BMRB Entry 5715
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5715
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Citation: Jensen, Malene; Lauritzen, Connie; Dahl, Soren; Pedersen, John; Led, Jens. "Binding ability of a HHP-tagged protein towards Ni(2+) studied by
paramagnetic NMR relaxation: The possibility of obtaining long-range
structure information." J. Biomol. NMR 29, 175-185 (2004).
PubMed: 15014231
Assembly members:
HHP-tagged E. coli Thioredoxin, polymer, 111 residues, Formula weight is not available
NI, non-polymer, 58.693 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
HHP-tagged E. coli Thioredoxin: HHPSDKIIHLTDDSFDTDVL
KADGAILVDFWAEWCGPCKM
IAPILDEIADEYQGKLTVAK
LNIDQNPGTAPKYGIRGIPT
LLLFKNGEVAATKVGALSKG
QLKEFLDANLA
- heteronucl_T1_relaxation
| Data type | Count |
| T1 relaxation values | 84 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HHP-Trx unit 1 | 1 |
| 2 | HHP-Trx unit 2 | 1 |
| 3 | HHP-Trx unit 3 | 1 |
| 4 | Nickel ion | 2 |
Entities:
Entity 1, HHP-Trx unit 1 111 residues - Formula weight is not available
| 1 | HIS | HIS | PRO | SER | ASP | LYS | ILE | ILE | HIS | LEU | ||||
| 2 | THR | ASP | ASP | SER | PHE | ASP | THR | ASP | VAL | LEU | ||||
| 3 | LYS | ALA | ASP | GLY | ALA | ILE | LEU | VAL | ASP | PHE | ||||
| 4 | TRP | ALA | GLU | TRP | CYS | GLY | PRO | CYS | LYS | MET | ||||
| 5 | ILE | ALA | PRO | ILE | LEU | ASP | GLU | ILE | ALA | ASP | ||||
| 6 | GLU | TYR | GLN | GLY | LYS | LEU | THR | VAL | ALA | LYS | ||||
| 7 | LEU | ASN | ILE | ASP | GLN | ASN | PRO | GLY | THR | ALA | ||||
| 8 | PRO | LYS | TYR | GLY | ILE | ARG | GLY | ILE | PRO | THR | ||||
| 9 | LEU | LEU | LEU | PHE | LYS | ASN | GLY | GLU | VAL | ALA | ||||
| 10 | ALA | THR | LYS | VAL | GLY | ALA | LEU | SER | LYS | GLY | ||||
| 11 | GLN | LEU | LYS | GLU | PHE | LEU | ASP | ALA | ASN | LEU | ||||
| 12 | ALA |
Entity 2, Nickel ion - Ni - 58.693 Da.
| 1 | NI |
Samples:
sample_1: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM
sample_2: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 0.1 mM
sample_3: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 0.2 mM
sample_4: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 0.3 mM
sample_5: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 0.4 mM
sample_6: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 0.5 mM
sample_7: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 0.6 mM
sample_8: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 0.7 mM
sample_9: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 0.8 mM
sample_10: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 0.9 mM
sample_11: HHP-tagged E. coli Thioredoxin, [U-15N], 1.0 mM; Nickel ion 1.0 mM
Condition_1: pH: 7.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| Inversion recovery 1H-15N HSQC experiments | not available | not available | Condition_1 |
Software:
No software information available
NMR spectrometers:
- Varian Inova 500 MHz
Related Database Links:
| BMRB | 17700 1812 1813 62 |
| PDB | |
| DBJ | BAA00903 BAB38137 BAE77517 BAG79587 BAH61053 |
| EMBL | CAA79851 CAD09400 CAP78228 CAQ34125 CAQ91183 |
| GB | AAA24533 AAA24534 AAA24693 AAA24694 AAA24696 |
| PIR | AF0922 B91218 C86064 |
| REF | NP_290411 NP_312741 NP_418228 NP_457831 NP_462806 |
| SP | P0AA25 P0AA26 P0AA27 P0AA28 P0AA29 |
| AlphaFold | P0AA25 P0AA26 P0AA27 P0AA28 P0AA29 |