BMRB Entry 53223

Name: Nucleobase and ribose protons of asymmeric bulge of human U6 snRNA
Published: 2025-11-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53223

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Nucleobase and ribose protons of asymmeric bulge of human U6 snRNA

Deposition date:

2025-06-11

Original release date:

2025-11-11

Authors:

Kim, Iktae; Bang, Kyeong-Mi; An, So Young; Park, Changkon; Shin, Ji-Yeon; Kim, Youngim; Song, Hyun Kyu; Suh, Jeong-Yong; Kim, Nak-Kyoon

Citation:

Citation: Kim, Iktae; Bang, Kyeong-Mi; An, So Young; Park, Changkon; Shin, Ji-Yeon; Kim, Youngim; Song, Hyun Kyu; Suh, Jeong-Yong; Kim, Nak-Kyoon. "Structural investigation of human U6 snRNA recognition by spliceosomal recycling factor SART3 RNA recognition motifs" FEBS J. ., .-. (2025).
PubMed: 41046346

Assembly members:

Assembly members:
entity_1, polymer, 22 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGAACGAUACAGAGAAGAUU AG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	22
1H chemical shifts	51

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Asymmetric bulge of human U6 snRNA	1

Entities:

Entity 1, Asymmetric bulge of human U6 snRNA 22 residues - Formula weight is not available

1

G

A

C

G

A

U

A

C

2

A

G

A

G

A

G

A

U

3

A

G

Samples:

sample_1: Asymmetric bulge of human U6 snRNA 900 uM; Sodium phosphate 20 mM; Sodium chloride 100 mM

sample_2: Asymmetric bulge of human U6 snRNA, [U-100% 13C; U-100% 15N], 100 uM; Sodium phosphate 20 mM; Sodium chloride 100 mM

sample_conditions_1: ionic strength: 120 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment, data analysis, peak picking

TOPSPIN - processing

NMR spectrometers:

Bruker AVANCE III 800 MHz