BMRB Entry 4738
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4738
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NMR-STAR v3 text file.
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Citation: Ma, Lixin; Led, Jens. "Determination by High Field NMR Spectroscopy of the Longitudinal Electron
Relaxation Rate in Cu(II)Plastocyanin from Anabaena variablis." J. Am. Chem. Soc. 122, 7823-7824 (2000).
Assembly members:
Plastocyanin from Anabena variabilis, polymer, 105 residues, 10500 Da.
COPPER (II) ION, non-polymer, 63.546 Da.
COPPER (I) ION, non-polymer, 63.546 Da.
Natural source: Common Name: Anabaena variabilis Taxonomy ID: 1172 Superkingdom: Bacteria Kingdom: not available Genus/species: Anabaena variabilis
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
Plastocyanin from Anabena variabilis: ETYTVKLGSDKGLLVFEPAK
LTIKPGDTVEFLNNKVPPHN
VVFDAALNPAKSADLAKSLS
HKQLLMSPGQSTSTTFPADA
PAGEYTFYCEPHRGAGMVGK
ITVAG
- other_data_list
| Data type | Count |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | plastocyanin peptide | 1 |
| 2 | Cu(II) | 2 |
| 3 | Cu(I) | 3 |
Entities:
Entity 1, plastocyanin peptide 105 residues - 10500 Da.
| 1 | GLU | THR | TYR | THR | VAL | LYS | LEU | GLY | SER | ASP | ||||
| 2 | LYS | GLY | LEU | LEU | VAL | PHE | GLU | PRO | ALA | LYS | ||||
| 3 | LEU | THR | ILE | LYS | PRO | GLY | ASP | THR | VAL | GLU | ||||
| 4 | PHE | LEU | ASN | ASN | LYS | VAL | PRO | PRO | HIS | ASN | ||||
| 5 | VAL | VAL | PHE | ASP | ALA | ALA | LEU | ASN | PRO | ALA | ||||
| 6 | LYS | SER | ALA | ASP | LEU | ALA | LYS | SER | LEU | SER | ||||
| 7 | HIS | LYS | GLN | LEU | LEU | MET | SER | PRO | GLY | GLN | ||||
| 8 | SER | THR | SER | THR | THR | PHE | PRO | ALA | ASP | ALA | ||||
| 9 | PRO | ALA | GLY | GLU | TYR | THR | PHE | TYR | CYS | GLU | ||||
| 10 | PRO | HIS | ARG | GLY | ALA | GLY | MET | VAL | GLY | LYS | ||||
| 11 | ILE | THR | VAL | ALA | GLY |
Entity 2, Cu(II) - Cu - 63.546 Da.
| 1 | CU |
Entity 3, Cu(I) - Cu - 63.546 Da.
| 1 | CU1 |
Samples:
sample_one: Plastocyanin from Anabena variabilis 2.9 mM; COPPER (I) ION 2.9 mM
sample_two: Plastocyanin from Anabena variabilis 2.9 mM; COPPER (I) ION 2.38 mM; COPPER (II) ION 0.52 mM
sample_three: Plastocyanin from Anabena variabilis 2.9 mM; COPPER (I) ION 2.45 mM; COPPER (II) ION 0.45 mM
cond_set_1: pH: 7.50; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| unknown | not available | isotropic | cond_set_1 |
Software:
No software information available
NMR spectrometers:
- Varian Inova 500 MHz
- Varian Inova 750 MHz