data_53825 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 53825 _Entry.Title ; 1H-15N Chemical shifts of Dsk2 dHS1 (deletion construct) from S. cerevisiae ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2026-05-28 _Entry.Accession_date 2026-05-28 _Entry.Last_release_date 2026-05-29 _Entry.Original_release_date 2026-05-29 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID . _Entry.Generated_software_label . _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details 'Peaks were assigned by transfer from previously deposited Dsk2 backbone assignments BMRB 53439' _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Nirbhik Acharya . . . 0000-0003-4591-5215 53825 2 Carlos Castaneda . A. . 0000-0001-9634-0867 53825 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 53825 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '15N chemical shifts' 273 53825 '1H chemical shifts' 273 53825 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2026-06-29 . original BMRB . 53825 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 53439 'Chemical shifts of Dsk2 from S. cerevisiae' 53825 BMRB 53824 '1H-15N Chemical shifts of Dsk2 I45A mutant from S. cerevisiae' 53825 BMRB 53826 '1H-15N Chemical shifts of Dsk2 dHS2 (deletion construct) from S. cerevisiae' 53825 BMRB 53827 '1H-15N Chemical shifts of Dsk2 dHS3 (deletion construct) from S. cerevisiae' 53825 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 53825 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID . _Citation.DOI 10.1002/advs.75904 _Citation.Full_citation . _Citation.Title ; Intramolecular interactions between folded and disordered regions shape ubiquilin structure and function ; _Citation.Status 'in press' _Citation.Type journal _Citation.Journal_abbrev 'Adv. Sci.' _Citation.Journal_name_full . _Citation.Journal_volume . _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first . _Citation.Page_last . _Citation.Year . _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Jessica Niblo . K. . . 53825 1 2 Nirbhik Acharya . . . . 53825 1 3 Maxwell Watkins . B. . . 53825 1 4 Carlos Castaneda . A. . . 53825 1 5 Shahar Sukenik . . . . 53825 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 53825 _Assembly.ID 1 _Assembly.Name 'Dsk2 dHS1' _Assembly.BMRB_code . _Assembly.Number_of_components 1 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states no _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass 37055 _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 'Dsk2 dHS1' 1 $entity_1 . . yes native no no . . . 53825 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 53825 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MSLNIHIKSGQDKWEVNVAP ESTVLQFKEAINKANGIPVA NQRLIYSGKILKDDQTVESY HIQDGHSVHLVKSQPKPQTA SAAGANNATATGAAAGTGAT PNMSSGQSAGFNPGPDGGAL NNDSNNQDELLRMMENPIFQ SQMNEMLSNPQMLDFMIQSN PQLQAMGPQARQMLQSPMFR QMLTNPDMIRQSMQFARMMD PNAGMGSAGGAASAFPAPGG DAPEEGSNTNTTSSSNTGNN AGTNAGTNAGANTAANPFAS LLNPALNPFANAGNAASTGM PAFDPALLASMFQPPVQASQ AEDTRPPEERYEHQLRQLND MGFFDFDRNVAALRRSGGSV QGALDSLLNGDV ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq ; 1,M 2,S 3,L 4,N 5,I 6,H 7,I 8,K 9,S 10,G 11,Q 12,D 13,K 14,W 15,E 16,V 17,N 18,V 19,A 20,P 21,E 22,S 23,T 24,V 25,L 26,Q 27,F 28,K 29,E 30,A 31,I 32,N 33,K 34,A 35,N 36,G 37,I 38,P 39,V 40,A 41,N 42,Q 43,R 44,L 45,I 46,Y 47,S 48,G 49,K 50,I 51,L 52,K 53,D 54,D 55,Q 56,T 57,V 58,E 59,S 60,Y 61,H 62,I 63,Q 64,D 65,G 66,H 67,S 68,V 69,H 70,L 71,V 72,K 73,S 74,Q 75,P 76,K 77,P 78,Q 79,T 80,A 81,S 82,A 83,A 84,G 85,A 86,N 87,N 88,A 89,T 90,A 91,T 92,G 93,A 94,A 95,A 96,G 97,T 98,G 99,A 100,T 101,P 102,N 103,M 104,S 105,S 106,G 107,Q 108,S 109,A 110,G 111,F 112,N 113,P 135,G 136,P 137,D 138,G 139,G 140,A 141,L 142,N 143,N 144,D 145,S 146,N 147,N 148,Q 149,D 150,E 151,L 152,L 153,R 154,M 155,M 156,E 157,N 158,P 159,I 160,F 161,Q 162,S 163,Q 164,M 165,N 166,E 167,M 168,L 169,S 170,N 171,P 172,Q 173,M 174,L 175,D 176,F 177,M 178,I 179,Q 180,S 181,N 182,P 183,Q 184,L 185,Q 186,A 187,M 188,G 189,P 190,Q 191,A 192,R 193,Q 194,M 195,L 196,Q 197,S 198,P 199,M 200,F 201,R 202,Q 203,M 204,L 205,T 206,N 207,P 208,D 209,M 210,I 211,R 212,Q 213,S 214,M 215,Q 216,F 217,A 218,R 219,M 220,M 221,D 222,P 223,N 224,A 225,G 226,M 227,G 228,S 229,A 230,G 231,G 232,A 233,A 234,S 235,A 236,F 237,P 238,A 239,P 240,G 241,G 242,D 243,A 244,P 245,E 246,E 247,G 248,S 249,N 250,T 251,N 252,T 253,T 254,S 255,S 256,S 257,N 258,T 259,G 260,N 261,N 262,A 263,G 264,T 265,N 266,A 267,G 268,T 269,N 270,A 271,G 272,A 273,N 274,T 275,A 276,A 277,N 278,P 279,F 280,A 281,S 282,L 283,L 284,N 285,P 286,A 287,L 288,N 289,P 290,F 291,A 292,N 293,A 294,G 295,N 296,A 297,A 298,S 299,T 300,G 301,M 302,P 303,A 304,F 305,D 306,P 307,A 308,L 309,L 310,A 311,S 312,M 313,F 314,Q 315,P 316,P 317,V 318,Q 319,A 320,S 321,Q 322,A 323,E 324,D 325,T 326,R 327,P 328,P 329,E 330,E 331,R 332,Y 333,E 334,H 335,Q 336,L 337,R 338,Q 339,L 340,N 341,D 342,M 343,G 344,F 345,F 346,D 347,F 348,D 349,R 350,N 351,V 352,A 353,A 354,L 355,R 356,R 357,S 358,G 359,G 360,S 361,V 362,Q 363,G 364,A 365,L 366,D 367,S 368,L 369,L 370,N 371,G 372,D 373,V ; _Entity.Polymer_author_seq_details 'The provided sequence is a Dsk2 variant (dHS1) lacking residues 114-134; author sequence numbering retains the wild-type scheme.' _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 352 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation 'Deletion of residues 114-134' _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 yes UNP P48510 . DSK2 . . . . . . . . . . . . . . 53825 1 stop_ loop_ _Entity_biological_function.Biological_function _Entity_biological_function.Entry_ID _Entity_biological_function.Entity_ID 'Proteasomal shuttle protein that interacts with ubiquitin, ubiquitin chains, and substrates' 53825 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 1 MET . 53825 1 2 2 SER . 53825 1 3 3 LEU . 53825 1 4 4 ASN . 53825 1 5 5 ILE . 53825 1 6 6 HIS . 53825 1 7 7 ILE . 53825 1 8 8 LYS . 53825 1 9 9 SER . 53825 1 10 10 GLY . 53825 1 11 11 GLN . 53825 1 12 12 ASP . 53825 1 13 13 LYS . 53825 1 14 14 TRP . 53825 1 15 15 GLU . 53825 1 16 16 VAL . 53825 1 17 17 ASN . 53825 1 18 18 VAL . 53825 1 19 19 ALA . 53825 1 20 20 PRO . 53825 1 21 21 GLU . 53825 1 22 22 SER . 53825 1 23 23 THR . 53825 1 24 24 VAL . 53825 1 25 25 LEU . 53825 1 26 26 GLN . 53825 1 27 27 PHE . 53825 1 28 28 LYS . 53825 1 29 29 GLU . 53825 1 30 30 ALA . 53825 1 31 31 ILE . 53825 1 32 32 ASN . 53825 1 33 33 LYS . 53825 1 34 34 ALA . 53825 1 35 35 ASN . 53825 1 36 36 GLY . 53825 1 37 37 ILE . 53825 1 38 38 PRO . 53825 1 39 39 VAL . 53825 1 40 40 ALA . 53825 1 41 41 ASN . 53825 1 42 42 GLN . 53825 1 43 43 ARG . 53825 1 44 44 LEU . 53825 1 45 45 ILE . 53825 1 46 46 TYR . 53825 1 47 47 SER . 53825 1 48 48 GLY . 53825 1 49 49 LYS . 53825 1 50 50 ILE . 53825 1 51 51 LEU . 53825 1 52 52 LYS . 53825 1 53 53 ASP . 53825 1 54 54 ASP . 53825 1 55 55 GLN . 53825 1 56 56 THR . 53825 1 57 57 VAL . 53825 1 58 58 GLU . 53825 1 59 59 SER . 53825 1 60 60 TYR . 53825 1 61 61 HIS . 53825 1 62 62 ILE . 53825 1 63 63 GLN . 53825 1 64 64 ASP . 53825 1 65 65 GLY . 53825 1 66 66 HIS . 53825 1 67 67 SER . 53825 1 68 68 VAL . 53825 1 69 69 HIS . 53825 1 70 70 LEU . 53825 1 71 71 VAL . 53825 1 72 72 LYS . 53825 1 73 73 SER . 53825 1 74 74 GLN . 53825 1 75 75 PRO . 53825 1 76 76 LYS . 53825 1 77 77 PRO . 53825 1 78 78 GLN . 53825 1 79 79 THR . 53825 1 80 80 ALA . 53825 1 81 81 SER . 53825 1 82 82 ALA . 53825 1 83 83 ALA . 53825 1 84 84 GLY . 53825 1 85 85 ALA . 53825 1 86 86 ASN . 53825 1 87 87 ASN . 53825 1 88 88 ALA . 53825 1 89 89 THR . 53825 1 90 90 ALA . 53825 1 91 91 THR . 53825 1 92 92 GLY . 53825 1 93 93 ALA . 53825 1 94 94 ALA . 53825 1 95 95 ALA . 53825 1 96 96 GLY . 53825 1 97 97 THR . 53825 1 98 98 GLY . 53825 1 99 99 ALA . 53825 1 100 100 THR . 53825 1 101 101 PRO . 53825 1 102 102 ASN . 53825 1 103 103 MET . 53825 1 104 104 SER . 53825 1 105 105 SER . 53825 1 106 106 GLY . 53825 1 107 107 GLN . 53825 1 108 108 SER . 53825 1 109 109 ALA . 53825 1 110 110 GLY . 53825 1 111 111 PHE . 53825 1 112 112 ASN . 53825 1 113 113 PRO . 53825 1 114 135 GLY . 53825 1 115 136 PRO . 53825 1 116 137 ASP . 53825 1 117 138 GLY . 53825 1 118 139 GLY . 53825 1 119 140 ALA . 53825 1 120 141 LEU . 53825 1 121 142 ASN . 53825 1 122 143 ASN . 53825 1 123 144 ASP . 53825 1 124 145 SER . 53825 1 125 146 ASN . 53825 1 126 147 ASN . 53825 1 127 148 GLN . 53825 1 128 149 ASP . 53825 1 129 150 GLU . 53825 1 130 151 LEU . 53825 1 131 152 LEU . 53825 1 132 153 ARG . 53825 1 133 154 MET . 53825 1 134 155 MET . 53825 1 135 156 GLU . 53825 1 136 157 ASN . 53825 1 137 158 PRO . 53825 1 138 159 ILE . 53825 1 139 160 PHE . 53825 1 140 161 GLN . 53825 1 141 162 SER . 53825 1 142 163 GLN . 53825 1 143 164 MET . 53825 1 144 165 ASN . 53825 1 145 166 GLU . 53825 1 146 167 MET . 53825 1 147 168 LEU . 53825 1 148 169 SER . 53825 1 149 170 ASN . 53825 1 150 171 PRO . 53825 1 151 172 GLN . 53825 1 152 173 MET . 53825 1 153 174 LEU . 53825 1 154 175 ASP . 53825 1 155 176 PHE . 53825 1 156 177 MET . 53825 1 157 178 ILE . 53825 1 158 179 GLN . 53825 1 159 180 SER . 53825 1 160 181 ASN . 53825 1 161 182 PRO . 53825 1 162 183 GLN . 53825 1 163 184 LEU . 53825 1 164 185 GLN . 53825 1 165 186 ALA . 53825 1 166 187 MET . 53825 1 167 188 GLY . 53825 1 168 189 PRO . 53825 1 169 190 GLN . 53825 1 170 191 ALA . 53825 1 171 192 ARG . 53825 1 172 193 GLN . 53825 1 173 194 MET . 53825 1 174 195 LEU . 53825 1 175 196 GLN . 53825 1 176 197 SER . 53825 1 177 198 PRO . 53825 1 178 199 MET . 53825 1 179 200 PHE . 53825 1 180 201 ARG . 53825 1 181 202 GLN . 53825 1 182 203 MET . 53825 1 183 204 LEU . 53825 1 184 205 THR . 53825 1 185 206 ASN . 53825 1 186 207 PRO . 53825 1 187 208 ASP . 53825 1 188 209 MET . 53825 1 189 210 ILE . 53825 1 190 211 ARG . 53825 1 191 212 GLN . 53825 1 192 213 SER . 53825 1 193 214 MET . 53825 1 194 215 GLN . 53825 1 195 216 PHE . 53825 1 196 217 ALA . 53825 1 197 218 ARG . 53825 1 198 219 MET . 53825 1 199 220 MET . 53825 1 200 221 ASP . 53825 1 201 222 PRO . 53825 1 202 223 ASN . 53825 1 203 224 ALA . 53825 1 204 225 GLY . 53825 1 205 226 MET . 53825 1 206 227 GLY . 53825 1 207 228 SER . 53825 1 208 229 ALA . 53825 1 209 230 GLY . 53825 1 210 231 GLY . 53825 1 211 232 ALA . 53825 1 212 233 ALA . 53825 1 213 234 SER . 53825 1 214 235 ALA . 53825 1 215 236 PHE . 53825 1 216 237 PRO . 53825 1 217 238 ALA . 53825 1 218 239 PRO . 53825 1 219 240 GLY . 53825 1 220 241 GLY . 53825 1 221 242 ASP . 53825 1 222 243 ALA . 53825 1 223 244 PRO . 53825 1 224 245 GLU . 53825 1 225 246 GLU . 53825 1 226 247 GLY . 53825 1 227 248 SER . 53825 1 228 249 ASN . 53825 1 229 250 THR . 53825 1 230 251 ASN . 53825 1 231 252 THR . 53825 1 232 253 THR . 53825 1 233 254 SER . 53825 1 234 255 SER . 53825 1 235 256 SER . 53825 1 236 257 ASN . 53825 1 237 258 THR . 53825 1 238 259 GLY . 53825 1 239 260 ASN . 53825 1 240 261 ASN . 53825 1 241 262 ALA . 53825 1 242 263 GLY . 53825 1 243 264 THR . 53825 1 244 265 ASN . 53825 1 245 266 ALA . 53825 1 246 267 GLY . 53825 1 247 268 THR . 53825 1 248 269 ASN . 53825 1 249 270 ALA . 53825 1 250 271 GLY . 53825 1 251 272 ALA . 53825 1 252 273 ASN . 53825 1 253 274 THR . 53825 1 254 275 ALA . 53825 1 255 276 ALA . 53825 1 256 277 ASN . 53825 1 257 278 PRO . 53825 1 258 279 PHE . 53825 1 259 280 ALA . 53825 1 260 281 SER . 53825 1 261 282 LEU . 53825 1 262 283 LEU . 53825 1 263 284 ASN . 53825 1 264 285 PRO . 53825 1 265 286 ALA . 53825 1 266 287 LEU . 53825 1 267 288 ASN . 53825 1 268 289 PRO . 53825 1 269 290 PHE . 53825 1 270 291 ALA . 53825 1 271 292 ASN . 53825 1 272 293 ALA . 53825 1 273 294 GLY . 53825 1 274 295 ASN . 53825 1 275 296 ALA . 53825 1 276 297 ALA . 53825 1 277 298 SER . 53825 1 278 299 THR . 53825 1 279 300 GLY . 53825 1 280 301 MET . 53825 1 281 302 PRO . 53825 1 282 303 ALA . 53825 1 283 304 PHE . 53825 1 284 305 ASP . 53825 1 285 306 PRO . 53825 1 286 307 ALA . 53825 1 287 308 LEU . 53825 1 288 309 LEU . 53825 1 289 310 ALA . 53825 1 290 311 SER . 53825 1 291 312 MET . 53825 1 292 313 PHE . 53825 1 293 314 GLN . 53825 1 294 315 PRO . 53825 1 295 316 PRO . 53825 1 296 317 VAL . 53825 1 297 318 GLN . 53825 1 298 319 ALA . 53825 1 299 320 SER . 53825 1 300 321 GLN . 53825 1 301 322 ALA . 53825 1 302 323 GLU . 53825 1 303 324 ASP . 53825 1 304 325 THR . 53825 1 305 326 ARG . 53825 1 306 327 PRO . 53825 1 307 328 PRO . 53825 1 308 329 GLU . 53825 1 309 330 GLU . 53825 1 310 331 ARG . 53825 1 311 332 TYR . 53825 1 312 333 GLU . 53825 1 313 334 HIS . 53825 1 314 335 GLN . 53825 1 315 336 LEU . 53825 1 316 337 ARG . 53825 1 317 338 GLN . 53825 1 318 339 LEU . 53825 1 319 340 ASN . 53825 1 320 341 ASP . 53825 1 321 342 MET . 53825 1 322 343 GLY . 53825 1 323 344 PHE . 53825 1 324 345 PHE . 53825 1 325 346 ASP . 53825 1 326 347 PHE . 53825 1 327 348 ASP . 53825 1 328 349 ARG . 53825 1 329 350 ASN . 53825 1 330 351 VAL . 53825 1 331 352 ALA . 53825 1 332 353 ALA . 53825 1 333 354 LEU . 53825 1 334 355 ARG . 53825 1 335 356 ARG . 53825 1 336 357 SER . 53825 1 337 358 GLY . 53825 1 338 359 GLY . 53825 1 339 360 SER . 53825 1 340 361 VAL . 53825 1 341 362 GLN . 53825 1 342 363 GLY . 53825 1 343 364 ALA . 53825 1 344 365 LEU . 53825 1 345 366 ASP . 53825 1 346 367 SER . 53825 1 347 368 LEU . 53825 1 348 369 LEU . 53825 1 349 370 ASN . 53825 1 350 371 GLY . 53825 1 351 372 ASP . 53825 1 352 373 VAL . 53825 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 53825 1 . SER 2 2 53825 1 . LEU 3 3 53825 1 . ASN 4 4 53825 1 . ILE 5 5 53825 1 . HIS 6 6 53825 1 . ILE 7 7 53825 1 . LYS 8 8 53825 1 . SER 9 9 53825 1 . GLY 10 10 53825 1 . GLN 11 11 53825 1 . ASP 12 12 53825 1 . LYS 13 13 53825 1 . TRP 14 14 53825 1 . GLU 15 15 53825 1 . VAL 16 16 53825 1 . ASN 17 17 53825 1 . VAL 18 18 53825 1 . ALA 19 19 53825 1 . PRO 20 20 53825 1 . GLU 21 21 53825 1 . SER 22 22 53825 1 . THR 23 23 53825 1 . VAL 24 24 53825 1 . LEU 25 25 53825 1 . GLN 26 26 53825 1 . PHE 27 27 53825 1 . LYS 28 28 53825 1 . GLU 29 29 53825 1 . ALA 30 30 53825 1 . ILE 31 31 53825 1 . ASN 32 32 53825 1 . LYS 33 33 53825 1 . ALA 34 34 53825 1 . ASN 35 35 53825 1 . GLY 36 36 53825 1 . ILE 37 37 53825 1 . PRO 38 38 53825 1 . VAL 39 39 53825 1 . ALA 40 40 53825 1 . ASN 41 41 53825 1 . GLN 42 42 53825 1 . ARG 43 43 53825 1 . LEU 44 44 53825 1 . ILE 45 45 53825 1 . TYR 46 46 53825 1 . SER 47 47 53825 1 . GLY 48 48 53825 1 . LYS 49 49 53825 1 . ILE 50 50 53825 1 . LEU 51 51 53825 1 . LYS 52 52 53825 1 . ASP 53 53 53825 1 . ASP 54 54 53825 1 . GLN 55 55 53825 1 . THR 56 56 53825 1 . VAL 57 57 53825 1 . GLU 58 58 53825 1 . SER 59 59 53825 1 . TYR 60 60 53825 1 . HIS 61 61 53825 1 . ILE 62 62 53825 1 . GLN 63 63 53825 1 . ASP 64 64 53825 1 . GLY 65 65 53825 1 . HIS 66 66 53825 1 . SER 67 67 53825 1 . VAL 68 68 53825 1 . HIS 69 69 53825 1 . LEU 70 70 53825 1 . VAL 71 71 53825 1 . LYS 72 72 53825 1 . SER 73 73 53825 1 . GLN 74 74 53825 1 . PRO 75 75 53825 1 . LYS 76 76 53825 1 . PRO 77 77 53825 1 . GLN 78 78 53825 1 . THR 79 79 53825 1 . ALA 80 80 53825 1 . SER 81 81 53825 1 . ALA 82 82 53825 1 . ALA 83 83 53825 1 . GLY 84 84 53825 1 . ALA 85 85 53825 1 . ASN 86 86 53825 1 . ASN 87 87 53825 1 . ALA 88 88 53825 1 . THR 89 89 53825 1 . ALA 90 90 53825 1 . THR 91 91 53825 1 . GLY 92 92 53825 1 . ALA 93 93 53825 1 . ALA 94 94 53825 1 . ALA 95 95 53825 1 . GLY 96 96 53825 1 . THR 97 97 53825 1 . GLY 98 98 53825 1 . ALA 99 99 53825 1 . THR 100 100 53825 1 . PRO 101 101 53825 1 . ASN 102 102 53825 1 . MET 103 103 53825 1 . SER 104 104 53825 1 . SER 105 105 53825 1 . GLY 106 106 53825 1 . GLN 107 107 53825 1 . SER 108 108 53825 1 . ALA 109 109 53825 1 . GLY 110 110 53825 1 . PHE 111 111 53825 1 . ASN 112 112 53825 1 . PRO 113 113 53825 1 . GLY 114 114 53825 1 . PRO 115 115 53825 1 . ASP 116 116 53825 1 . GLY 117 117 53825 1 . GLY 118 118 53825 1 . ALA 119 119 53825 1 . LEU 120 120 53825 1 . ASN 121 121 53825 1 . ASN 122 122 53825 1 . ASP 123 123 53825 1 . SER 124 124 53825 1 . ASN 125 125 53825 1 . ASN 126 126 53825 1 . GLN 127 127 53825 1 . ASP 128 128 53825 1 . GLU 129 129 53825 1 . LEU 130 130 53825 1 . LEU 131 131 53825 1 . ARG 132 132 53825 1 . MET 133 133 53825 1 . MET 134 134 53825 1 . GLU 135 135 53825 1 . ASN 136 136 53825 1 . PRO 137 137 53825 1 . ILE 138 138 53825 1 . PHE 139 139 53825 1 . GLN 140 140 53825 1 . SER 141 141 53825 1 . GLN 142 142 53825 1 . MET 143 143 53825 1 . ASN 144 144 53825 1 . GLU 145 145 53825 1 . MET 146 146 53825 1 . LEU 147 147 53825 1 . SER 148 148 53825 1 . ASN 149 149 53825 1 . PRO 150 150 53825 1 . GLN 151 151 53825 1 . MET 152 152 53825 1 . LEU 153 153 53825 1 . ASP 154 154 53825 1 . PHE 155 155 53825 1 . MET 156 156 53825 1 . ILE 157 157 53825 1 . GLN 158 158 53825 1 . SER 159 159 53825 1 . ASN 160 160 53825 1 . PRO 161 161 53825 1 . GLN 162 162 53825 1 . LEU 163 163 53825 1 . GLN 164 164 53825 1 . ALA 165 165 53825 1 . MET 166 166 53825 1 . GLY 167 167 53825 1 . PRO 168 168 53825 1 . GLN 169 169 53825 1 . ALA 170 170 53825 1 . ARG 171 171 53825 1 . GLN 172 172 53825 1 . MET 173 173 53825 1 . LEU 174 174 53825 1 . GLN 175 175 53825 1 . SER 176 176 53825 1 . PRO 177 177 53825 1 . MET 178 178 53825 1 . PHE 179 179 53825 1 . ARG 180 180 53825 1 . GLN 181 181 53825 1 . MET 182 182 53825 1 . LEU 183 183 53825 1 . THR 184 184 53825 1 . ASN 185 185 53825 1 . PRO 186 186 53825 1 . ASP 187 187 53825 1 . MET 188 188 53825 1 . ILE 189 189 53825 1 . ARG 190 190 53825 1 . GLN 191 191 53825 1 . SER 192 192 53825 1 . MET 193 193 53825 1 . GLN 194 194 53825 1 . PHE 195 195 53825 1 . ALA 196 196 53825 1 . ARG 197 197 53825 1 . MET 198 198 53825 1 . MET 199 199 53825 1 . ASP 200 200 53825 1 . PRO 201 201 53825 1 . ASN 202 202 53825 1 . ALA 203 203 53825 1 . GLY 204 204 53825 1 . MET 205 205 53825 1 . GLY 206 206 53825 1 . SER 207 207 53825 1 . ALA 208 208 53825 1 . GLY 209 209 53825 1 . GLY 210 210 53825 1 . ALA 211 211 53825 1 . ALA 212 212 53825 1 . SER 213 213 53825 1 . ALA 214 214 53825 1 . PHE 215 215 53825 1 . PRO 216 216 53825 1 . ALA 217 217 53825 1 . PRO 218 218 53825 1 . GLY 219 219 53825 1 . GLY 220 220 53825 1 . ASP 221 221 53825 1 . ALA 222 222 53825 1 . PRO 223 223 53825 1 . GLU 224 224 53825 1 . GLU 225 225 53825 1 . GLY 226 226 53825 1 . SER 227 227 53825 1 . ASN 228 228 53825 1 . THR 229 229 53825 1 . ASN 230 230 53825 1 . THR 231 231 53825 1 . THR 232 232 53825 1 . SER 233 233 53825 1 . SER 234 234 53825 1 . SER 235 235 53825 1 . ASN 236 236 53825 1 . THR 237 237 53825 1 . GLY 238 238 53825 1 . ASN 239 239 53825 1 . ASN 240 240 53825 1 . ALA 241 241 53825 1 . GLY 242 242 53825 1 . THR 243 243 53825 1 . ASN 244 244 53825 1 . ALA 245 245 53825 1 . GLY 246 246 53825 1 . THR 247 247 53825 1 . ASN 248 248 53825 1 . ALA 249 249 53825 1 . GLY 250 250 53825 1 . ALA 251 251 53825 1 . ASN 252 252 53825 1 . THR 253 253 53825 1 . ALA 254 254 53825 1 . ALA 255 255 53825 1 . ASN 256 256 53825 1 . PRO 257 257 53825 1 . PHE 258 258 53825 1 . ALA 259 259 53825 1 . SER 260 260 53825 1 . LEU 261 261 53825 1 . LEU 262 262 53825 1 . ASN 263 263 53825 1 . PRO 264 264 53825 1 . ALA 265 265 53825 1 . LEU 266 266 53825 1 . ASN 267 267 53825 1 . PRO 268 268 53825 1 . PHE 269 269 53825 1 . ALA 270 270 53825 1 . ASN 271 271 53825 1 . ALA 272 272 53825 1 . GLY 273 273 53825 1 . ASN 274 274 53825 1 . ALA 275 275 53825 1 . ALA 276 276 53825 1 . SER 277 277 53825 1 . THR 278 278 53825 1 . GLY 279 279 53825 1 . MET 280 280 53825 1 . PRO 281 281 53825 1 . ALA 282 282 53825 1 . PHE 283 283 53825 1 . ASP 284 284 53825 1 . PRO 285 285 53825 1 . ALA 286 286 53825 1 . LEU 287 287 53825 1 . LEU 288 288 53825 1 . ALA 289 289 53825 1 . SER 290 290 53825 1 . MET 291 291 53825 1 . PHE 292 292 53825 1 . GLN 293 293 53825 1 . PRO 294 294 53825 1 . PRO 295 295 53825 1 . VAL 296 296 53825 1 . GLN 297 297 53825 1 . ALA 298 298 53825 1 . SER 299 299 53825 1 . GLN 300 300 53825 1 . ALA 301 301 53825 1 . GLU 302 302 53825 1 . ASP 303 303 53825 1 . THR 304 304 53825 1 . ARG 305 305 53825 1 . PRO 306 306 53825 1 . PRO 307 307 53825 1 . GLU 308 308 53825 1 . GLU 309 309 53825 1 . ARG 310 310 53825 1 . TYR 311 311 53825 1 . GLU 312 312 53825 1 . HIS 313 313 53825 1 . GLN 314 314 53825 1 . LEU 315 315 53825 1 . ARG 316 316 53825 1 . GLN 317 317 53825 1 . LEU 318 318 53825 1 . ASN 319 319 53825 1 . ASP 320 320 53825 1 . MET 321 321 53825 1 . GLY 322 322 53825 1 . PHE 323 323 53825 1 . PHE 324 324 53825 1 . ASP 325 325 53825 1 . PHE 326 326 53825 1 . ASP 327 327 53825 1 . ARG 328 328 53825 1 . ASN 329 329 53825 1 . VAL 330 330 53825 1 . ALA 331 331 53825 1 . ALA 332 332 53825 1 . LEU 333 333 53825 1 . ARG 334 334 53825 1 . ARG 335 335 53825 1 . SER 336 336 53825 1 . GLY 337 337 53825 1 . GLY 338 338 53825 1 . SER 339 339 53825 1 . VAL 340 340 53825 1 . GLN 341 341 53825 1 . GLY 342 342 53825 1 . ALA 343 343 53825 1 . LEU 344 344 53825 1 . ASP 345 345 53825 1 . SER 346 346 53825 1 . LEU 347 347 53825 1 . LEU 348 348 53825 1 . ASN 349 349 53825 1 . GLY 350 350 53825 1 . ASP 351 351 53825 1 . VAL 352 352 53825 1 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 53825 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 4932 organism . 'Saccharomyces cerevisiae' "baker's yeast" . . Eukaryota Fungi Saccharomyces cerevisiae . . . . . . . . . . . . . 53825 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 53825 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . pE-SUMO . . . 53825 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 53825 _Sample.ID 1 _Sample.Name 15N_Dsk2_dHS1 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number . _Sample.Solvent_system '95% H2O/5% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'Dsk2 dHS1' '[U-99% 15N]' . . 1 $entity_1 . . 50 . . uM . . . . 53825 1 2 'sodium phosphate' 'natural abundance' . . . . . . 20 . . mM . . . . 53825 1 3 'sodium azide' 'natural abundance' . . . . . . 0.02 . . % . . . . 53825 1 4 EDTA 'natural abundance' . . . . . . 0.5 . . mM . . . . 53825 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 53825 _Sample_condition_list.ID 1 _Sample_condition_list.Name 'Buffer conditions' _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 20 . mM 53825 1 pH 6.8 . pH 53825 1 pressure 1 . atm 53825 1 temperature 298.15 . K 53825 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 53825 _Software.ID 1 _Software.Type . _Software.Name NMRPipe _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID processing . 53825 1 stop_ save_ save_software_2 _Software.Sf_category software _Software.Sf_framecode software_2 _Software.Entry_ID 53825 _Software.ID 2 _Software.Type . _Software.Name CcpNMR _Software.Version 2.5.1 _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'data analysis' . 53825 2 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 53825 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name 'Bruker Avance 800 MHz' _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model Avance _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 800 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 53825 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-15N TROSY' no no no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 53825 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chem_shift_reference_1 _Chem_shift_reference.Entry_ID 53825 _Chem_shift_reference.ID 1 _Chem_shift_reference.Name 'Reference Set Name' _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID H 1 DSS 'methyl protons' . . . . ppm 0.00 internal direct 1 . . . . . 53825 1 N 15 DSS 'methyl protons' . . . . ppm 0.00 na indirect 0.101329118 . . . . . 53825 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_1 _Assigned_chem_shift_list.Entry_ID 53825 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Name 'Dsk2 dHS1' _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 '2D 1H-15N TROSY' . . . 53825 1 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 1 $software_1 . . 53825 1 2 $software_2 . . 53825 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 . 1 3 3 LEU H H 1 8.850 . . . . . . . . 3 L HN . 53825 1 2 . 1 . 1 3 3 LEU N N 15 122.920 . . . . . . . . 3 L N . 53825 1 3 . 1 . 1 4 4 ASN H H 1 8.993 . . . . . . . . 4 N HN . 53825 1 4 . 1 . 1 4 4 ASN N N 15 122.474 . . . . . . . . 4 N N . 53825 1 5 . 1 . 1 5 5 ILE H H 1 8.825 . . . . . . . . 5 I HN . 53825 1 6 . 1 . 1 5 5 ILE N N 15 125.972 . . . . . . . . 5 I N . 53825 1 7 . 1 . 1 6 6 HIS H H 1 8.597 . . . . . . . . 6 H HN . 53825 1 8 . 1 . 1 6 6 HIS N N 15 125.275 . . . . . . . . 6 H N . 53825 1 9 . 1 . 1 7 7 ILE H H 1 9.495 . . . . . . . . 7 I HN . 53825 1 10 . 1 . 1 7 7 ILE N N 15 125.340 . . . . . . . . 7 I N . 53825 1 11 . 1 . 1 8 8 LYS H H 1 8.579 . . . . . . . . 8 K HN . 53825 1 12 . 1 . 1 8 8 LYS N N 15 125.567 . . . . . . . . 8 K N . 53825 1 13 . 1 . 1 9 9 SER H H 1 8.258 . . . . . . . . 9 S HN . 53825 1 14 . 1 . 1 9 9 SER N N 15 117.841 . . . . . . . . 9 S N . 53825 1 15 . 1 . 1 11 11 GLN H H 1 9.142 . . . . . . . . 11 Q HN . 53825 1 16 . 1 . 1 11 11 GLN N N 15 126.522 . . . . . . . . 11 Q N . 53825 1 17 . 1 . 1 12 12 ASP H H 1 8.070 . . . . . . . . 12 D HN . 53825 1 18 . 1 . 1 12 12 ASP N N 15 120.770 . . . . . . . . 12 D N . 53825 1 19 . 1 . 1 13 13 LYS H H 1 7.597 . . . . . . . . 13 K HN . 53825 1 20 . 1 . 1 13 13 LYS N N 15 119.686 . . . . . . . . 13 K N . 53825 1 21 . 1 . 1 14 14 TRP H H 1 9.070 . . . . . . . . 14 W HN . 53825 1 22 . 1 . 1 14 14 TRP N N 15 121.465 . . . . . . . . 14 W N . 53825 1 23 . 1 . 1 15 15 GLU H H 1 8.731 . . . . . . . . 15 E HN . 53825 1 24 . 1 . 1 15 15 GLU N N 15 122.311 . . . . . . . . 15 E N . 53825 1 25 . 1 . 1 16 16 VAL H H 1 8.754 . . . . . . . . 16 V HN . 53825 1 26 . 1 . 1 16 16 VAL N N 15 119.477 . . . . . . . . 16 V N . 53825 1 27 . 1 . 1 17 17 ASN H H 1 8.451 . . . . . . . . 17 N HN . 53825 1 28 . 1 . 1 17 17 ASN N N 15 123.425 . . . . . . . . 17 N N . 53825 1 29 . 1 . 1 18 18 VAL H H 1 8.822 . . . . . . . . 18 V HN . 53825 1 30 . 1 . 1 18 18 VAL N N 15 119.219 . . . . . . . . 18 V N . 53825 1 31 . 1 . 1 19 19 ALA H H 1 8.426 . . . . . . . . 19 A HN . 53825 1 32 . 1 . 1 19 19 ALA N N 15 128.260 . . . . . . . . 19 A N . 53825 1 33 . 1 . 1 21 21 GLU H H 1 8.852 . . . . . . . . 21 E HN . 53825 1 34 . 1 . 1 21 21 GLU N N 15 112.699 . . . . . . . . 21 E N . 53825 1 35 . 1 . 1 22 22 SER H H 1 7.692 . . . . . . . . 22 S HN . 53825 1 36 . 1 . 1 22 22 SER N N 15 117.154 . . . . . . . . 22 S N . 53825 1 37 . 1 . 1 23 23 THR H H 1 8.789 . . . . . . . . 23 T HN . 53825 1 38 . 1 . 1 23 23 THR N N 15 113.281 . . . . . . . . 23 T N . 53825 1 39 . 1 . 1 24 24 VAL H H 1 9.122 . . . . . . . . 24 V HN . 53825 1 40 . 1 . 1 24 24 VAL N N 15 123.893 . . . . . . . . 24 V N . 53825 1 41 . 1 . 1 25 25 LEU H H 1 8.380 . . . . . . . . 25 L HN . 53825 1 42 . 1 . 1 25 25 LEU N N 15 122.705 . . . . . . . . 25 L N . 53825 1 43 . 1 . 1 26 26 GLN H H 1 7.780 . . . . . . . . 26 Q HN . 53825 1 44 . 1 . 1 26 26 GLN N N 15 118.144 . . . . . . . . 26 Q N . 53825 1 45 . 1 . 1 27 27 PHE H H 1 8.733 . . . . . . . . 27 F HN . 53825 1 46 . 1 . 1 27 27 PHE N N 15 124.690 . . . . . . . . 27 F N . 53825 1 47 . 1 . 1 28 28 LYS H H 1 8.873 . . . . . . . . 28 K HN . 53825 1 48 . 1 . 1 28 28 LYS N N 15 120.292 . . . . . . . . 28 K N . 53825 1 49 . 1 . 1 29 29 GLU H H 1 8.360 . . . . . . . . 29 E HN . 53825 1 50 . 1 . 1 29 29 GLU N N 15 121.508 . . . . . . . . 29 E N . 53825 1 51 . 1 . 1 30 30 ALA H H 1 7.984 . . . . . . . . 30 A HN . 53825 1 52 . 1 . 1 30 30 ALA N N 15 124.285 . . . . . . . . 30 A N . 53825 1 53 . 1 . 1 31 31 ILE H H 1 8.590 . . . . . . . . 31 I HN . 53825 1 54 . 1 . 1 31 31 ILE N N 15 121.934 . . . . . . . . 31 I N . 53825 1 55 . 1 . 1 32 32 ASN H H 1 8.514 . . . . . . . . 32 N HN . 53825 1 56 . 1 . 1 32 32 ASN N N 15 117.712 . . . . . . . . 32 N N . 53825 1 57 . 1 . 1 33 33 LYS H H 1 7.794 . . . . . . . . 33 K HN . 53825 1 58 . 1 . 1 33 33 LYS N N 15 119.637 . . . . . . . . 33 K N . 53825 1 59 . 1 . 1 34 34 ALA H H 1 7.325 . . . . . . . . 34 A HN . 53825 1 60 . 1 . 1 34 34 ALA N N 15 119.902 . . . . . . . . 34 A N . 53825 1 61 . 1 . 1 35 35 ASN H H 1 8.120 . . . . . . . . 35 N HN . 53825 1 62 . 1 . 1 35 35 ASN N N 15 111.498 . . . . . . . . 35 N N . 53825 1 63 . 1 . 1 36 36 GLY H H 1 8.702 . . . . . . . . 36 G HN . 53825 1 64 . 1 . 1 36 36 GLY N N 15 110.055 . . . . . . . . 36 G N . 53825 1 65 . 1 . 1 37 37 ILE H H 1 7.923 . . . . . . . . 37 I HN . 53825 1 66 . 1 . 1 37 37 ILE N N 15 124.305 . . . . . . . . 37 I N . 53825 1 67 . 1 . 1 39 39 VAL H H 1 8.734 . . . . . . . . 39 V HN . 53825 1 68 . 1 . 1 39 39 VAL N N 15 124.840 . . . . . . . . 39 V N . 53825 1 69 . 1 . 1 40 40 ALA H H 1 8.676 . . . . . . . . 40 A HN . 53825 1 70 . 1 . 1 40 40 ALA N N 15 118.608 . . . . . . . . 40 A N . 53825 1 71 . 1 . 1 41 41 ASN H H 1 7.700 . . . . . . . . 41 N HN . 53825 1 72 . 1 . 1 41 41 ASN N N 15 113.686 . . . . . . . . 41 N N . 53825 1 73 . 1 . 1 42 42 GLN H H 1 7.306 . . . . . . . . 42 Q HN . 53825 1 74 . 1 . 1 42 42 GLN N N 15 118.371 . . . . . . . . 42 Q N . 53825 1 75 . 1 . 1 43 43 ARG H H 1 8.101 . . . . . . . . 43 R HN . 53825 1 76 . 1 . 1 43 43 ARG N N 15 124.296 . . . . . . . . 43 R N . 53825 1 77 . 1 . 1 45 45 ILE H H 1 9.323 . . . . . . . . 45 I HN . 53825 1 78 . 1 . 1 45 45 ILE N N 15 122.401 . . . . . . . . 45 I N . 53825 1 79 . 1 . 1 46 46 TYR H H 1 8.959 . . . . . . . . 46 Y HN . 53825 1 80 . 1 . 1 46 46 TYR N N 15 126.557 . . . . . . . . 46 Y N . 53825 1 81 . 1 . 1 48 48 GLY H H 1 8.388 . . . . . . . . 48 G HN . 53825 1 82 . 1 . 1 48 48 GLY N N 15 103.984 . . . . . . . . 48 G N . 53825 1 83 . 1 . 1 51 51 LEU H H 1 8.511 . . . . . . . . 51 L HN . 53825 1 84 . 1 . 1 51 51 LEU N N 15 126.851 . . . . . . . . 51 L N . 53825 1 85 . 1 . 1 52 52 LYS H H 1 8.450 . . . . . . . . 52 K HN . 53825 1 86 . 1 . 1 52 52 LYS N N 15 124.302 . . . . . . . . 52 K N . 53825 1 87 . 1 . 1 53 53 ASP H H 1 8.154 . . . . . . . . 53 D HN . 53825 1 88 . 1 . 1 53 53 ASP N N 15 120.965 . . . . . . . . 53 D N . 53825 1 89 . 1 . 1 54 54 ASP H H 1 8.347 . . . . . . . . 54 D HN . 53825 1 90 . 1 . 1 54 54 ASP N N 15 113.366 . . . . . . . . 54 D N . 53825 1 91 . 1 . 1 55 55 GLN H H 1 7.809 . . . . . . . . 55 Q HN . 53825 1 92 . 1 . 1 55 55 GLN N N 15 119.432 . . . . . . . . 55 Q N . 53825 1 93 . 1 . 1 56 56 THR H H 1 8.323 . . . . . . . . 56 T HN . 53825 1 94 . 1 . 1 56 56 THR N N 15 108.403 . . . . . . . . 56 T N . 53825 1 95 . 1 . 1 57 57 VAL H H 1 8.797 . . . . . . . . 57 V HN . 53825 1 96 . 1 . 1 57 57 VAL N N 15 123.631 . . . . . . . . 57 V N . 53825 1 97 . 1 . 1 58 58 GLU H H 1 8.684 . . . . . . . . 58 E HN . 53825 1 98 . 1 . 1 58 58 GLU N N 15 117.267 . . . . . . . . 58 E N . 53825 1 99 . 1 . 1 59 59 SER H H 1 7.904 . . . . . . . . 59 S HN . 53825 1 100 . 1 . 1 59 59 SER N N 15 118.654 . . . . . . . . 59 S N . 53825 1 101 . 1 . 1 60 60 TYR H H 1 7.224 . . . . . . . . 60 Y HN . 53825 1 102 . 1 . 1 60 60 TYR N N 15 118.991 . . . . . . . . 60 Y N . 53825 1 103 . 1 . 1 61 61 HIS H H 1 8.158 . . . . . . . . 61 H HN . 53825 1 104 . 1 . 1 61 61 HIS N N 15 113.288 . . . . . . . . 61 H N . 53825 1 105 . 1 . 1 62 62 ILE H H 1 6.576 . . . . . . . . 62 I HN . 53825 1 106 . 1 . 1 62 62 ILE N N 15 118.605 . . . . . . . . 62 I N . 53825 1 107 . 1 . 1 63 63 GLN H H 1 6.135 . . . . . . . . 63 Q HN . 53825 1 108 . 1 . 1 63 63 GLN N N 15 123.614 . . . . . . . . 63 Q N . 53825 1 109 . 1 . 1 65 65 GLY H H 1 9.285 . . . . . . . . 65 G HN . 53825 1 110 . 1 . 1 65 65 GLY N N 15 113.896 . . . . . . . . 65 G N . 53825 1 111 . 1 . 1 66 66 HIS H H 1 8.124 . . . . . . . . 66 H HN . 53825 1 112 . 1 . 1 66 66 HIS N N 15 120.167 . . . . . . . . 66 H N . 53825 1 113 . 1 . 1 67 67 SER H H 1 8.657 . . . . . . . . 67 S HN . 53825 1 114 . 1 . 1 67 67 SER N N 15 113.070 . . . . . . . . 67 S N . 53825 1 115 . 1 . 1 68 68 VAL H H 1 9.082 . . . . . . . . 68 V HN . 53825 1 116 . 1 . 1 68 68 VAL N N 15 122.982 . . . . . . . . 68 V N . 53825 1 117 . 1 . 1 69 69 HIS H H 1 9.026 . . . . . . . . 69 H HN . 53825 1 118 . 1 . 1 69 69 HIS N N 15 125.933 . . . . . . . . 69 H N . 53825 1 119 . 1 . 1 70 70 LEU H H 1 8.986 . . . . . . . . 70 L HN . 53825 1 120 . 1 . 1 70 70 LEU N N 15 125.224 . . . . . . . . 70 L N . 53825 1 121 . 1 . 1 71 71 VAL H H 1 9.432 . . . . . . . . 71 V HN . 53825 1 122 . 1 . 1 71 71 VAL N N 15 130.035 . . . . . . . . 71 V N . 53825 1 123 . 1 . 1 72 72 LYS H H 1 8.674 . . . . . . . . 72 K HN . 53825 1 124 . 1 . 1 72 72 LYS N N 15 127.109 . . . . . . . . 72 K N . 53825 1 125 . 1 . 1 73 73 SER H H 1 8.681 . . . . . . . . 73 S HN . 53825 1 126 . 1 . 1 73 73 SER N N 15 120.173 . . . . . . . . 73 S N . 53825 1 127 . 1 . 1 74 74 GLN H H 1 8.476 . . . . . . . . 74 Q HN . 53825 1 128 . 1 . 1 74 74 GLN N N 15 122.878 . . . . . . . . 74 Q N . 53825 1 129 . 1 . 1 76 76 LYS H H 1 8.423 . . . . . . . . 76 K HN . 53825 1 130 . 1 . 1 76 76 LYS N N 15 123.078 . . . . . . . . 76 K N . 53825 1 131 . 1 . 1 78 78 GLN H H 1 8.579 . . . . . . . . 78 Q HN . 53825 1 132 . 1 . 1 78 78 GLN N N 15 121.184 . . . . . . . . 78 Q N . 53825 1 133 . 1 . 1 79 79 THR H H 1 8.143 . . . . . . . . 79 T HN . 53825 1 134 . 1 . 1 79 79 THR N N 15 115.482 . . . . . . . . 79 T N . 53825 1 135 . 1 . 1 80 80 ALA H H 1 8.407 . . . . . . . . 80 A HN . 53825 1 136 . 1 . 1 80 80 ALA N N 15 126.664 . . . . . . . . 80 A N . 53825 1 137 . 1 . 1 81 81 SER H H 1 8.284 . . . . . . . . 81 S HN . 53825 1 138 . 1 . 1 81 81 SER N N 15 115.230 . . . . . . . . 81 S N . 53825 1 139 . 1 . 1 82 82 ALA H H 1 8.332 . . . . . . . . 82 A HN . 53825 1 140 . 1 . 1 82 82 ALA N N 15 126.369 . . . . . . . . 82 A N . 53825 1 141 . 1 . 1 83 83 ALA H H 1 8.232 . . . . . . . . 83 A HN . 53825 1 142 . 1 . 1 83 83 ALA N N 15 123.191 . . . . . . . . 83 A N . 53825 1 143 . 1 . 1 84 84 GLY H H 1 8.283 . . . . . . . . 84 G HN . 53825 1 144 . 1 . 1 84 84 GLY N N 15 108.136 . . . . . . . . 84 G N . 53825 1 145 . 1 . 1 85 85 ALA H H 1 8.103 . . . . . . . . 85 A HN . 53825 1 146 . 1 . 1 85 85 ALA N N 15 123.561 . . . . . . . . 85 A N . 53825 1 147 . 1 . 1 86 86 ASN H H 1 8.432 . . . . . . . . 86 N HN . 53825 1 148 . 1 . 1 86 86 ASN N N 15 117.598 . . . . . . . . 86 N N . 53825 1 149 . 1 . 1 87 87 ASN H H 1 8.324 . . . . . . . . 87 N HN . 53825 1 150 . 1 . 1 87 87 ASN N N 15 119.202 . . . . . . . . 87 N N . 53825 1 151 . 1 . 1 88 88 ALA H H 1 8.261 . . . . . . . . 88 A HN . 53825 1 152 . 1 . 1 88 88 ALA N N 15 124.130 . . . . . . . . 88 A N . 53825 1 153 . 1 . 1 89 89 THR H H 1 8.079 . . . . . . . . 89 T HN . 53825 1 154 . 1 . 1 89 89 THR N N 15 112.782 . . . . . . . . 89 T N . 53825 1 155 . 1 . 1 90 90 ALA H H 1 8.285 . . . . . . . . 90 A HN . 53825 1 156 . 1 . 1 90 90 ALA N N 15 126.428 . . . . . . . . 90 A N . 53825 1 157 . 1 . 1 91 91 THR H H 1 8.117 . . . . . . . . 91 T HN . 53825 1 158 . 1 . 1 91 91 THR N N 15 112.694 . . . . . . . . 91 T N . 53825 1 159 . 1 . 1 92 92 GLY H H 1 8.347 . . . . . . . . 92 G HN . 53825 1 160 . 1 . 1 92 92 GLY N N 15 111.172 . . . . . . . . 92 G N . 53825 1 161 . 1 . 1 93 93 ALA H H 1 8.147 . . . . . . . . 93 A HN . 53825 1 162 . 1 . 1 93 93 ALA N N 15 124.038 . . . . . . . . 93 A N . 53825 1 163 . 1 . 1 94 94 ALA H H 1 8.263 . . . . . . . . 94 A HN . 53825 1 164 . 1 . 1 94 94 ALA N N 15 123.171 . . . . . . . . 94 A N . 53825 1 165 . 1 . 1 95 95 ALA H H 1 8.220 . . . . . . . . 95 A HN . 53825 1 166 . 1 . 1 95 95 ALA N N 15 123.263 . . . . . . . . 95 A N . 53825 1 167 . 1 . 1 96 96 GLY H H 1 8.338 . . . . . . . . 96 G HN . 53825 1 168 . 1 . 1 96 96 GLY N N 15 107.970 . . . . . . . . 96 G N . 53825 1 169 . 1 . 1 97 97 THR H H 1 8.091 . . . . . . . . 97 T HN . 53825 1 170 . 1 . 1 97 97 THR N N 15 112.851 . . . . . . . . 97 T N . 53825 1 171 . 1 . 1 98 98 GLY H H 1 8.479 . . . . . . . . 98 G HN . 53825 1 172 . 1 . 1 98 98 GLY N N 15 111.328 . . . . . . . . 98 G N . 53825 1 173 . 1 . 1 99 99 ALA H H 1 8.119 . . . . . . . . 99 A HN . 53825 1 174 . 1 . 1 99 99 ALA N N 15 123.547 . . . . . . . . 99 A N . 53825 1 175 . 1 . 1 100 100 THR H H 1 8.191 . . . . . . . . 100 T HN . 53825 1 176 . 1 . 1 100 100 THR N N 15 115.850 . . . . . . . . 100 T N . 53825 1 177 . 1 . 1 102 102 ASN H H 1 8.507 . . . . . . . . 102 N HN . 53825 1 178 . 1 . 1 102 102 ASN N N 15 118.528 . . . . . . . . 102 N N . 53825 1 179 . 1 . 1 103 103 MET H H 1 8.352 . . . . . . . . 103 M HN . 53825 1 180 . 1 . 1 103 103 MET N N 15 121.154 . . . . . . . . 103 M N . 53825 1 181 . 1 . 1 104 104 SER H H 1 8.369 . . . . . . . . 104 S HN . 53825 1 182 . 1 . 1 104 104 SER N N 15 116.892 . . . . . . . . 104 S N . 53825 1 183 . 1 . 1 105 105 SER H H 1 8.358 . . . . . . . . 105 S HN . 53825 1 184 . 1 . 1 105 105 SER N N 15 117.680 . . . . . . . . 105 S N . 53825 1 185 . 1 . 1 106 106 GLY H H 1 8.418 . . . . . . . . 106 G HN . 53825 1 186 . 1 . 1 106 106 GLY N N 15 110.639 . . . . . . . . 106 G N . 53825 1 187 . 1 . 1 107 107 GLN H H 1 8.208 . . . . . . . . 107 Q HN . 53825 1 188 . 1 . 1 107 107 GLN N N 15 119.868 . . . . . . . . 107 Q N . 53825 1 189 . 1 . 1 108 108 SER H H 1 8.385 . . . . . . . . 108 S HN . 53825 1 190 . 1 . 1 108 108 SER N N 15 117.082 . . . . . . . . 108 S N . 53825 1 191 . 1 . 1 109 109 ALA H H 1 8.351 . . . . . . . . 109 A HN . 53825 1 192 . 1 . 1 109 109 ALA N N 15 125.928 . . . . . . . . 109 A N . 53825 1 193 . 1 . 1 110 110 GLY H H 1 8.270 . . . . . . . . 110 G HN . 53825 1 194 . 1 . 1 110 110 GLY N N 15 107.748 . . . . . . . . 110 G N . 53825 1 195 . 1 . 1 112 112 ASN H H 1 8.378 . . . . . . . . 112 N HN . 53825 1 196 . 1 . 1 112 112 ASN N N 15 122.811 . . . . . . . . 112 N N . 53825 1 197 . 1 . 1 114 114 GLY H H 1 8.126 . . . . . . . . 135 G HN . 53825 1 198 . 1 . 1 114 114 GLY N N 15 108.564 . . . . . . . . 135 G N . 53825 1 199 . 1 . 1 116 116 ASP H H 1 8.394 . . . . . . . . 137 D HN . 53825 1 200 . 1 . 1 116 116 ASP N N 15 118.863 . . . . . . . . 137 D N . 53825 1 201 . 1 . 1 117 117 GLY H H 1 8.312 . . . . . . . . 138 G HN . 53825 1 202 . 1 . 1 117 117 GLY N N 15 109.504 . . . . . . . . 138 G N . 53825 1 203 . 1 . 1 118 118 GLY H H 1 8.273 . . . . . . . . 139 G HN . 53825 1 204 . 1 . 1 118 118 GLY N N 15 108.804 . . . . . . . . 139 G N . 53825 1 205 . 1 . 1 120 120 LEU H H 1 8.226 . . . . . . . . 141 L HN . 53825 1 206 . 1 . 1 120 120 LEU N N 15 120.907 . . . . . . . . 141 L N . 53825 1 207 . 1 . 1 121 121 ASN H H 1 8.351 . . . . . . . . 142 N HN . 53825 1 208 . 1 . 1 121 121 ASN N N 15 119.082 . . . . . . . . 142 N N . 53825 1 209 . 1 . 1 122 122 ASN H H 1 8.415 . . . . . . . . 143 N HN . 53825 1 210 . 1 . 1 122 122 ASN N N 15 119.676 . . . . . . . . 143 N N . 53825 1 211 . 1 . 1 123 123 ASP H H 1 8.369 . . . . . . . . 144 D HN . 53825 1 212 . 1 . 1 123 123 ASP N N 15 120.928 . . . . . . . . 144 D N . 53825 1 213 . 1 . 1 124 124 SER H H 1 8.283 . . . . . . . . 145 S HN . 53825 1 214 . 1 . 1 124 124 SER N N 15 116.115 . . . . . . . . 145 S N . 53825 1 215 . 1 . 1 125 125 ASN H H 1 8.429 . . . . . . . . 146 N HN . 53825 1 216 . 1 . 1 125 125 ASN N N 15 120.462 . . . . . . . . 146 N N . 53825 1 217 . 1 . 1 128 128 ASP H H 1 8.259 . . . . . . . . 149 D HN . 53825 1 218 . 1 . 1 128 128 ASP N N 15 119.997 . . . . . . . . 149 D N . 53825 1 219 . 1 . 1 130 130 LEU H H 1 7.983 . . . . . . . . 151 L HN . 53825 1 220 . 1 . 1 130 130 LEU N N 15 120.928 . . . . . . . . 151 L N . 53825 1 221 . 1 . 1 131 131 LEU H H 1 7.931 . . . . . . . . 152 L HN . 53825 1 222 . 1 . 1 131 131 LEU N N 15 118.806 . . . . . . . . 152 L N . 53825 1 223 . 1 . 1 132 132 ARG H H 1 7.801 . . . . . . . . 153 R HN . 53825 1 224 . 1 . 1 132 132 ARG N N 15 117.588 . . . . . . . . 153 R N . 53825 1 225 . 1 . 1 133 133 MET H H 1 7.849 . . . . . . . . 154 M HN . 53825 1 226 . 1 . 1 133 133 MET N N 15 119.872 . . . . . . . . 154 M N . 53825 1 227 . 1 . 1 134 134 MET H H 1 7.783 . . . . . . . . 155 M HN . 53825 1 228 . 1 . 1 134 134 MET N N 15 115.404 . . . . . . . . 155 M N . 53825 1 229 . 1 . 1 135 135 GLU H H 1 7.542 . . . . . . . . 156 E HN . 53825 1 230 . 1 . 1 135 135 GLU N N 15 115.840 . . . . . . . . 156 E N . 53825 1 231 . 1 . 1 136 136 ASN H H 1 7.746 . . . . . . . . 157 N HN . 53825 1 232 . 1 . 1 136 136 ASN N N 15 121.187 . . . . . . . . 157 N N . 53825 1 233 . 1 . 1 139 139 PHE H H 1 7.514 . . . . . . . . 160 F HN . 53825 1 234 . 1 . 1 139 139 PHE N N 15 121.963 . . . . . . . . 160 F N . 53825 1 235 . 1 . 1 140 140 GLN H H 1 8.276 . . . . . . . . 161 Q HN . 53825 1 236 . 1 . 1 140 140 GLN N N 15 117.283 . . . . . . . . 161 Q N . 53825 1 237 . 1 . 1 141 141 SER H H 1 8.047 . . . . . . . . 162 S HN . 53825 1 238 . 1 . 1 141 141 SER N N 15 114.128 . . . . . . . . 162 S N . 53825 1 239 . 1 . 1 142 142 GLN H H 1 7.975 . . . . . . . . 163 Q HN . 53825 1 240 . 1 . 1 142 142 GLN N N 15 121.819 . . . . . . . . 163 Q N . 53825 1 241 . 1 . 1 144 144 ASN H H 1 8.113 . . . . . . . . 165 N HN . 53825 1 242 . 1 . 1 144 144 ASN N N 15 117.146 . . . . . . . . 165 N N . 53825 1 243 . 1 . 1 145 145 GLU H H 1 7.999 . . . . . . . . 166 E HN . 53825 1 244 . 1 . 1 145 145 GLU N N 15 120.337 . . . . . . . . 166 E N . 53825 1 245 . 1 . 1 154 154 ASP H H 1 7.981 . . . . . . . . 175 D HN . 53825 1 246 . 1 . 1 154 154 ASP N N 15 117.215 . . . . . . . . 175 D N . 53825 1 247 . 1 . 1 155 155 PHE H H 1 7.825 . . . . . . . . 176 F HN . 53825 1 248 . 1 . 1 155 155 PHE N N 15 119.782 . . . . . . . . 176 F N . 53825 1 249 . 1 . 1 158 158 GLN H H 1 7.855 . . . . . . . . 179 Q HN . 53825 1 250 . 1 . 1 158 158 GLN N N 15 115.445 . . . . . . . . 179 Q N . 53825 1 251 . 1 . 1 159 159 SER H H 1 7.554 . . . . . . . . 180 S HN . 53825 1 252 . 1 . 1 159 159 SER N N 15 110.953 . . . . . . . . 180 S N . 53825 1 253 . 1 . 1 160 160 ASN H H 1 7.509 . . . . . . . . 181 N HN . 53825 1 254 . 1 . 1 160 160 ASN N N 15 120.559 . . . . . . . . 181 N N . 53825 1 255 . 1 . 1 162 162 GLN H H 1 8.416 . . . . . . . . 183 Q HN . 53825 1 256 . 1 . 1 162 162 GLN N N 15 117.151 . . . . . . . . 183 Q N . 53825 1 257 . 1 . 1 164 164 GLN H H 1 8.094 . . . . . . . . 185 Q HN . 53825 1 258 . 1 . 1 164 164 GLN N N 15 119.689 . . . . . . . . 185 Q N . 53825 1 259 . 1 . 1 165 165 ALA H H 1 7.653 . . . . . . . . 186 A HN . 53825 1 260 . 1 . 1 165 165 ALA N N 15 119.539 . . . . . . . . 186 A N . 53825 1 261 . 1 . 1 166 166 MET H H 1 7.488 . . . . . . . . 187 M HN . 53825 1 262 . 1 . 1 166 166 MET N N 15 115.961 . . . . . . . . 187 M N . 53825 1 263 . 1 . 1 167 167 GLY H H 1 7.790 . . . . . . . . 188 G HN . 53825 1 264 . 1 . 1 167 167 GLY N N 15 107.395 . . . . . . . . 188 G N . 53825 1 265 . 1 . 1 169 169 GLN H H 1 8.346 . . . . . . . . 190 Q HN . 53825 1 266 . 1 . 1 169 169 GLN N N 15 117.381 . . . . . . . . 190 Q N . 53825 1 267 . 1 . 1 170 170 ALA H H 1 7.925 . . . . . . . . 191 A HN . 53825 1 268 . 1 . 1 170 170 ALA N N 15 122.285 . . . . . . . . 191 A N . 53825 1 269 . 1 . 1 171 171 ARG H H 1 7.818 . . . . . . . . 192 R HN . 53825 1 270 . 1 . 1 171 171 ARG N N 15 115.758 . . . . . . . . 192 R N . 53825 1 271 . 1 . 1 172 172 GLN H H 1 7.501 . . . . . . . . 193 Q HN . 53825 1 272 . 1 . 1 172 172 GLN N N 15 116.312 . . . . . . . . 193 Q N . 53825 1 273 . 1 . 1 175 175 GLN H H 1 7.293 . . . . . . . . 196 Q HN . 53825 1 274 . 1 . 1 175 175 GLN N N 15 112.569 . . . . . . . . 196 Q N . 53825 1 275 . 1 . 1 176 176 SER H H 1 7.703 . . . . . . . . 197 S HN . 53825 1 276 . 1 . 1 176 176 SER N N 15 117.169 . . . . . . . . 197 S N . 53825 1 277 . 1 . 1 178 178 MET H H 1 8.138 . . . . . . . . 199 M HN . 53825 1 278 . 1 . 1 178 178 MET N N 15 115.848 . . . . . . . . 199 M N . 53825 1 279 . 1 . 1 179 179 PHE H H 1 7.668 . . . . . . . . 200 F HN . 53825 1 280 . 1 . 1 179 179 PHE N N 15 119.950 . . . . . . . . 200 F N . 53825 1 281 . 1 . 1 180 180 ARG H H 1 7.894 . . . . . . . . 201 R HN . 53825 1 282 . 1 . 1 180 180 ARG N N 15 117.304 . . . . . . . . 201 R N . 53825 1 283 . 1 . 1 185 185 ASN H H 1 7.665 . . . . . . . . 206 N HN . 53825 1 284 . 1 . 1 185 185 ASN N N 15 120.247 . . . . . . . . 206 N N . 53825 1 285 . 1 . 1 187 187 ASP H H 1 8.228 . . . . . . . . 208 D HN . 53825 1 286 . 1 . 1 187 187 ASP N N 15 117.579 . . . . . . . . 208 D N . 53825 1 287 . 1 . 1 196 196 ALA H H 1 8.074 . . . . . . . . 217 A HN . 53825 1 288 . 1 . 1 196 196 ALA N N 15 122.014 . . . . . . . . 217 A N . 53825 1 289 . 1 . 1 202 202 ASN H H 1 8.483 . . . . . . . . 223 N HN . 53825 1 290 . 1 . 1 202 202 ASN N N 15 116.564 . . . . . . . . 223 N N . 53825 1 291 . 1 . 1 203 203 ALA H H 1 7.818 . . . . . . . . 224 A HN . 53825 1 292 . 1 . 1 203 203 ALA N N 15 123.511 . . . . . . . . 224 A N . 53825 1 293 . 1 . 1 204 204 GLY H H 1 8.366 . . . . . . . . 225 G HN . 53825 1 294 . 1 . 1 204 204 GLY N N 15 107.566 . . . . . . . . 225 G N . 53825 1 295 . 1 . 1 205 205 MET H H 1 8.142 . . . . . . . . 226 M HN . 53825 1 296 . 1 . 1 205 205 MET N N 15 119.530 . . . . . . . . 226 M N . 53825 1 297 . 1 . 1 206 206 GLY H H 1 8.468 . . . . . . . . 227 G HN . 53825 1 298 . 1 . 1 206 206 GLY N N 15 109.823 . . . . . . . . 227 G N . 53825 1 299 . 1 . 1 207 207 SER H H 1 8.222 . . . . . . . . 228 S HN . 53825 1 300 . 1 . 1 207 207 SER N N 15 115.710 . . . . . . . . 228 S N . 53825 1 301 . 1 . 1 208 208 ALA H H 1 8.415 . . . . . . . . 229 A HN . 53825 1 302 . 1 . 1 208 208 ALA N N 15 125.967 . . . . . . . . 229 A N . 53825 1 303 . 1 . 1 209 209 GLY H H 1 8.329 . . . . . . . . 230 G HN . 53825 1 304 . 1 . 1 209 209 GLY N N 15 107.956 . . . . . . . . 230 G N . 53825 1 305 . 1 . 1 210 210 GLY H H 1 8.196 . . . . . . . . 231 G HN . 53825 1 306 . 1 . 1 210 210 GLY N N 15 108.617 . . . . . . . . 231 G N . 53825 1 307 . 1 . 1 211 211 ALA H H 1 8.199 . . . . . . . . 232 A HN . 53825 1 308 . 1 . 1 211 211 ALA N N 15 123.933 . . . . . . . . 232 A N . 53825 1 309 . 1 . 1 212 212 ALA H H 1 8.286 . . . . . . . . 233 A HN . 53825 1 310 . 1 . 1 212 212 ALA N N 15 122.978 . . . . . . . . 233 A N . 53825 1 311 . 1 . 1 213 213 SER H H 1 8.112 . . . . . . . . 234 S HN . 53825 1 312 . 1 . 1 213 213 SER N N 15 114.542 . . . . . . . . 234 S N . 53825 1 313 . 1 . 1 214 214 ALA H H 1 8.143 . . . . . . . . 235 A HN . 53825 1 314 . 1 . 1 214 214 ALA N N 15 125.562 . . . . . . . . 235 A N . 53825 1 315 . 1 . 1 215 215 PHE H H 1 8.079 . . . . . . . . 236 F HN . 53825 1 316 . 1 . 1 215 215 PHE N N 15 119.964 . . . . . . . . 236 F N . 53825 1 317 . 1 . 1 217 217 ALA H H 1 8.380 . . . . . . . . 238 A HN . 53825 1 318 . 1 . 1 217 217 ALA N N 15 125.982 . . . . . . . . 238 A N . 53825 1 319 . 1 . 1 219 219 GLY H H 1 8.491 . . . . . . . . 240 G HN . 53825 1 320 . 1 . 1 219 219 GLY N N 15 109.436 . . . . . . . . 240 G N . 53825 1 321 . 1 . 1 220 220 GLY H H 1 8.247 . . . . . . . . 241 G HN . 53825 1 322 . 1 . 1 220 220 GLY N N 15 108.834 . . . . . . . . 241 G N . 53825 1 323 . 1 . 1 221 221 ASP H H 1 8.319 . . . . . . . . 242 D HN . 53825 1 324 . 1 . 1 221 221 ASP N N 15 120.272 . . . . . . . . 242 D N . 53825 1 325 . 1 . 1 222 222 ALA H H 1 8.235 . . . . . . . . 243 A HN . 53825 1 326 . 1 . 1 222 222 ALA N N 15 125.261 . . . . . . . . 243 A N . 53825 1 327 . 1 . 1 224 224 GLU H H 1 8.582 . . . . . . . . 245 E HN . 53825 1 328 . 1 . 1 224 224 GLU N N 15 121.400 . . . . . . . . 245 E N . 53825 1 329 . 1 . 1 225 225 GLU H H 1 8.528 . . . . . . . . 246 E HN . 53825 1 330 . 1 . 1 225 225 GLU N N 15 122.783 . . . . . . . . 246 E N . 53825 1 331 . 1 . 1 226 226 GLY H H 1 8.493 . . . . . . . . 247 G HN . 53825 1 332 . 1 . 1 226 226 GLY N N 15 110.400 . . . . . . . . 247 G N . 53825 1 333 . 1 . 1 227 227 SER H H 1 8.207 . . . . . . . . 248 S HN . 53825 1 334 . 1 . 1 227 227 SER N N 15 115.418 . . . . . . . . 248 S N . 53825 1 335 . 1 . 1 228 228 ASN H H 1 8.555 . . . . . . . . 249 N HN . 53825 1 336 . 1 . 1 228 228 ASN N N 15 120.799 . . . . . . . . 249 N N . 53825 1 337 . 1 . 1 229 229 THR H H 1 8.142 . . . . . . . . 250 T HN . 53825 1 338 . 1 . 1 229 229 THR N N 15 113.901 . . . . . . . . 250 T N . 53825 1 339 . 1 . 1 230 230 ASN H H 1 8.492 . . . . . . . . 251 N HN . 53825 1 340 . 1 . 1 230 230 ASN N N 15 121.113 . . . . . . . . 251 N N . 53825 1 341 . 1 . 1 231 231 THR H H 1 8.191 . . . . . . . . 252 T HN . 53825 1 342 . 1 . 1 231 231 THR N N 15 114.341 . . . . . . . . 252 T N . 53825 1 343 . 1 . 1 232 232 THR H H 1 8.208 . . . . . . . . 253 T HN . 53825 1 344 . 1 . 1 232 232 THR N N 15 115.962 . . . . . . . . 253 T N . 53825 1 345 . 1 . 1 233 233 SER H H 1 8.360 . . . . . . . . 254 S HN . 53825 1 346 . 1 . 1 233 233 SER N N 15 118.150 . . . . . . . . 254 S N . 53825 1 347 . 1 . 1 234 234 SER H H 1 8.394 . . . . . . . . 255 S HN . 53825 1 348 . 1 . 1 234 234 SER N N 15 117.943 . . . . . . . . 255 S N . 53825 1 349 . 1 . 1 235 235 SER H H 1 8.337 . . . . . . . . 256 S HN . 53825 1 350 . 1 . 1 235 235 SER N N 15 117.395 . . . . . . . . 256 S N . 53825 1 351 . 1 . 1 236 236 ASN H H 1 8.461 . . . . . . . . 257 N HN . 53825 1 352 . 1 . 1 236 236 ASN N N 15 120.915 . . . . . . . . 257 N N . 53825 1 353 . 1 . 1 237 237 THR H H 1 8.140 . . . . . . . . 258 T HN . 53825 1 354 . 1 . 1 237 237 THR N N 15 113.544 . . . . . . . . 258 T N . 53825 1 355 . 1 . 1 238 238 GLY H H 1 8.407 . . . . . . . . 259 G HN . 53825 1 356 . 1 . 1 238 238 GLY N N 15 110.804 . . . . . . . . 259 G N . 53825 1 357 . 1 . 1 239 239 ASN H H 1 8.317 . . . . . . . . 260 N HN . 53825 1 358 . 1 . 1 239 239 ASN N N 15 118.728 . . . . . . . . 260 N N . 53825 1 359 . 1 . 1 240 240 ASN H H 1 8.456 . . . . . . . . 261 N HN . 53825 1 360 . 1 . 1 240 240 ASN N N 15 119.432 . . . . . . . . 261 N N . 53825 1 361 . 1 . 1 241 241 ALA H H 1 8.273 . . . . . . . . 262 A HN . 53825 1 362 . 1 . 1 241 241 ALA N N 15 124.123 . . . . . . . . 262 A N . 53825 1 363 . 1 . 1 242 242 GLY H H 1 8.376 . . . . . . . . 263 G HN . 53825 1 364 . 1 . 1 242 242 GLY N N 15 107.824 . . . . . . . . 263 G N . 53825 1 365 . 1 . 1 243 243 THR H H 1 8.028 . . . . . . . . 264 T HN . 53825 1 366 . 1 . 1 243 243 THR N N 15 113.373 . . . . . . . . 264 T N . 53825 1 367 . 1 . 1 244 244 ASN H H 1 8.502 . . . . . . . . 265 N HN . 53825 1 368 . 1 . 1 244 244 ASN N N 15 121.100 . . . . . . . . 265 N N . 53825 1 369 . 1 . 1 245 245 ALA H H 1 8.282 . . . . . . . . 266 A HN . 53825 1 370 . 1 . 1 245 245 ALA N N 15 124.528 . . . . . . . . 266 A N . 53825 1 371 . 1 . 1 246 246 GLY H H 1 8.383 . . . . . . . . 267 G HN . 53825 1 372 . 1 . 1 246 246 GLY N N 15 107.821 . . . . . . . . 267 G N . 53825 1 373 . 1 . 1 247 247 THR H H 1 8.033 . . . . . . . . 268 T HN . 53825 1 374 . 1 . 1 247 247 THR N N 15 113.369 . . . . . . . . 268 T N . 53825 1 375 . 1 . 1 248 248 ASN H H 1 8.486 . . . . . . . . 269 N HN . 53825 1 376 . 1 . 1 248 248 ASN N N 15 121.110 . . . . . . . . 269 N N . 53825 1 377 . 1 . 1 249 249 ALA H H 1 8.285 . . . . . . . . 270 A HN . 53825 1 378 . 1 . 1 249 249 ALA N N 15 124.625 . . . . . . . . 270 A N . 53825 1 379 . 1 . 1 250 250 GLY H H 1 8.349 . . . . . . . . 271 G HN . 53825 1 380 . 1 . 1 250 250 GLY N N 15 107.963 . . . . . . . . 271 G N . 53825 1 381 . 1 . 1 251 251 ALA H H 1 8.080 . . . . . . . . 272 A HN . 53825 1 382 . 1 . 1 251 251 ALA N N 15 123.494 . . . . . . . . 272 A N . 53825 1 383 . 1 . 1 252 252 ASN H H 1 8.439 . . . . . . . . 273 N HN . 53825 1 384 . 1 . 1 252 252 ASN N N 15 117.806 . . . . . . . . 273 N N . 53825 1 385 . 1 . 1 253 253 THR H H 1 8.053 . . . . . . . . 274 T HN . 53825 1 386 . 1 . 1 253 253 THR N N 15 114.391 . . . . . . . . 274 T N . 53825 1 387 . 1 . 1 254 254 ALA H H 1 8.224 . . . . . . . . 275 A HN . 53825 1 388 . 1 . 1 254 254 ALA N N 15 125.878 . . . . . . . . 275 A N . 53825 1 389 . 1 . 1 255 255 ALA H H 1 8.112 . . . . . . . . 276 A HN . 53825 1 390 . 1 . 1 255 255 ALA N N 15 122.643 . . . . . . . . 276 A N . 53825 1 391 . 1 . 1 256 256 ASN H H 1 8.250 . . . . . . . . 277 N HN . 53825 1 392 . 1 . 1 256 256 ASN N N 15 118.217 . . . . . . . . 277 N N . 53825 1 393 . 1 . 1 258 258 PHE H H 1 8.033 . . . . . . . . 279 F HN . 53825 1 394 . 1 . 1 258 258 PHE N N 15 117.500 . . . . . . . . 279 F N . 53825 1 395 . 1 . 1 259 259 ALA H H 1 7.723 . . . . . . . . 280 A HN . 53825 1 396 . 1 . 1 259 259 ALA N N 15 123.281 . . . . . . . . 280 A N . 53825 1 397 . 1 . 1 260 260 SER H H 1 8.131 . . . . . . . . 281 S HN . 53825 1 398 . 1 . 1 260 260 SER N N 15 113.365 . . . . . . . . 281 S N . 53825 1 399 . 1 . 1 261 261 LEU H H 1 7.933 . . . . . . . . 282 L HN . 53825 1 400 . 1 . 1 261 261 LEU N N 15 122.370 . . . . . . . . 282 L N . 53825 1 401 . 1 . 1 262 262 LEU H H 1 7.839 . . . . . . . . 283 L HN . 53825 1 402 . 1 . 1 262 262 LEU N N 15 119.786 . . . . . . . . 283 L N . 53825 1 403 . 1 . 1 263 263 ASN H H 1 8.049 . . . . . . . . 284 N HN . 53825 1 404 . 1 . 1 263 263 ASN N N 15 119.464 . . . . . . . . 284 N N . 53825 1 405 . 1 . 1 265 265 ALA H H 1 8.067 . . . . . . . . 286 A HN . 53825 1 406 . 1 . 1 265 265 ALA N N 15 121.057 . . . . . . . . 286 A N . 53825 1 407 . 1 . 1 266 266 LEU H H 1 7.755 . . . . . . . . 287 L HN . 53825 1 408 . 1 . 1 266 266 LEU N N 15 118.739 . . . . . . . . 287 L N . 53825 1 409 . 1 . 1 267 267 ASN H H 1 8.050 . . . . . . . . 288 N HN . 53825 1 410 . 1 . 1 267 267 ASN N N 15 119.121 . . . . . . . . 288 N N . 53825 1 411 . 1 . 1 269 269 PHE H H 1 7.951 . . . . . . . . 290 F HN . 53825 1 412 . 1 . 1 269 269 PHE N N 15 117.514 . . . . . . . . 290 F N . 53825 1 413 . 1 . 1 270 270 ALA H H 1 7.696 . . . . . . . . 291 A HN . 53825 1 414 . 1 . 1 270 270 ALA N N 15 123.488 . . . . . . . . 291 A N . 53825 1 415 . 1 . 1 271 271 ASN H H 1 8.263 . . . . . . . . 292 N HN . 53825 1 416 . 1 . 1 271 271 ASN N N 15 117.193 . . . . . . . . 292 N N . 53825 1 417 . 1 . 1 272 272 ALA H H 1 8.202 . . . . . . . . 293 A HN . 53825 1 418 . 1 . 1 272 272 ALA N N 15 124.073 . . . . . . . . 293 A N . 53825 1 419 . 1 . 1 273 273 GLY H H 1 8.373 . . . . . . . . 294 G HN . 53825 1 420 . 1 . 1 273 273 GLY N N 15 107.566 . . . . . . . . 294 G N . 53825 1 421 . 1 . 1 274 274 ASN H H 1 8.199 . . . . . . . . 295 N HN . 53825 1 422 . 1 . 1 274 274 ASN N N 15 118.671 . . . . . . . . 295 N N . 53825 1 423 . 1 . 1 275 275 ALA H H 1 8.279 . . . . . . . . 296 A HN . 53825 1 424 . 1 . 1 275 275 ALA N N 15 124.441 . . . . . . . . 296 A N . 53825 1 425 . 1 . 1 276 276 ALA H H 1 8.231 . . . . . . . . 297 A HN . 53825 1 426 . 1 . 1 276 276 ALA N N 15 122.366 . . . . . . . . 297 A N . 53825 1 427 . 1 . 1 277 277 SER H H 1 8.195 . . . . . . . . 298 S HN . 53825 1 428 . 1 . 1 277 277 SER N N 15 114.456 . . . . . . . . 298 S N . 53825 1 429 . 1 . 1 278 278 THR H H 1 8.087 . . . . . . . . 299 T HN . 53825 1 430 . 1 . 1 278 278 THR N N 15 114.654 . . . . . . . . 299 T N . 53825 1 431 . 1 . 1 279 279 GLY H H 1 8.332 . . . . . . . . 300 G HN . 53825 1 432 . 1 . 1 279 279 GLY N N 15 110.718 . . . . . . . . 300 G N . 53825 1 433 . 1 . 1 280 280 MET H H 1 8.092 . . . . . . . . 301 M HN . 53825 1 434 . 1 . 1 280 280 MET N N 15 120.796 . . . . . . . . 301 M N . 53825 1 435 . 1 . 1 282 282 ALA H H 1 8.261 . . . . . . . . 303 A HN . 53825 1 436 . 1 . 1 282 282 ALA N N 15 123.442 . . . . . . . . 303 A N . 53825 1 437 . 1 . 1 283 283 PHE H H 1 8.006 . . . . . . . . 304 F HN . 53825 1 438 . 1 . 1 283 283 PHE N N 15 118.789 . . . . . . . . 304 F N . 53825 1 439 . 1 . 1 284 284 ASP H H 1 8.039 . . . . . . . . 305 D HN . 53825 1 440 . 1 . 1 284 284 ASP N N 15 124.352 . . . . . . . . 305 D N . 53825 1 441 . 1 . 1 286 286 ALA H H 1 8.364 . . . . . . . . 307 A HN . 53825 1 442 . 1 . 1 286 286 ALA N N 15 121.154 . . . . . . . . 307 A N . 53825 1 443 . 1 . 1 287 287 LEU H H 1 7.727 . . . . . . . . 308 L HN . 53825 1 444 . 1 . 1 287 287 LEU N N 15 119.266 . . . . . . . . 308 L N . 53825 1 445 . 1 . 1 288 288 LEU H H 1 7.711 . . . . . . . . 309 L HN . 53825 1 446 . 1 . 1 288 288 LEU N N 15 119.617 . . . . . . . . 309 L N . 53825 1 447 . 1 . 1 290 290 SER H H 1 7.838 . . . . . . . . 311 S HN . 53825 1 448 . 1 . 1 290 290 SER N N 15 112.540 . . . . . . . . 311 S N . 53825 1 449 . 1 . 1 291 291 MET H H 1 7.830 . . . . . . . . 312 M HN . 53825 1 450 . 1 . 1 291 291 MET N N 15 120.335 . . . . . . . . 312 M N . 53825 1 451 . 1 . 1 293 293 GLN H H 1 7.918 . . . . . . . . 314 Q HN . 53825 1 452 . 1 . 1 293 293 GLN N N 15 122.237 . . . . . . . . 314 Q N . 53825 1 453 . 1 . 1 296 296 VAL H H 1 8.206 . . . . . . . . 317 V HN . 53825 1 454 . 1 . 1 296 296 VAL N N 15 120.568 . . . . . . . . 317 V N . 53825 1 455 . 1 . 1 297 297 GLN H H 1 8.467 . . . . . . . . 318 Q HN . 53825 1 456 . 1 . 1 297 297 GLN N N 15 124.474 . . . . . . . . 318 Q N . 53825 1 457 . 1 . 1 298 298 ALA H H 1 8.436 . . . . . . . . 319 A HN . 53825 1 458 . 1 . 1 298 298 ALA N N 15 126.243 . . . . . . . . 319 A N . 53825 1 459 . 1 . 1 299 299 SER H H 1 8.335 . . . . . . . . 320 S HN . 53825 1 460 . 1 . 1 299 299 SER N N 15 115.233 . . . . . . . . 320 S N . 53825 1 461 . 1 . 1 300 300 GLN H H 1 8.411 . . . . . . . . 321 Q HN . 53825 1 462 . 1 . 1 300 300 GLN N N 15 122.284 . . . . . . . . 321 Q N . 53825 1 463 . 1 . 1 301 301 ALA H H 1 8.285 . . . . . . . . 322 A HN . 53825 1 464 . 1 . 1 301 301 ALA N N 15 125.219 . . . . . . . . 322 A N . 53825 1 465 . 1 . 1 302 302 GLU H H 1 8.361 . . . . . . . . 323 E HN . 53825 1 466 . 1 . 1 302 302 GLU N N 15 120.577 . . . . . . . . 323 E N . 53825 1 467 . 1 . 1 303 303 ASP H H 1 8.341 . . . . . . . . 324 D HN . 53825 1 468 . 1 . 1 303 303 ASP N N 15 122.471 . . . . . . . . 324 D N . 53825 1 469 . 1 . 1 304 304 THR H H 1 8.292 . . . . . . . . 325 T HN . 53825 1 470 . 1 . 1 304 304 THR N N 15 115.224 . . . . . . . . 325 T N . 53825 1 471 . 1 . 1 305 305 ARG H H 1 7.973 . . . . . . . . 326 R HN . 53825 1 472 . 1 . 1 305 305 ARG N N 15 123.680 . . . . . . . . 326 R N . 53825 1 473 . 1 . 1 310 310 ARG H H 1 7.450 . . . . . . . . 331 R HN . 53825 1 474 . 1 . 1 310 310 ARG N N 15 118.119 . . . . . . . . 331 R N . 53825 1 475 . 1 . 1 312 312 GLU H H 1 7.010 . . . . . . . . 333 E HN . 53825 1 476 . 1 . 1 312 312 GLU N N 15 120.492 . . . . . . . . 333 E N . 53825 1 477 . 1 . 1 313 313 HIS H H 1 8.845 . . . . . . . . 334 H HN . 53825 1 478 . 1 . 1 313 313 HIS N N 15 117.619 . . . . . . . . 334 H N . 53825 1 479 . 1 . 1 314 314 GLN H H 1 9.425 . . . . . . . . 335 Q HN . 53825 1 480 . 1 . 1 314 314 GLN N N 15 121.889 . . . . . . . . 335 Q N . 53825 1 481 . 1 . 1 315 315 LEU H H 1 9.024 . . . . . . . . 336 L HN . 53825 1 482 . 1 . 1 315 315 LEU N N 15 118.875 . . . . . . . . 336 L N . 53825 1 483 . 1 . 1 316 316 ARG H H 1 7.451 . . . . . . . . 337 R HN . 53825 1 484 . 1 . 1 316 316 ARG N N 15 119.177 . . . . . . . . 337 R N . 53825 1 485 . 1 . 1 317 317 GLN H H 1 7.668 . . . . . . . . 338 Q HN . 53825 1 486 . 1 . 1 317 317 GLN N N 15 118.386 . . . . . . . . 338 Q N . 53825 1 487 . 1 . 1 319 319 ASN H H 1 8.598 . . . . . . . . 340 N HN . 53825 1 488 . 1 . 1 319 319 ASN N N 15 119.348 . . . . . . . . 340 N N . 53825 1 489 . 1 . 1 320 320 ASP H H 1 8.338 . . . . . . . . 341 D HN . 53825 1 490 . 1 . 1 320 320 ASP N N 15 121.760 . . . . . . . . 341 D N . 53825 1 491 . 1 . 1 321 321 MET H H 1 7.376 . . . . . . . . 342 M HN . 53825 1 492 . 1 . 1 321 321 MET N N 15 116.047 . . . . . . . . 342 M N . 53825 1 493 . 1 . 1 322 322 GLY H H 1 7.603 . . . . . . . . 343 G HN . 53825 1 494 . 1 . 1 322 322 GLY N N 15 105.094 . . . . . . . . 343 G N . 53825 1 495 . 1 . 1 323 323 PHE H H 1 7.757 . . . . . . . . 344 F HN . 53825 1 496 . 1 . 1 323 323 PHE N N 15 121.238 . . . . . . . . 344 F N . 53825 1 497 . 1 . 1 324 324 PHE H H 1 8.585 . . . . . . . . 345 F HN . 53825 1 498 . 1 . 1 324 324 PHE N N 15 123.386 . . . . . . . . 345 F N . 53825 1 499 . 1 . 1 325 325 ASP H H 1 8.867 . . . . . . . . 346 D HN . 53825 1 500 . 1 . 1 325 325 ASP N N 15 124.265 . . . . . . . . 346 D N . 53825 1 501 . 1 . 1 326 326 PHE H H 1 8.753 . . . . . . . . 347 F HN . 53825 1 502 . 1 . 1 326 326 PHE N N 15 129.933 . . . . . . . . 347 F N . 53825 1 503 . 1 . 1 327 327 ASP H H 1 8.410 . . . . . . . . 348 D HN . 53825 1 504 . 1 . 1 327 327 ASP N N 15 117.031 . . . . . . . . 348 D N . 53825 1 505 . 1 . 1 328 328 ARG H H 1 8.040 . . . . . . . . 349 R HN . 53825 1 506 . 1 . 1 328 328 ARG N N 15 121.471 . . . . . . . . 349 R N . 53825 1 507 . 1 . 1 329 329 ASN H H 1 8.494 . . . . . . . . 350 N HN . 53825 1 508 . 1 . 1 329 329 ASN N N 15 119.841 . . . . . . . . 350 N N . 53825 1 509 . 1 . 1 330 330 VAL H H 1 8.414 . . . . . . . . 351 V HN . 53825 1 510 . 1 . 1 330 330 VAL N N 15 119.199 . . . . . . . . 351 V N . 53825 1 511 . 1 . 1 331 331 ALA H H 1 7.717 . . . . . . . . 352 A HN . 53825 1 512 . 1 . 1 331 331 ALA N N 15 121.982 . . . . . . . . 352 A N . 53825 1 513 . 1 . 1 332 332 ALA H H 1 8.291 . . . . . . . . 353 A HN . 53825 1 514 . 1 . 1 332 332 ALA N N 15 121.380 . . . . . . . . 353 A N . 53825 1 515 . 1 . 1 333 333 LEU H H 1 8.038 . . . . . . . . 354 L HN . 53825 1 516 . 1 . 1 333 333 LEU N N 15 120.100 . . . . . . . . 354 L N . 53825 1 517 . 1 . 1 334 334 ARG H H 1 8.654 . . . . . . . . 355 R HN . 53825 1 518 . 1 . 1 334 334 ARG N N 15 120.591 . . . . . . . . 355 R N . 53825 1 519 . 1 . 1 335 335 ARG H H 1 7.675 . . . . . . . . 356 R HN . 53825 1 520 . 1 . 1 335 335 ARG N N 15 119.302 . . . . . . . . 356 R N . 53825 1 521 . 1 . 1 336 336 SER H H 1 8.012 . . . . . . . . 357 S HN . 53825 1 522 . 1 . 1 336 336 SER N N 15 112.685 . . . . . . . . 357 S N . 53825 1 523 . 1 . 1 337 337 GLY H H 1 7.682 . . . . . . . . 358 G HN . 53825 1 524 . 1 . 1 337 337 GLY N N 15 112.298 . . . . . . . . 358 G N . 53825 1 525 . 1 . 1 338 338 GLY H H 1 9.040 . . . . . . . . 359 G HN . 53825 1 526 . 1 . 1 338 338 GLY N N 15 105.861 . . . . . . . . 359 G N . 53825 1 527 . 1 . 1 339 339 SER H H 1 7.059 . . . . . . . . 360 S HN . 53825 1 528 . 1 . 1 339 339 SER N N 15 113.661 . . . . . . . . 360 S N . 53825 1 529 . 1 . 1 341 341 GLN H H 1 8.522 . . . . . . . . 362 Q HN . 53825 1 530 . 1 . 1 341 341 GLN N N 15 119.625 . . . . . . . . 362 Q N . 53825 1 531 . 1 . 1 342 342 GLY H H 1 8.054 . . . . . . . . 363 G HN . 53825 1 532 . 1 . 1 342 342 GLY N N 15 104.506 . . . . . . . . 363 G N . 53825 1 533 . 1 . 1 343 343 ALA H H 1 7.917 . . . . . . . . 364 A HN . 53825 1 534 . 1 . 1 343 343 ALA N N 15 124.993 . . . . . . . . 364 A N . 53825 1 535 . 1 . 1 345 345 ASP H H 1 7.710 . . . . . . . . 366 D HN . 53825 1 536 . 1 . 1 345 345 ASP N N 15 117.190 . . . . . . . . 366 D N . 53825 1 537 . 1 . 1 346 346 SER H H 1 7.473 . . . . . . . . 367 S HN . 53825 1 538 . 1 . 1 346 346 SER N N 15 114.322 . . . . . . . . 367 S N . 53825 1 539 . 1 . 1 347 347 LEU H H 1 8.190 . . . . . . . . 368 L HN . 53825 1 540 . 1 . 1 347 347 LEU N N 15 122.678 . . . . . . . . 368 L N . 53825 1 541 . 1 . 1 349 349 ASN H H 1 7.892 . . . . . . . . 370 N HN . 53825 1 542 . 1 . 1 349 349 ASN N N 15 115.891 . . . . . . . . 370 N N . 53825 1 543 . 1 . 1 350 350 GLY H H 1 7.863 . . . . . . . . 371 G HN . 53825 1 544 . 1 . 1 350 350 GLY N N 15 107.634 . . . . . . . . 371 G N . 53825 1 545 . 1 . 1 352 352 VAL H H 1 7.366 . . . . . . . . 373 V HN . 53825 1 546 . 1 . 1 352 352 VAL N N 15 119.174 . . . . . . . . 373 V N . 53825 1 stop_ save_