BMRB Entry 5270

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5270

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Title:
Backbone 1H, 15N and 13C resonance assignments of YqgF, an Escherichia coli protein of unknown structure and function
Deposition date:
2002-01-31
Original release date:
2003-04-09
Authors:
Liu, Dingjiang; Repaka, Prasanti; Taremi, Shane; Wyss, Daniel
Citation:

Citation: Liu, Dingjiang; Repaka, Prasanti; Taremi, Shane; Wyss, Daniel. "Letter to the Editor: Backbone 1H, 15N and 13C Resonance Assignments of YqgF, an Escherichia coli Protein of unknown Structure and Function "  J. Biomol. NMR 23, 159-160 (2002).
PubMed: 12153044

Assembly members:

Assembly members:
YqgF, polymer, 147 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
YqgF: RGSMADIGSMSGTLLAFDFG TKSIGVAVGQRITGTARPLP AIKAQDGTPDWNIIERLLKE WQPDEIIVGLPLNMDGTEQP LTARARKFANRIHGRFGVEV KLHDERLSTVEARSGLFEQG GYRALNKGKVDSASAVIILE SYFEQGY

Data sets:
Data typeCount
1H chemical shifts213
13C chemical shifts340
15N chemical shifts111

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YqgF1

Entities:

Entity 1, YqgF 147 residues - Formula weight is not available

1   ARGGLYSERMETALAASPILEGLYSERMET
2   SERGLYTHRLEULEUALAPHEASPPHEGLY
3   THRLYSSERILEGLYVALALAVALGLYGLN
4   ARGILETHRGLYTHRALAARGPROLEUPRO
5   ALAILELYSALAGLNASPGLYTHRPROASP
6   TRPASNILEILEGLUARGLEULEULYSGLU
7   TRPGLNPROASPGLUILEILEVALGLYLEU
8   PROLEUASNMETASPGLYTHRGLUGLNPRO
9   LEUTHRALAARGALAARGLYSPHEALAASN
10   ARGILEHISGLYARGPHEGLYVALGLUVAL
11   LYSLEUHISASPGLUARGLEUSERTHRVAL
12   GLUALAARGSERGLYLEUPHEGLUGLNGLY
13   GLYTYRARGALALEUASNLYSGLYLYSVAL
14   ASPSERALASERALAVALILEILELEUGLU
15   SERTYRPHEGLUGLNGLYTYR

Samples:

sample_1: YqgF, [U-15N], 0.3 – 1.0 mM; KiPO4 75 mM

sample_2: YqgF, [U-13C; U-15N], 0.5 – 1.0 mM; KiPO4 75 mM

Ex-cond_1: pH: 7.5; temperature: 298 K; ionic strength: 0.45 M

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HN(CO)not availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCACONNHnot availablenot availablenot available

Software:

Felix v2000 - process

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 5758
PDB
DBJ BAB37248 BAE77012 BAG78741 BAI27233 BAI32263
EMBL CAP77386 CAQ33259 CAQ90381 CAQ99897 CAR04466
GB AAA69116 AAC75986 AAG58080 AAN44421 AAN81983
REF NP_289521 NP_311852 NP_417424 NP_708714 NP_755410
SP A1AFD5 A7ZR72 A8A489 B1IT58 B1LDF8
AlphaFold A1AFD5 A7ZR72 A8A489 B1IT58 B1LDF8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks