BMRB Entry 15244

Name: 1H, 15N chemical shift backbone assignment of the E19K,D22K mutant of the protein CyaY
Published: 2007-05-22

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15244

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N chemical shift backbone assignment of the E19K,D22K mutant of the protein CyaY

Deposition date:

2007-05-09

Original release date:

2007-05-22

Authors:

Pastore, Chiara; Pastore, Annalisa; Correia, Ana; Gomes, Claudio

Citation:

Citation: Adinolfi, Salvatore; Iannuzzi, Clara; Prischi, Filippo; Pastore, Chiara; Iametti, Stefania; Martin, Stephen; Bonomi, Franco; Pastore, Annalisa. "Bacterial frataxin CyaY is the gatekeeper of iron-sulfur cluster formation catalyzed by IscS." Nat. Struct. Mol. Biol. 16, 390-396 (2009).
PubMed: 19305405

Assembly members:

Assembly members:
CyaYE19K,D22K, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CyaYE19K,D22K: GAMNDSEFHRLADQLWLTIE KRLKDWDGDSDIDCEINGGV LTITFENGSKIIINRQEPLH QVWLATKQGGYHFDLKGDEW ICDRSGETFWDLLEQAATQQ AGETVSFR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	102
1H chemical shifts	102

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CyaYE19K,D22K	1

Entities:

Entity 1, CyaYE19K,D22K 108 residues - Formula weight is not available

1

GLY

ALA

MET

ASN

ASP

SER

GLU

PHE

HIS

ARG

2

LEU

ALA

ASP

GLN

LEU

TRP

LEU

THR

ILE

GLU

3

LYS

ARG

LEU

LYS

ASP

TRP

ASP

GLY

ASP

SER

4

ASP

ILE

ASP

CYS

GLU

ILE

ASN

GLY

VAL

5

LEU

THR

ILE

THR

PHE

GLU

ASN

GLY

SER

LYS

6

ILE

ASN

ARG

GLN

GLU

PRO

LEU

HIS

7

GLN

VAL

TRP

LEU

ALA

THR

LYS

GLN

GLY

8

TYR

HIS

PHE

ASP

LEU

LYS

GLY

ASP

GLU

TRP

9

ILE

CYS

ASP

ARG

SER

GLY

GLU

THR

PHE

TRP

10

ASP

LEU

GLU

GLN

ALA

THR

GLN

11

ALA

GLY

GLU

THR

VAL

SER

PHE

ARG

Samples:

sample_1: CyaYE19K,D22K, [U-98% 15N], 0.7 mM; Tris-HCl 20 mM; NaCl 50 mM

sample_conditions_1: pH: 7.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	15237
PDB	1EW4 1SOY 2EFF 2P1X
DBJ	BAB38160 BAE77494 BAG79612 BAI27941 BAI33064
EMBL	CAA47281 CAP78250 CAQ34148 CAQ91159 CAR00776
GB	AAA67603 AAC76810 AAG58999 AAN45316 AAP18882
REF	NP_312764 NP_418251 NP_709609 WP_000999934 WP_000999936
SP	A1AHX3 A7ZU09 A8A6R1 B1IW97 B1LLX7
AlphaFold	A1AHX3 A7ZU09 A8A6R1 B1IW97 B1LLX7