BMRB Entry 5343

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5343

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Title:
1H, 13C, 15N Assignment of Neocarzinostatin Apo-Protein
Deposition date:
2002-04-12
Original release date:
2006-04-05
Authors:
Muskett, Frederick; Stoneman, Rhys; Caddick, Stephen; Woolfson, Derek
Citation:

Citation: Urbaniak, Michael; Muskett, Frederick; Finucane, M.; Caddick, Stephen; Woolfson, Derek. "Solution structure of a novel chromoprotein derived from apo-neocarzinostatin and a synthetic chromophore."  Biochemistry 41, 11731-11739 (2002).
PubMed: 12269815

Assembly members:

Assembly members:
Neocarzinostatin, polymer, 122 residues, 12213 Da.

Natural source:

Natural source:   Common Name: Streptomyces carzinostaticus   Taxonomy ID: 1897   Superkingdom: Bacteria   Kingdom: Not applicable   Genus/species: Streptomyces carzinostaticus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Neocarzinostatin: HHHHHHLQGAAPTATVTPSS GLSDGTVVKVAGAGLQAGTA YDVGQCAWVDTGVLACNPAD FSSVTADANGSASTSLTVRR SFEGFLFDGTRWGTVDCTTA ACQVGLSDAAGNGPEGVAIS FN

Data sets:
Data typeCount
1H chemical shifts643
13C chemical shifts386
15N chemical shifts118

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Neocarzinostatin apo-protein1

Entities:

Entity 1, Neocarzinostatin apo-protein 122 residues - 12213 Da.

1   HISHISHISHISHISHISLEUGLNGLYALA
2   ALAPROTHRALATHRVALTHRPROSERSER
3   GLYLEUSERASPGLYTHRVALVALLYSVAL
4   ALAGLYALAGLYLEUGLNALAGLYTHRALA
5   TYRASPVALGLYGLNCYSALATRPVALASP
6   THRGLYVALLEUALACYSASNPROALAASP
7   PHESERSERVALTHRALAASPALAASNGLY
8   SERALASERTHRSERLEUTHRVALARGARG
9   SERPHEGLUGLYPHELEUPHEASPGLYTHR
10   ARGTRPGLYTHRVALASPCYSTHRTHRALA
11   ALACYSGLNVALGLYLEUSERASPALAALA
12   GLYASNGLYPROGLUGLYVALALAILESER
13   PHEASN

Samples:

sample_1: Neocarzinostatin, [U-13C; U-15N], 1 mM; phosphate buffer 25 mM; sodium azide 0.005 % w/w

conditions_1: pH: 5.0; temperature: 308 K; ionic strength: 50 mM

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availableconditions_1
1H1H15N TOCSYHSQCsample_1not availableconditions_1
1H1H15N NOESYHSQCsample_1not availableconditions_1
1H13C15N CBCANHsample_1not availableconditions_1
1H13C15N CBCA(CO)NHsample_1not availableconditions_1
1H13C13C HCCHTOCSYsample_1not availableconditions_1
1H13C13C HNCOsample_1not availableconditions_1
1H1H TOCSYsample_1not availableconditions_1
1H1H NOESYsample_1not availableconditions_1
1H1H15N HNHAsample_1not availableconditions_1
1H1H15N HNHBsample_1not availableconditions_1

Software:

NMRpipe - processing

CYANA - refinement

Sparky - assignment

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian UnityPlus 500 MHz

Related Database Links:

BMRB 1766 5344 5969 6888 695
PDB
DBJ BAA01764 BAB83894
GB AAB28103 AAM77994
SP P0A3R9 P0A3S0
AlphaFold P0A3R9 P0A3S0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks