BMRB Entry 5505

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5505

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Dynamics of Reduced Plastocyanin from the Cyanobacterium Anabaena variabilis: Regions Involved in Electron Transfer have Enhanched Mobility

Deposition date:

2002-08-22

Original release date:

2011-08-10

Authors:

Ma, Lixin; Hass, Mathias; Rasmussen, Nanna; Kristensen, Soren; Ulstrup, Jens; Led, Jens

Citation:

Citation: Ma, Lixin; Hass, Mathias; Vierick, Nanna; Kristensen, Soren; Ulstrup, Jens; Led, Jens. "Backbone Dynamics of Reduced Plastocyanin from the Cyanobacterium Anabaena variabilis: Regions Involved in Electron Transfer have Enhanched Mobility" Biochemistry 42, 320-330 (2003).
PubMed: 12525159

Assembly members:

Assembly members:
Cu(II) Plastocyanin from Anabeana Variabilis, polymer, 105 residues, 10500 Da.
COPPER (I) ION, non-polymer, 63.546 Da.

Natural source:

Natural source: Common Name: Anabaena variabilis Taxonomy ID: 1172 Superkingdom: Eubacteria Kingdom: not available Genus/species: Anabaena variabilis

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cu(II) Plastocyanin from Anabeana Variabilis: ETYTVKLGSDKGLLVFEPAK LTIKPGDTVEFLNNKVPPHN VVFDAALNPAKSADLAKSLS HKQLLMSPGQSTSTTFPADA PAGEYTFYCEPHRGAGMVGK ITVAG

Data sets:

assigned_chemical_shifts
- chemshift_1
- chemshift_2
heteronucl_T1_relaxation
- T1_relaxation
heteronucl_T2_relaxation
- T2_relaxation_1
heteronucl_NOEs
- heteronuclear_NOE

Data type	Count
1H chemical shifts	673
13C chemical shifts	429
15N chemical shifts	104
T1 relaxation values	163
T2 relaxation values	163

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Plastocyanin	1
2	COPPER (I) ION	2

Entities:

Entity 1, Plastocyanin 105 residues - 10500 Da.

1

GLU

THR

TYR

THR

VAL

LYS

LEU

GLY

SER

ASP

2

LYS

GLY

LEU

VAL

PHE

GLU

PRO

ALA

LYS

3

LEU

THR

ILE

LYS

PRO

GLY

ASP

THR

VAL

GLU

4

PHE

LEU

ASN

LYS

VAL

PRO

HIS

ASN

5

VAL

PHE

ASP

ALA

LEU

ASN

PRO

ALA

6

LYS

SER

ALA

ASP

LEU

ALA

LYS

SER

LEU

SER

7

HIS

LYS

GLN

LEU

MET

SER

PRO

GLY

GLN

8

SER

THR

SER

THR

PHE

PRO

ALA

ASP

ALA

9

PRO

ALA

GLY

GLU

TYR

THR

PHE

TYR

CYS

GLU

10

PRO

HIS

ARG

GLY

ALA

GLY

MET

VAL

GLY

LYS

11

ILE

THR

VAL

ALA

GLY

Entity 2, COPPER (I) ION - Cu - 63.546 Da.

1

CU1

Samples:

sample_1: Cu(II) Plastocyanin from Anabeana Variabilis, [U-95% 15N], 4.1 mM; COPPER (I) ION 4.1 mM; NaCl 100 mM; H2O 90%; D2O 10%; sodium ascorbate0.6 – 0.7 mM

sample_2: Cu(II) Plastocyanin from Anabeana Variabilis, [U-95% 15N], 2.8 – 3.2 mM; COPPER (I) ION2.8 – 3.2 mM; H2O 90%; D2O 10%

cond_set_1: pH: 7.0 na; temperature: 298 K; ionic strength: 100 mM

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	not available	not available	not available
HSQC-TOCSY	not available	not available	not available
HNCA	not available	not available	not available
1H-13C HSQC	not available	not available	not available
HSQC	not available	not available	not available
HMQC-COSY	not available	not available	not available
HNCO	not available	not available	not available
3D TOCSY-HMQC	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Varian UnityInova 500 MHz
Varian UnityInova 750 MHz
Bruker AMX 500 MHz

BMRB	4738 5858
PDB	1FA4 1NIN 1TU2 2CJ3 2GIM
DBJ	BAB77782
EMBL	CAA05338 CAA32527
GB	AAA59364 ABA22358
PIR	CUAI S06999
REF	NP_484302 WP_010994435 WP_011319499 YP_323253
SP	O52830 P0C178 P46444 Q3M9H8
AlphaFold	O52830 P0C178 P46444 Q3M9H8