BMRB Entry 52260

Name: 15N relaxation rates for apo WT PHPT1 (R1, R2, NOE)
Published: 2024-05-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52260

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

15N relaxation rates for apo WT PHPT1 (R1, R2, NOE)

Deposition date:

2024-01-05

Original release date:

2024-05-15

Authors:

Zavala, Erik; Dansereau, Stephen; Loria, Joseph

Citation:

Citation: Zavala, Erik; Dansereau, Stephen; Burke, Michael; Lipchock, James; Maschietto, Federica; Batista, Victor; Loria, Joseph. "A salt bridge of the C-terminal carboxyl group regulates PHPT1 substrate affinity and catalytic activity" Protein Sci. 33, e5009-e5009 (2024).
PubMed: 38747379

Assembly members:

Assembly members:
entity_1, polymer, 125 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-43.1b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAVADLALIPDVDIDSDGVF KYVLIRVHSAPRSGAPAAES KEIVRGYKWAEYHADIYDKV SGDMQKQGCDCECLGGGRIS HQSQDKKIHVYGYSMAYGPA QHAISTEKIKAKYPDYEVTW ANDGY

Data sets:

heteronucl_NOEs
- heteronucl_NOEs_1
heteronucl_T1_relaxation
- heteronucl_T1_relaxation_1
heteronucl_T2_relaxation
- heteronucl_T2_relaxation_1

Data type	Count
T1 relaxation values	87
T2 relaxation values	86
heteronuclear NOE values	85

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PHPT1	1

Entities:

Entity 1, PHPT1 125 residues - Formula weight is not available

1

MET

ALA

VAL

ALA

ASP

LEU

ALA

LEU

ILE

PRO

2

ASP

VAL

ASP

ILE

ASP

SER

ASP

GLY

VAL

PHE

3

LYS

TYR

VAL

LEU

ILE

ARG

VAL

HIS

SER

ALA

4

PRO

ARG

SER

GLY

ALA

PRO

ALA

GLU

SER

5

LYS

GLU

ILE

VAL

ARG

GLY

TYR

LYS

TRP

ALA

6

GLU

TYR

HIS

ALA

ASP

ILE

TYR

ASP

LYS

VAL

7

SER

GLY

ASP

MET

GLN

LYS

GLN

GLY

CYS

ASP

8

CYS

GLU

CYS

LEU

GLY

ARG

ILE

SER

9

HIS

GLN

SER

GLN

ASP

LYS

ILE

HIS

VAL

10

TYR

GLY

TYR

SER

MET

ALA

TYR

GLY

PRO

ALA

11

GLN

HIS

ALA

ILE

SER

THR

GLU

LYS

ILE

LYS

12

ALA

LYS

TYR

PRO

ASP

TYR

GLU

VAL

THR

TRP

13

ALA

ASN

ASP

GLY

TYR

Samples:

sample_1: Apo WT PHPT1, [U-100% 15N], 235 uM; Tris HCL 50 mM; NaCl 50 mM; DTT 30 mM; sodium azide 0.02%

sample_conditions_1: pH: 7.2; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
T1/R1 relaxation	sample_1	isotropic	sample_conditions_1
T2/R2 relaxation	sample_1	isotropic	sample_conditions_1
1H-15N heteronoe	sample_1	isotropic	sample_conditions_1

Software:

VNMRj - collection

NMRPipe - processing

NMRFAM-SPARKY - data analysis

House written software - data analysis

NMR spectrometers:

Varian INOVA 700 MHz