BMRB Entry 53825

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53825

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Title:
1H-15N Chemical shifts of Dsk2 dHS1 (deletion construct) from S. cerevisiae
Deposition date:
2026-05-28
Original release date:
2026-06-29
Authors:
Acharya, Nirbhik; Castaneda, Carlos
Citation:

Citation: Niblo, Jessica; Acharya, Nirbhik; Watkins, Maxwell; Castaneda, Carlos; Sukenik, Shahar. "Intramolecular interactions between folded and disordered regions shape ubiquilin structure and function "  Adv. Sci. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 352 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pE-SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSLNIHIKSGQDKWEVNVAP ESTVLQFKEAINKANGIPVA NQRLIYSGKILKDDQTVESY HIQDGHSVHLVKSQPKPQTA SAAGANNATATGAAAGTGAT PNMSSGQSAGFNPGPDGGAL NNDSNNQDELLRMMENPIFQ SQMNEMLSNPQMLDFMIQSN PQLQAMGPQARQMLQSPMFR QMLTNPDMIRQSMQFARMMD PNAGMGSAGGAASAFPAPGG DAPEEGSNTNTTSSSNTGNN AGTNAGTNAGANTAANPFAS LLNPALNPFANAGNAASTGM PAFDPALLASMFQPPVQASQ AEDTRPPEERYEHQLRQLND MGFFDFDRNVAALRRSGGSV QGALDSLLNGDV

Data sets:
Data typeCount
15N chemical shifts273
1H chemical shifts273

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Dsk2 dHS11

Entities:

Entity 1, Dsk2 dHS1 352 residues - Formula weight is not available

The provided sequence is a Dsk2 variant (dHS1) lacking residues 114-134; author sequence numbering retains the wild-type scheme.

1   METSERLEUASNILEHISILELYSSERGLY
2   GLNASPLYSTRPGLUVALASNVALALAPRO
3   GLUSERTHRVALLEUGLNPHELYSGLUALA
4   ILEASNLYSALAASNGLYILEPROVALALA
5   ASNGLNARGLEUILETYRSERGLYLYSILE
6   LEULYSASPASPGLNTHRVALGLUSERTYR
7   HISILEGLNASPGLYHISSERVALHISLEU
8   VALLYSSERGLNPROLYSPROGLNTHRALA
9   SERALAALAGLYALAASNASNALATHRALA
10   THRGLYALAALAALAGLYTHRGLYALATHR
11   PROASNMETSERSERGLYGLNSERALAGLY
12   PHEASNPROGLYPROASPGLYGLYALALEU
13   ASNASNASPSERASNASNGLNASPGLULEU
14   LEUARGMETMETGLUASNPROILEPHEGLN
15   SERGLNMETASNGLUMETLEUSERASNPRO
16   GLNMETLEUASPPHEMETILEGLNSERASN
17   PROGLNLEUGLNALAMETGLYPROGLNALA
18   ARGGLNMETLEUGLNSERPROMETPHEARG
19   GLNMETLEUTHRASNPROASPMETILEARG
20   GLNSERMETGLNPHEALAARGMETMETASP
21   PROASNALAGLYMETGLYSERALAGLYGLY
22   ALAALASERALAPHEPROALAPROGLYGLY
23   ASPALAPROGLUGLUGLYSERASNTHRASN
24   THRTHRSERSERSERASNTHRGLYASNASN
25   ALAGLYTHRASNALAGLYTHRASNALAGLY
26   ALAASNTHRALAALAASNPROPHEALASER
27   LEULEUASNPROALALEUASNPROPHEALA
28   ASNALAGLYASNALAALASERTHRGLYMET
29   PROALAPHEASPPROALALEULEUALASER
30   METPHEGLNPROPROVALGLNALASERGLN
31   ALAGLUASPTHRARGPROPROGLUGLUARG
32   TYRGLUHISGLNLEUARGGLNLEUASNASP
33   METGLYPHEPHEASPPHEASPARGASNVAL
34   ALAALALEUARGARGSERGLYGLYSERVAL
35   GLNGLYALALEUASPSERLEULEUASNGLY
36   ASPVAL

Samples:

sample_1: Dsk2 dHS1, [U-99% 15N], 50 uM; sodium phosphate 20 mM; sodium azide 0.02%; EDTA 0.5 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

CcpNMR v2.5.1 - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

UNP P48510

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks