BMRB Entry 53824

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53824

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Title:
1H-15N Chemical shifts of Dsk2 I45A mutant from S. cerevisiae
Deposition date:
2026-05-28
Original release date:
2026-06-29
Authors:
Acharya, Nirbhik; Castaneda, Carlos
Citation:

Citation: Niblo, Jessica; Acharya, Nirbhik; Watkins, Maxwell; Castaneda, Carlos; Sukenik, Shahar. "Intramolecular interactions between folded and disordered regions shape ubiquilin structure and function "  Adv. Sci. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 373 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pE-SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSLNIHIKSGQDKWEVNVAP ESTVLQFKEAINKANGIPVA NQRLAYSGKILKDDQTVESY HIQDGHSVHLVKSQPKPQTA SAAGANNATATGAAAGTGAT PNMSSGQSAGFNPLADLTSA RYAGYLNMPSADMFGPDGGA LNNDSNNQDELLRMMENPIF QSQMNEMLSNPQMLDFMIQS NPQLQAMGPQARQMLQSPMF RQMLTNPDMIRQSMQFARMM DPNAGMGSAGGAASAFPAPG GDAPEEGSNTNTTSSSNTGN NAGTNAGTNAGANTAANPFA SLLNPALNPFANAGNAASTG MPAFDPALLASMFQPPVQAS QAEDTRPPEERYEHQLRQLN DMGFFDFDRNVAALRRSGGS VQGALDSLLNGDV

Data sets:
Data typeCount
15N chemical shifts250
1H chemical shifts250

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Dsk2 I45A1

Entities:

Entity 1, Dsk2 I45A 373 residues - Formula weight is not available

The isoleucine at position 45 in the original sequence of Dsk2 has been replaced by an alanine.

1   METSERLEUASNILEHISILELYSSERGLY
2   GLNASPLYSTRPGLUVALASNVALALAPRO
3   GLUSERTHRVALLEUGLNPHELYSGLUALA
4   ILEASNLYSALAASNGLYILEPROVALALA
5   ASNGLNARGLEUALATYRSERGLYLYSILE
6   LEULYSASPASPGLNTHRVALGLUSERTYR
7   HISILEGLNASPGLYHISSERVALHISLEU
8   VALLYSSERGLNPROLYSPROGLNTHRALA
9   SERALAALAGLYALAASNASNALATHRALA
10   THRGLYALAALAALAGLYTHRGLYALATHR
11   PROASNMETSERSERGLYGLNSERALAGLY
12   PHEASNPROLEUALAASPLEUTHRSERALA
13   ARGTYRALAGLYTYRLEUASNMETPROSER
14   ALAASPMETPHEGLYPROASPGLYGLYALA
15   LEUASNASNASPSERASNASNGLNASPGLU
16   LEULEUARGMETMETGLUASNPROILEPHE
17   GLNSERGLNMETASNGLUMETLEUSERASN
18   PROGLNMETLEUASPPHEMETILEGLNSER
19   ASNPROGLNLEUGLNALAMETGLYPROGLN
20   ALAARGGLNMETLEUGLNSERPROMETPHE
21   ARGGLNMETLEUTHRASNPROASPMETILE
22   ARGGLNSERMETGLNPHEALAARGMETMET
23   ASPPROASNALAGLYMETGLYSERALAGLY
24   GLYALAALASERALAPHEPROALAPROGLY
25   GLYASPALAPROGLUGLUGLYSERASNTHR
26   ASNTHRTHRSERSERSERASNTHRGLYASN
27   ASNALAGLYTHRASNALAGLYTHRASNALA
28   GLYALAASNTHRALAALAASNPROPHEALA
29   SERLEULEUASNPROALALEUASNPROPHE
30   ALAASNALAGLYASNALAALASERTHRGLY
31   METPROALAPHEASPPROALALEULEUALA
32   SERMETPHEGLNPROPROVALGLNALASER
33   GLNALAGLUASPTHRARGPROPROGLUGLU
34   ARGTYRGLUHISGLNLEUARGGLNLEUASN
35   ASPMETGLYPHEPHEASPPHEASPARGASN
36   VALALAALALEUARGARGSERGLYGLYSER
37   VALGLNGLYALALEUASPSERLEULEUASN
38   GLYASPVAL

Samples:

sample_1: Dsk2 I45A, [U-99% 15N], 50 uM; sodium phosphate 20 mM; sodium azide 0.02%; EDTA 0.5 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

CcpNMR v2.5.1 - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

UNP P48510

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks