BMRB Entry 5299
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PDB ID: 1klq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5299
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Luo, X.; Tang, Z.; Rizo, J.; Yu, H.. "The Mad2 Spindle Checkpoint Protein Undergoes Similar Major Conformational
Changes upon Binding to Either Mad1 or Cdc20 " Mol. Cell 9, 59-71 (2002).
PubMed: 11804586
Assembly members:
mitotic checkpoint protein Mad2, polymer, 197 residues, Formula weight is not available
Mad2-binding peptide, polymer, 12 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
mitotic checkpoint protein Mad2: GSITLRGSAEIVAEFFSFGI
NSILYQRGIYPSETFTRVQK
YGLTLLVTTDLELIKYLNNV
VEQLKDWLYKCSVQKLVVVI
SNIESGEVLERWQFDIECDK
TAKDDSAPREKSQKAIQDEI
RSVIRQITATVTFLPLLEVS
CSFDLLIYTDKDLVVPEKWE
ESGPQFITNSEEVRLRSFTT
TIHKVNSMVAYKIPVND
Mad2-binding peptide: SWYSYPPPQRAV
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 1183 |
| 13C chemical shifts | 749 |
| 15N chemical shifts | 213 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A | 1 |
| 2 | Mad2-binding peptide | 2 |
Entities:
Entity 1, MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A 197 residues - Formula weight is not available
| 1 | GLY | SER | ILE | THR | LEU | ARG | GLY | SER | ALA | GLU | ||||
| 2 | ILE | VAL | ALA | GLU | PHE | PHE | SER | PHE | GLY | ILE | ||||
| 3 | ASN | SER | ILE | LEU | TYR | GLN | ARG | GLY | ILE | TYR | ||||
| 4 | PRO | SER | GLU | THR | PHE | THR | ARG | VAL | GLN | LYS | ||||
| 5 | TYR | GLY | LEU | THR | LEU | LEU | VAL | THR | THR | ASP | ||||
| 6 | LEU | GLU | LEU | ILE | LYS | TYR | LEU | ASN | ASN | VAL | ||||
| 7 | VAL | GLU | GLN | LEU | LYS | ASP | TRP | LEU | TYR | LYS | ||||
| 8 | CYS | SER | VAL | GLN | LYS | LEU | VAL | VAL | VAL | ILE | ||||
| 9 | SER | ASN | ILE | GLU | SER | GLY | GLU | VAL | LEU | GLU | ||||
| 10 | ARG | TRP | GLN | PHE | ASP | ILE | GLU | CYS | ASP | LYS | ||||
| 11 | THR | ALA | LYS | ASP | ASP | SER | ALA | PRO | ARG | GLU | ||||
| 12 | LYS | SER | GLN | LYS | ALA | ILE | GLN | ASP | GLU | ILE | ||||
| 13 | ARG | SER | VAL | ILE | ARG | GLN | ILE | THR | ALA | THR | ||||
| 14 | VAL | THR | PHE | LEU | PRO | LEU | LEU | GLU | VAL | SER | ||||
| 15 | CYS | SER | PHE | ASP | LEU | LEU | ILE | TYR | THR | ASP | ||||
| 16 | LYS | ASP | LEU | VAL | VAL | PRO | GLU | LYS | TRP | GLU | ||||
| 17 | GLU | SER | GLY | PRO | GLN | PHE | ILE | THR | ASN | SER | ||||
| 18 | GLU | GLU | VAL | ARG | LEU | ARG | SER | PHE | THR | THR | ||||
| 19 | THR | ILE | HIS | LYS | VAL | ASN | SER | MET | VAL | ALA | ||||
| 20 | TYR | LYS | ILE | PRO | VAL | ASN | ASP |
Entity 2, Mad2-binding peptide 12 residues - Formula weight is not available
| 1 | SER | TRP | TYR | SER | TYR | PRO | PRO | PRO | GLN | ARG | ||||
| 2 | ALA | VAL |
Related Database Links:
| REF | XP_001084989 NP_002349 |
| SWISS-PROT | Q13257 |
| GenBank | AAH70283 AAH00356 AAH05945 AAC50781 AAC52060 |
| DBJ | BAG36562 BAD97153 BAE01009 BAB63410 |
| EMBL | CAA03943 |
| PDB | 2V64 1S2H 2QYF 1GO4 1KLQ 1DUJ |
| BMRB | 4775 5299 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks