BMRB Entry 5369
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5369
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Sharpe, B.; Matthews, J.; Kwan, A.; Newton, A.; Gell, D.; Crossley, M.; Mackay, J.. "A New Zinc Binding Fold Underlines the Versatility of Zinc Binding Modules in
Protein Evolution" Structure 10, 636-648 (2002).
PubMed: 12015147
Assembly members:
CREB Binding Protein, polymer, 27 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
CREB Binding Protein: EVRACSLPHCRTMKNVLNHM
THCQAGK
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 168 |
| coupling constants | 10 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CREB Binding Protein | 1 |
| 2 | Zinc ion | 2 |
Entities:
Entity 1, CREB Binding Protein 27 residues - Formula weight is not available
| 1 | GLU | VAL | ARG | ALA | CYS | SER | LEU | PRO | HIS | CYS | ||||
| 2 | ARG | THR | MET | LYS | ASN | VAL | LEU | ASN | HIS | MET | ||||
| 3 | THR | HIS | CYS | GLN | ALA | GLY | LYS |
Entity 2, Zinc ion - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: CREB Binding Protein 0.4 mM; ZINC (II) ION 0.6 mM; TCEP 0.6 mM
sample_cond_1: pH: 6.5; temperature: 275 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | sample_1 | not available | sample_cond_1 |
| 2D TOCSY | sample_1 | not available | sample_cond_1 |
| 2D NOESY | sample_1 | not available | sample_cond_1 |
| E-COSY | sample_1 | not available | sample_cond_1 |
Software:
XWINNMR v2.5 - processing
XEASY v1.3.13 - data analysis
DYANA v1.5 - data analysis
CNS v1.0 - refinement
NMR spectrometers:
- Bruker DRX 600 MHz