data_54023 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 54023 _Entry.Title ; Chemical shifts of Tubulin beta (Tub2p) C-terminal tail from S. cerevisiae ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2026-09-18 _Entry.Accession_date 2026-09-18 _Entry.Last_release_date 2026-09-18 _Entry.Original_release_date 2026-09-18 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID . _Entry.Generated_software_label . _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Allison Whited . M. . . 54023 2 Patrick DeLear . . . . 54023 3 Ezekiel Thomas . C. . . 54023 4 Jeffre Allen . . . . 54023 5 Genesis Ferrer-Imbert . . . . 54023 6 Nirbhik Acharya . . . 0000-0003-4591-5215 54023 7 Carlos Castaneda . A. . 0000-0001-9634-0867 54023 8 David Sept . . . . 54023 9 Jeffrey Moore . K. . . 54023 10 Loren Hough . . . 0000-0002-1104-0126 54023 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 54023 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '13C chemical shifts' 88 54023 '15N chemical shifts' 30 54023 '1H chemical shifts' 30 54023 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2026-10-20 . original BMRB . 54023 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 54016 'Chemical shifts of Tubulin alpha C-terminal tail from T. thermophila' 54023 BMRB 54017 'Chemical shifts of TUBB C-terminal tail from H. sapiens' 54023 BMRB 54018 'Chemical shifts of Tubulin beta BTU1 C-terminal tail from T. thermophila' 54023 BMRB 54019 'Chemical shifts of Tubulin beta-1 (MEC-7) C-terminal tail from C. elegans' 54023 BMRB 54020 'Chemical shifts of Tubulin beta-4 (TBB-4) C-terminal tail from C. elegans' 54023 BMRB 54021 'Chemical shifts of TUBB3 C-terminal tail from H. sapiens' 54023 BMRB 54022 'Chemical shifts of Tubulin alpha-1 (Tub1p) C-terminal tail from S. cerevisiae' 54023 BMRB 54024 'Chemical shifts of TUBA1A C-terminal tail from H. sapiens' 54023 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 54023 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID . _Citation.DOI . _Citation.Full_citation . _Citation.Title ; Tubulin C-terminal tails are pH sensors that regulate microtubule function ; _Citation.Status submitted _Citation.Type journal _Citation.Journal_abbrev 'J. Biol. Chem.' _Citation.Journal_name_full . _Citation.Journal_volume . _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first . _Citation.Page_last . _Citation.Year . _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Allison Whited . M. . . 54023 1 2 Patrick DeLear . . . . 54023 1 3 Ezekiel Thomas . C. . . 54023 1 4 Jeffre Allen . . . . 54023 1 5 Genesis Ferrer-Imbert . . . . 54023 1 6 Nirbhik Acharya . . . . 54023 1 7 Carlos Castaneda . A. . . 54023 1 8 David Sept . . . . 54023 1 9 Jeffrey Moore . K. . . 54023 1 10 Loren Hough . . . . 54023 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 54023 _Assembly.ID 1 _Assembly.Name 'Tub2p C-terminal tail' _Assembly.BMRB_code . _Assembly.Number_of_components 1 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states no _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass 3756 _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 'Tub2p C-terminal tail' 1 $entity_1 . . yes native no no . . . 54023 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 54023 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; GSQEATVEDDEEVDENGDFG APQNQDEPITENFE ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq ; 426, Q 427, E 428, A 429, T 430, V 431, E 432, D 433, D 434, E 435, E 436, V 437, D 438, E 439, N 440, G 441, D 442, F 443, G 444, A 445, P 446, Q 447, N 448, Q 449, D 450, E 451, P 452, I 453, T 454, E 455, N 456, F 457, E ; _Entity.Polymer_author_seq_details 'Author sequence numbering retains the wild-type scheme' _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 34 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment 'C-terminal tail' _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 yes UNP P02557 . Tub2p . . . . . . . . . . . . . . 54023 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 424 GLY . 54023 1 2 425 SER . 54023 1 3 426 GLN . 54023 1 4 427 GLU . 54023 1 5 428 ALA . 54023 1 6 429 THR . 54023 1 7 430 VAL . 54023 1 8 431 GLU . 54023 1 9 432 ASP . 54023 1 10 433 ASP . 54023 1 11 434 GLU . 54023 1 12 435 GLU . 54023 1 13 436 VAL . 54023 1 14 437 ASP . 54023 1 15 438 GLU . 54023 1 16 439 ASN . 54023 1 17 440 GLY . 54023 1 18 441 ASP . 54023 1 19 442 PHE . 54023 1 20 443 GLY . 54023 1 21 444 ALA . 54023 1 22 445 PRO . 54023 1 23 446 GLN . 54023 1 24 447 ASN . 54023 1 25 448 GLN . 54023 1 26 449 ASP . 54023 1 27 450 GLU . 54023 1 28 451 PRO . 54023 1 29 452 ILE . 54023 1 30 453 THR . 54023 1 31 454 GLU . 54023 1 32 455 ASN . 54023 1 33 456 PHE . 54023 1 34 457 GLU . 54023 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . GLY 1 1 54023 1 . SER 2 2 54023 1 . GLN 3 3 54023 1 . GLU 4 4 54023 1 . ALA 5 5 54023 1 . THR 6 6 54023 1 . VAL 7 7 54023 1 . GLU 8 8 54023 1 . ASP 9 9 54023 1 . ASP 10 10 54023 1 . GLU 11 11 54023 1 . GLU 12 12 54023 1 . VAL 13 13 54023 1 . ASP 14 14 54023 1 . GLU 15 15 54023 1 . ASN 16 16 54023 1 . GLY 17 17 54023 1 . ASP 18 18 54023 1 . PHE 19 19 54023 1 . GLY 20 20 54023 1 . ALA 21 21 54023 1 . PRO 22 22 54023 1 . GLN 23 23 54023 1 . ASN 24 24 54023 1 . GLN 25 25 54023 1 . ASP 26 26 54023 1 . GLU 27 27 54023 1 . PRO 28 28 54023 1 . ILE 29 29 54023 1 . THR 30 30 54023 1 . GLU 31 31 54023 1 . ASN 32 32 54023 1 . PHE 33 33 54023 1 . GLU 34 34 54023 1 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 54023 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 4932 organism . 'Saccharomyces cerevisiae' "baker's yeast" . . Eukaryota Fungi Saccharomyces cerevisiae . . . . . . . . . . . . . 54023 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 54023 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . 'pGEXT 4T-3' . . . 54023 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 54023 _Sample.ID 1 _Sample.Name 13C_15N_Tub2p_tail _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'Tub2p C-terminal tail' '[U-99% 13C; U-99% 15N]' . . 1 $entity_1 . . 275 50 500 uM . . . . 54023 1 2 PIPES 'natural abundance' . . . . . . 20 . . mM . . . . 54023 1 3 EGTA 'natural abundance' . . . . . . 1 . . mM . . . . 54023 1 4 MgCl2 'natural abundance' . . . . . . 1 . . mM . . . . 54023 1 5 TSP 'natural abundance' . . . . . . 15 . . uM . . . . 54023 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 54023 _Sample_condition_list.ID 1 _Sample_condition_list.Name 'Buffer conditions' _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 20 . mM 54023 1 pH 7 . pH 54023 1 pressure 1 . atm 54023 1 temperature 283.15 . K 54023 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 54023 _Software.ID 1 _Software.Type . _Software.Name NMRPipe _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID processing . 54023 1 stop_ save_ save_software_2 _Software.Sf_category software _Software.Sf_framecode software_2 _Software.Entry_ID 54023 _Software.ID 2 _Software.Type . _Software.Name CcpNMR _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'chemical shift assignment' . 54023 2 'data analysis' . 54023 2 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 54023 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name 'Agilent 600MHz INOVA spectrometer' _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Agilent _NMR_spectrometer.Model INOVA _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 54023 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-15N HSQC' no no no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 54023 1 2 '3D CCCONH' no yes no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 54023 1 3 '3D HNCACB' no yes no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 54023 1 4 '3D HNCO' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 54023 1 5 '3D HNCACO' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 54023 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chem_shift_reference_1 _Chem_shift_reference.Entry_ID 54023 _Chem_shift_reference.ID 1 _Chem_shift_reference.Name Reference _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID C 13 TSP 'methyl protons' . . . . ppm 0.00 na indirect 0.251449530 . . . . . 54023 1 H 1 TSP 'methyl protons' . . . . ppm 0.00 internal direct 1.000000000 . . . . . 54023 1 N 15 TSP 'methyl protons' . . . . ppm 0.00 na indirect 0.101329118 . . . . . 54023 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_1 _Assigned_chem_shift_list.Entry_ID 54023 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Name 'Tub2p C-terminal tail' _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 2 '3D CCCONH' . . . 54023 1 3 '3D HNCACB' . . . 54023 1 4 '3D HNCO' . . . 54023 1 5 '3D HNCACO' . . . 54023 1 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 1 $software_1 . . 54023 1 2 $software_2 . . 54023 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 . 1 3 3 GLN H H 1 8.705 . . . . . . . . 426 Q HN . 54023 1 2 . 1 . 1 3 3 GLN C C 13 175.958 . . . . . . . . 426 Q C . 54023 1 3 . 1 . 1 3 3 GLN CA C 13 56.272 . . . . . . . . 426 Q CA . 54023 1 4 . 1 . 1 3 3 GLN CB C 13 29.160 . . . . . . . . 426 Q CB . 54023 1 5 . 1 . 1 3 3 GLN N N 15 122.274 . . . . . . . . 426 Q N . 54023 1 6 . 1 . 1 4 4 GLU H H 1 8.470 . . . . . . . . 427 E HN . 54023 1 7 . 1 . 1 4 4 GLU C C 13 176.044 . . . . . . . . 427 E C . 54023 1 8 . 1 . 1 4 4 GLU CA C 13 56.635 . . . . . . . . 427 E CA . 54023 1 9 . 1 . 1 4 4 GLU CB C 13 30.081 . . . . . . . . 427 E CB . 54023 1 10 . 1 . 1 4 4 GLU N N 15 121.803 . . . . . . . . 427 E N . 54023 1 11 . 1 . 1 5 5 ALA H H 1 8.413 . . . . . . . . 428 A HN . 54023 1 12 . 1 . 1 5 5 ALA C C 13 177.760 . . . . . . . . 428 A C . 54023 1 13 . 1 . 1 5 5 ALA CA C 13 52.604 . . . . . . . . 428 A CA . 54023 1 14 . 1 . 1 5 5 ALA CB C 13 18.932 . . . . . . . . 428 A CB . 54023 1 15 . 1 . 1 5 5 ALA N N 15 125.083 . . . . . . . . 428 A N . 54023 1 16 . 1 . 1 6 6 THR H H 1 8.300 . . . . . . . . 429 T HN . 54023 1 17 . 1 . 1 6 6 THR C C 13 174.517 . . . . . . . . 429 T C . 54023 1 18 . 1 . 1 6 6 THR CA C 13 62.007 . . . . . . . . 429 T CA . 54023 1 19 . 1 . 1 6 6 THR CB C 13 70.337 . . . . . . . . 429 T CB . 54023 1 20 . 1 . 1 6 6 THR N N 15 114.680 . . . . . . . . 429 T N . 54023 1 21 . 1 . 1 7 7 VAL H H 1 8.320 . . . . . . . . 430 V HN . 54023 1 22 . 1 . 1 7 7 VAL C C 13 176.087 . . . . . . . . 430 V C . 54023 1 23 . 1 . 1 7 7 VAL CA C 13 62.276 . . . . . . . . 430 V CA . 54023 1 24 . 1 . 1 7 7 VAL CB C 13 32.734 . . . . . . . . 430 V CB . 54023 1 25 . 1 . 1 7 7 VAL N N 15 122.974 . . . . . . . . 430 V N . 54023 1 26 . 1 . 1 8 8 GLU H H 1 8.612 . . . . . . . . 431 E HN . 54023 1 27 . 1 . 1 8 8 GLU C C 13 176.148 . . . . . . . . 431 E C . 54023 1 28 . 1 . 1 8 8 GLU CA C 13 56.439 . . . . . . . . 431 E CA . 54023 1 29 . 1 . 1 8 8 GLU CB C 13 30.174 . . . . . . . . 431 E CB . 54023 1 30 . 1 . 1 8 8 GLU N N 15 125.053 . . . . . . . . 431 E N . 54023 1 31 . 1 . 1 9 9 ASP H H 1 8.445 . . . . . . . . 432 D HN . 54023 1 32 . 1 . 1 9 9 ASP C C 13 175.993 . . . . . . . . 432 D C . 54023 1 33 . 1 . 1 9 9 ASP CA C 13 54.525 . . . . . . . . 432 D CA . 54023 1 34 . 1 . 1 9 9 ASP CB C 13 41.169 . . . . . . . . 432 D CB . 54023 1 35 . 1 . 1 9 9 ASP N N 15 121.869 . . . . . . . . 432 D N . 54023 1 36 . 1 . 1 10 10 ASP H H 1 8.397 . . . . . . . . 433 D HN . 54023 1 37 . 1 . 1 10 10 ASP C C 13 176.275 . . . . . . . . 433 D C . 54023 1 38 . 1 . 1 10 10 ASP CA C 13 54.371 . . . . . . . . 433 D CA . 54023 1 39 . 1 . 1 10 10 ASP CB C 13 41.141 . . . . . . . . 433 D CB . 54023 1 40 . 1 . 1 10 10 ASP N N 15 121.255 . . . . . . . . 433 D N . 54023 1 41 . 1 . 1 11 11 GLU H H 1 8.379 . . . . . . . . 434 E HN . 54023 1 42 . 1 . 1 11 11 GLU C C 13 176.438 . . . . . . . . 434 E C . 54023 1 43 . 1 . 1 11 11 GLU CA C 13 56.634 . . . . . . . . 434 E CA . 54023 1 44 . 1 . 1 11 11 GLU CB C 13 30.235 . . . . . . . . 434 E CB . 54023 1 45 . 1 . 1 11 11 GLU N N 15 121.275 . . . . . . . . 434 E N . 54023 1 46 . 1 . 1 12 12 GLU H H 1 8.513 . . . . . . . . 435 E HN . 54023 1 47 . 1 . 1 12 12 GLU C C 13 176.375 . . . . . . . . 435 E C . 54023 1 48 . 1 . 1 12 12 GLU CA C 13 56.533 . . . . . . . . 435 E CA . 54023 1 49 . 1 . 1 12 12 GLU CB C 13 30.055 . . . . . . . . 435 E CB . 54023 1 50 . 1 . 1 12 12 GLU N N 15 122.853 . . . . . . . . 435 E N . 54023 1 51 . 1 . 1 13 13 VAL H H 1 8.322 . . . . . . . . 436 V HN . 54023 1 52 . 1 . 1 13 13 VAL C C 13 175.768 . . . . . . . . 436 V C . 54023 1 53 . 1 . 1 13 13 VAL CA C 13 62.214 . . . . . . . . 436 V CA . 54023 1 54 . 1 . 1 13 13 VAL CB C 13 32.960 . . . . . . . . 436 V CB . 54023 1 55 . 1 . 1 13 13 VAL N N 15 122.210 . . . . . . . . 436 V N . 54023 1 56 . 1 . 1 14 14 ASP H H 1 8.568 . . . . . . . . 437 D HN . 54023 1 57 . 1 . 1 14 14 ASP C C 13 176.605 . . . . . . . . 437 D C . 54023 1 58 . 1 . 1 14 14 ASP CA C 13 54.065 . . . . . . . . 437 D CA . 54023 1 59 . 1 . 1 14 14 ASP CB C 13 41.258 . . . . . . . . 437 D CB . 54023 1 60 . 1 . 1 14 14 ASP N N 15 125.089 . . . . . . . . 437 D N . 54023 1 61 . 1 . 1 15 15 GLU H H 1 8.699 . . . . . . . . 438 E HN . 54023 1 62 . 1 . 1 15 15 GLU C C 13 176.553 . . . . . . . . 438 E C . 54023 1 63 . 1 . 1 15 15 GLU CA C 13 57.117 . . . . . . . . 438 E CA . 54023 1 64 . 1 . 1 15 15 GLU CB C 13 29.734 . . . . . . . . 438 E CB . 54023 1 65 . 1 . 1 15 15 GLU N N 15 123.038 . . . . . . . . 438 E N . 54023 1 66 . 1 . 1 16 16 ASN H H 1 8.597 . . . . . . . . 439 N HN . 54023 1 67 . 1 . 1 16 16 ASN C C 13 175.845 . . . . . . . . 439 N C . 54023 1 68 . 1 . 1 16 16 ASN CA C 13 53.580 . . . . . . . . 439 N CA . 54023 1 69 . 1 . 1 16 16 ASN CB C 13 39.112 . . . . . . . . 439 N CB . 54023 1 70 . 1 . 1 16 16 ASN N N 15 119.106 . . . . . . . . 439 N N . 54023 1 71 . 1 . 1 17 17 GLY H H 1 8.284 . . . . . . . . 440 G HN . 54023 1 72 . 1 . 1 17 17 GLY C C 13 173.674 . . . . . . . . 440 G C . 54023 1 73 . 1 . 1 17 17 GLY CA C 13 45.501 . . . . . . . . 440 G CA . 54023 1 74 . 1 . 1 17 17 GLY N N 15 109.279 . . . . . . . . 440 G N . 54023 1 75 . 1 . 1 18 18 ASP H H 1 8.258 . . . . . . . . 441 D HN . 54023 1 76 . 1 . 1 18 18 ASP C C 13 176.233 . . . . . . . . 441 D C . 54023 1 77 . 1 . 1 18 18 ASP CA C 13 54.295 . . . . . . . . 441 D CA . 54023 1 78 . 1 . 1 18 18 ASP CB C 13 41.075 . . . . . . . . 441 D CB . 54023 1 79 . 1 . 1 18 18 ASP N N 15 120.413 . . . . . . . . 441 D N . 54023 1 80 . 1 . 1 19 19 PHE H H 1 8.339 . . . . . . . . 442 F HN . 54023 1 81 . 1 . 1 19 19 PHE C C 13 176.494 . . . . . . . . 442 F C . 54023 1 82 . 1 . 1 19 19 PHE CA C 13 58.146 . . . . . . . . 442 F CA . 54023 1 83 . 1 . 1 19 19 PHE CB C 13 39.185 . . . . . . . . 442 F CB . 54023 1 84 . 1 . 1 19 19 PHE N N 15 121.025 . . . . . . . . 442 F N . 54023 1 85 . 1 . 1 20 20 GLY H H 1 8.375 . . . . . . . . 443 G HN . 54023 1 86 . 1 . 1 20 20 GLY C C 13 173.372 . . . . . . . . 443 G C . 54023 1 87 . 1 . 1 20 20 GLY CA C 13 45.030 . . . . . . . . 443 G CA . 54023 1 88 . 1 . 1 20 20 GLY N N 15 110.242 . . . . . . . . 443 G N . 54023 1 89 . 1 . 1 21 21 ALA H H 1 8.153 . . . . . . . . 444 A HN . 54023 1 90 . 1 . 1 21 21 ALA C C 13 175.634 . . . . . . . . 444 A C . 54023 1 91 . 1 . 1 21 21 ALA CA C 13 50.638 . . . . . . . . 444 A CA . 54023 1 92 . 1 . 1 21 21 ALA CB C 13 17.793 . . . . . . . . 444 A CB . 54023 1 93 . 1 . 1 21 21 ALA N N 15 124.979 . . . . . . . . 444 A N . 54023 1 94 . 1 . 1 23 23 GLN H H 1 8.676 . . . . . . . . 446 Q HN . 54023 1 95 . 1 . 1 23 23 GLN C C 13 175.861 . . . . . . . . 446 Q C . 54023 1 96 . 1 . 1 23 23 GLN CA C 13 55.714 . . . . . . . . 446 Q CA . 54023 1 97 . 1 . 1 23 23 GLN CB C 13 29.429 . . . . . . . . 446 Q CB . 54023 1 98 . 1 . 1 23 23 GLN N N 15 120.969 . . . . . . . . 446 Q N . 54023 1 99 . 1 . 1 24 24 ASN H H 1 8.626 . . . . . . . . 447 N HN . 54023 1 100 . 1 . 1 24 24 ASN C C 13 175.212 . . . . . . . . 447 N C . 54023 1 101 . 1 . 1 24 24 ASN CA C 13 53.427 . . . . . . . . 447 N CA . 54023 1 102 . 1 . 1 24 24 ASN CB C 13 38.911 . . . . . . . . 447 N CB . 54023 1 103 . 1 . 1 24 24 ASN N N 15 120.300 . . . . . . . . 447 N N . 54023 1 104 . 1 . 1 25 25 GLN H H 1 8.593 . . . . . . . . 448 Q HN . 54023 1 105 . 1 . 1 25 25 GLN C C 13 175.563 . . . . . . . . 448 Q C . 54023 1 106 . 1 . 1 25 25 GLN CA C 13 55.868 . . . . . . . . 448 Q CA . 54023 1 107 . 1 . 1 25 25 GLN CB C 13 29.353 . . . . . . . . 448 Q CB . 54023 1 108 . 1 . 1 25 25 GLN N N 15 121.182 . . . . . . . . 448 Q N . 54023 1 109 . 1 . 1 26 26 ASP H H 1 8.472 . . . . . . . . 449 D HN . 54023 1 110 . 1 . 1 26 26 ASP C C 13 175.949 . . . . . . . . 449 D C . 54023 1 111 . 1 . 1 26 26 ASP CA C 13 56.635 . . . . . . . . 449 D CA . 54023 1 112 . 1 . 1 26 26 ASP CB C 13 41.157 . . . . . . . . 449 D CB . 54023 1 113 . 1 . 1 26 26 ASP N N 15 121.849 . . . . . . . . 449 D N . 54023 1 114 . 1 . 1 27 27 GLU H H 1 8.334 . . . . . . . . 450 E HN . 54023 1 115 . 1 . 1 27 27 GLU C C 13 174.451 . . . . . . . . 450 E C . 54023 1 116 . 1 . 1 27 27 GLU CA C 13 54.462 . . . . . . . . 450 E CA . 54023 1 117 . 1 . 1 27 27 GLU CB C 13 29.626 . . . . . . . . 450 E CB . 54023 1 118 . 1 . 1 27 27 GLU N N 15 122.469 . . . . . . . . 450 E N . 54023 1 119 . 1 . 1 29 29 ILE H H 1 8.452 . . . . . . . . 452 I HN . 54023 1 120 . 1 . 1 29 29 ILE C C 13 176.744 . . . . . . . . 452 I C . 54023 1 121 . 1 . 1 29 29 ILE CA C 13 61.238 . . . . . . . . 452 I CA . 54023 1 122 . 1 . 1 29 29 ILE CB C 13 38.635 . . . . . . . . 452 I CB . 54023 1 123 . 1 . 1 29 29 ILE N N 15 122.107 . . . . . . . . 452 I N . 54023 1 124 . 1 . 1 30 30 THR H H 1 8.394 . . . . . . . . 453 T HN . 54023 1 125 . 1 . 1 30 30 THR C C 13 174.157 . . . . . . . . 453 T C . 54023 1 126 . 1 . 1 30 30 THR CA C 13 61.871 . . . . . . . . 453 T CA . 54023 1 127 . 1 . 1 30 30 THR CB C 13 69.872 . . . . . . . . 453 T CB . 54023 1 128 . 1 . 1 30 30 THR N N 15 119.845 . . . . . . . . 453 T N . 54023 1 129 . 1 . 1 31 31 GLU H H 1 8.479 . . . . . . . . 454 E HN . 54023 1 130 . 1 . 1 31 31 GLU C C 13 175.381 . . . . . . . . 454 E C . 54023 1 131 . 1 . 1 31 31 GLU CA C 13 56.395 . . . . . . . . 454 E CA . 54023 1 132 . 1 . 1 31 31 GLU CB C 13 30.470 . . . . . . . . 454 E CB . 54023 1 133 . 1 . 1 31 31 GLU N N 15 123.976 . . . . . . . . 454 E N . 54023 1 134 . 1 . 1 32 32 ASN H H 1 8.448 . . . . . . . . 455 N HN . 54023 1 135 . 1 . 1 32 32 ASN C C 13 174.543 . . . . . . . . 455 N C . 54023 1 136 . 1 . 1 32 32 ASN CA C 13 52.986 . . . . . . . . 455 N CA . 54023 1 137 . 1 . 1 32 32 ASN CB C 13 39.335 . . . . . . . . 455 N CB . 54023 1 138 . 1 . 1 32 32 ASN N N 15 120.146 . . . . . . . . 455 N N . 54023 1 139 . 1 . 1 33 33 PHE H H 1 8.388 . . . . . . . . 456 F HN . 54023 1 140 . 1 . 1 33 33 PHE C C 13 174.878 . . . . . . . . 456 F C . 54023 1 141 . 1 . 1 33 33 PHE CA C 13 57.818 . . . . . . . . 456 F CA . 54023 1 142 . 1 . 1 33 33 PHE CB C 13 39.511 . . . . . . . . 456 F CB . 54023 1 143 . 1 . 1 33 33 PHE N N 15 121.645 . . . . . . . . 456 F N . 54023 1 144 . 1 . 1 34 34 GLU H H 1 7.970 . . . . . . . . 457 E HN . 54023 1 145 . 1 . 1 34 34 GLU C C 13 180.933 . . . . . . . . 457 E C . 54023 1 146 . 1 . 1 34 34 GLU CA C 13 58.078 . . . . . . . . 457 E CA . 54023 1 147 . 1 . 1 34 34 GLU CB C 13 31.020 . . . . . . . . 457 E CB . 54023 1 148 . 1 . 1 34 34 GLU N N 15 126.840 . . . . . . . . 457 E N . 54023 1 stop_ save_