BMRB Entry 5653
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5653
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Katayama, Hidekazu. "The solution structure of molt-inhibiting hormone from the kuruma prawn
Marsupenaeus japonicus" J. Biol. Chem. ., .-..
Assembly members:
molt-inhibiting hormone, polymer, 78 residues, 9134 Da.
Natural source: Common Name: kuruma prawn Taxonomy ID: 27405 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Marsupenaeus japonicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a(+)
Entity Sequences (FASTA):
molt-inhibiting hormone: ASFIDNTCRGVMGNRDIYKK
VVRVCEDCTNIFRLPGLDGM
CRNRCFYNEWFLICLKAANR
EDEIEKFRVWISILNAGQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 528 |
| 13C chemical shifts | 343 |
| 15N chemical shifts | 93 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MIH monomer | 1 |
Entities:
Entity 1, MIH monomer 78 residues - 9134 Da.
| 1 | ALA | SER | PHE | ILE | ASP | ASN | THR | CYS | ARG | GLY | ||||
| 2 | VAL | MET | GLY | ASN | ARG | ASP | ILE | TYR | LYS | LYS | ||||
| 3 | VAL | VAL | ARG | VAL | CYS | GLU | ASP | CYS | THR | ASN | ||||
| 4 | ILE | PHE | ARG | LEU | PRO | GLY | LEU | ASP | GLY | MET | ||||
| 5 | CYS | ARG | ASN | ARG | CYS | PHE | TYR | ASN | GLU | TRP | ||||
| 6 | PHE | LEU | ILE | CYS | LEU | LYS | ALA | ALA | ASN | ARG | ||||
| 7 | GLU | ASP | GLU | ILE | GLU | LYS | PHE | ARG | VAL | TRP | ||||
| 8 | ILE | SER | ILE | LEU | ASN | ALA | GLY | GLN |
Samples:
sample_1: molt-inhibiting hormone, [U-15N; U-13C], 1.5 – 2.0 mM
sample_2: molt-inhibiting hormone, [U-15N], 1.5 – 2.0 mM
Ex-cond_1: pH: 7.4; temperature: 298 K; ionic strength: 0 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCO | not available | not available | not available |
| (HCA)CO(CA)NH | not available | not available | not available |
| C(CO)NH | not available | not available | not available |
| H(CCO)NH | not available | not available | not available |
| HCCH-COSY | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| 13C-edited NOESY | not available | not available | not available |
| 15N-edited NOESY | not available | not available | not available |
Software:
nmrPipe v1.7 -
SPARKY v3.76 - peak picking
NMR spectrometers:
- Varian INOVA 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks