8   GLYTYRHISPHEASPLEULYSGLYASPGLU
9   TRPILECYSASPARGSERGLYGLUTHRPHE
10   TRPASPLEULEUGLUGLNALAALATHRGLN
11   GLNALAGLYGLUTHRVALSERPHEARG

Samples:

15N_sample: Escherichia coli CyaY, [U-15N], 1 – 2 mM

13C_sample: Escherichia coli CyaY, [U-13C; U-15N], 1 – 2 mM

condition_set-1: pH*: 7; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-1H-15N NOESY-HSQCnot availablenot availablecondition_set-1
1H-1H-13C TOCSY-HSQCnot availablenot availablecondition_set-1
3D HNHAnot availablenot availablecondition_set-1
3D HNHBnot availablenot availablecondition_set-1
3D HNCAnot availablenot availablecondition_set-1
3D HN(CO)CAnot availablenot availablecondition_set-1
3D HNC0not availablenot availablecondition_set-1
3D CBCA(CO)NHnot availablenot availablecondition_set-1
1H-1H-13C HCCH-TOCSYnot availablenot availablecondition_set-1
1H-1H-13C NOESY-HSQCnot availablenot availablecondition_set-1
HCC(CO)NHnot availablenot availablecondition_set-1

Software:

XEASY v1.2 - peak assignment

NMRPipe - spectral processing

NMR spectrometers:

  • Varian UnityPlus 500 MHz
  • Varian Unity 600 MHz
  • Varian Inova 600 MHz
  • Varian Inova 800 MHz

Related Database Links:

BMRB 15237 15244
PDB
DBJ BAB38160 BAE77494 BAG79612 BAI27941 BAI33064
EMBL CAA47281 CAP78250 CAQ34148 CAQ91159 CAR00776
GB AAA67603 AAC76810 AAG58999 AAN45316 AAN83159
REF NP_290435 NP_312764 NP_418251 NP_709609 NP_756585
SP A1AHX3 A7ZU09 A8A6R1 B1IW97 B1LLX7
AlphaFold A1AHX3 A7ZU09 A8A6R1 B1IW97 B1LLX7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks