BMRB Entry 54023

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR54023

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Title:
Chemical shifts of Tubulin beta (Tub2p) C-terminal tail from S. cerevisiae
Deposition date:
2026-09-18
Original release date:
2026-10-20
Authors:
Whited, Allison; DeLear, Patrick; Thomas, Ezekiel; Allen, Jeffre; Ferrer-Imbert, Genesis; Acharya, Nirbhik; Castaneda, Carlos; Sept, David; Moore, Jeffrey; Hough, Loren
Citation:

Citation: Whited, Allison; DeLear, Patrick; Thomas, Ezekiel; Allen, Jeffre; Ferrer-Imbert, Genesis; Acharya, Nirbhik; Castaneda, Carlos; Sept, David; Moore, Jeffrey; Hough, Loren. "Tubulin C-terminal tails are pH sensors that regulate microtubule function "  J. Biol. Chem. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 34 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEXT 4T-3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSQEATVEDDEEVDENGDFG APQNQDEPITENFE

Data sets:
Data typeCount
13C chemical shifts88
15N chemical shifts30
1H chemical shifts30

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Tub2p C-terminal tail1

Entities:

Entity 1, Tub2p C-terminal tail 34 residues - Formula weight is not available

Author sequence numbering retains the wild-type scheme

1   GLYSERGLNGLUALATHRVALGLUASPASP
2   GLUGLUVALASPGLUASNGLYASPPHEGLY
3   ALAPROGLNASNGLNASPGLUPROILETHR
4   GLUASNPHEGLU

Samples:

sample_1: Tub2p C-terminal tail, [U-99% 13C; U-99% 15N], 275 uM; PIPES 20 mM; EGTA 1 mM; MgCl2 1 mM; TSP 15 uM

sample_conditions_1: ionic strength: 20 mM; pH: 7; pressure: 1 atm; temperature: 283.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CCCONHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

CcpNMR - chemical shift assignment, data analysis

NMR spectrometers:

  • Agilent INOVA 600 MHz

Related Database Links:

UNP P02557
AlphaFold P02557

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks