BMRB Entry 5344

Title:
1H, 15N Assignment of Neocarzinostatin Apo-Protein Bound to a Synthetic Chromophore   PubMed: 12269815
Deposition date:
2002-04-12
Original release date:
2006-04-05
Authors:
Urbaniak, Michael; Muskett, Frederick; Finucane, Michael; Caddick, Stephen; Woolfson, Derek
Citation:

Citation: Urbaniak, Michael; Muskett, Frederick; Finucane, M.; Caddick, Stephen; Woolfson, Derek. "Solution Structure of a Novel Chromoprotein dervived from apo-Neocarzinostatin and a Synthetic Chromophore "  Biochemistry 41, 11731-11739 (2002).

Assembly members:

Assembly members:
Neocarzinostatin apo-protein, polymer, 122 residues, 12213 Da.
SM, non-polymer, 150.360 Da.

Natural source:

Natural source:   Common Name: Streptomyces carzinostaticus   Taxonomy ID: 1897   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Streptomyces carzinostaticus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pCANTABB

Experimental source:

Natural source:   Common Name: Streptomyces carzinostaticus   Taxonomy ID: 1897   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Streptomyces carzinostaticus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pCANTABB

Data sets:
Data typeCount
15N chemical shifts113
1H chemical shifts552

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Neocarzinostatin apo-protein1
2Napthoate ester2

Entities:

Entity 1, Neocarzinostatin apo-protein 122 residues - 12213 Da.

1   HISHISHISHISHISHISLEUGLNGLYALA
2   ALAPROTHRALATHRVALTHRPROSERSER
3   GLYLEUSERASPGLYTHRVALVALLYSVAL
4   ALAGLYALAGLYLEUGLNALAGLYTHRALA
5   TYRASPVALGLYGLNCYSALATRPVALASP
6   THRGLYVALLEUALACYSASNPROALAASP
7   PHESERSERVALTHRALAASPALAASNGLY
8   SERALASERTHRSERLEUTHRVALARGARG
9   SERPHEGLUGLYPHELEUPHEASPGLYTHR
10   ARGTRPGLYTHRVALASPCYSTHRTHRALA
11   ALACYSGLNVALGLYLEUSERASPALAALA
12   GLYASNGLYPROGLUGLYVALALAILESER
13   PHEASN

Entity 2, Napthoate ester - Sm - 150.360 Da.

1   SM

Samples:

sample_1: Neocarzinostatin apo-protein, [U-15N], 1.0 mM; 2-hydroxy-7-methoxy-5-methyl-naphthalene-1-carboxylic acid meso-2 5-dihydroxy-cyclopent-3-enyl ester 1.0 mM; D2O 10 % v/v; phosphate buffer 25 mM; TSP 0.1 mM; sodium azide 0.005 % w/w

condition_1: pH: 5.0; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-1H-15N TOCSYsample_1not availablecondition_1
3D 1H-1H-15N NOESYsample_1not availablecondition_1
3D HNHAsample_1not availablecondition_1
3D HNHBsample_1not availablecondition_1
2D 1H-15N HSQCsample_1not availablecondition_1
2D 1H-1H NOESYsample_1not availablecondition_1
HNHBsample_1not availablecondition_1
NOESYsample_1not availablecondition_1

Software:

NMRpipe - processing

XEASY - on-screen assignments

DYANA - Structure calculation

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian UnityPlus 500 MHz

Related Database Links:

BMRB 1766 5343 5969 6888 695
PDB
DBJ BAA01764 BAB83894
GB AAB28103 AAM77994
SP P0A3R9 P0A3S0
AlphaFold P0A3S0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks