data_52260 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 52260 _Entry.Title ; 15N relaxation rates for apo WT PHPT1 (R1, R2, NOE) ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2024-01-05 _Entry.Accession_date 2024-01-05 _Entry.Last_release_date 2024-01-06 _Entry.Original_release_date 2024-01-06 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID . _Entry.Generated_software_label . _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Erik Zavala . . . 0000-0001-6293-3461 52260 2 Stephen Dansereau . . . . 52260 3 Joseph Loria . P. . . 52260 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID heteronucl_NOEs 1 52260 heteronucl_T1_relaxation 1 52260 heteronucl_T2_relaxation 1 52260 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID 'T1 relaxation values' 87 52260 'T2 relaxation values' 86 52260 'heteronuclear NOE values' 85 52260 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2024-05-15 . original BMRB . 52260 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 52261 '15N relaxation rates for apo G126 PHPT1' 52260 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 52260 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID 38747379 _Citation.DOI . _Citation.Full_citation . _Citation.Title ; A salt bridge of the C-terminal carboxyl group regulates PHPT1 substrate affinity and catalytic activity ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Protein Sci.' _Citation.Journal_name_full . _Citation.Journal_volume 33 _Citation.Journal_issue 6 _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first e5009 _Citation.Page_last e5009 _Citation.Year 2024 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Erik Zavala . . . . 52260 1 2 Stephen Dansereau . . . . 52260 1 3 Michael Burke . J. . . 52260 1 4 James Lipchock . M. . . 52260 1 5 Federica Maschietto . . . . 52260 1 6 Victor Batista . . . . 52260 1 7 Joseph Loria . P. . . 52260 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 52260 _Assembly.ID 1 _Assembly.Name 'Apo WT PHPT1' _Assembly.BMRB_code . _Assembly.Number_of_components 1 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states yes _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 PHPT1 1 $entity_1 . . yes native yes no . . . 52260 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 52260 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MAVADLALIPDVDIDSDGVF KYVLIRVHSAPRSGAPAAES KEIVRGYKWAEYHADIYDKV SGDMQKQGCDCECLGGGRIS HQSQDKKIHVYGYSMAYGPA QHAISTEKIKAKYPDYEVTW ANDGY ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states yes _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 125 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not available' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . MET . 52260 1 2 . ALA . 52260 1 3 . VAL . 52260 1 4 . ALA . 52260 1 5 . ASP . 52260 1 6 . LEU . 52260 1 7 . ALA . 52260 1 8 . LEU . 52260 1 9 . ILE . 52260 1 10 . PRO . 52260 1 11 . ASP . 52260 1 12 . VAL . 52260 1 13 . ASP . 52260 1 14 . ILE . 52260 1 15 . ASP . 52260 1 16 . SER . 52260 1 17 . ASP . 52260 1 18 . GLY . 52260 1 19 . VAL . 52260 1 20 . PHE . 52260 1 21 . LYS . 52260 1 22 . TYR . 52260 1 23 . VAL . 52260 1 24 . LEU . 52260 1 25 . ILE . 52260 1 26 . ARG . 52260 1 27 . VAL . 52260 1 28 . HIS . 52260 1 29 . SER . 52260 1 30 . ALA . 52260 1 31 . PRO . 52260 1 32 . ARG . 52260 1 33 . SER . 52260 1 34 . GLY . 52260 1 35 . ALA . 52260 1 36 . PRO . 52260 1 37 . ALA . 52260 1 38 . ALA . 52260 1 39 . GLU . 52260 1 40 . SER . 52260 1 41 . LYS . 52260 1 42 . GLU . 52260 1 43 . ILE . 52260 1 44 . VAL . 52260 1 45 . ARG . 52260 1 46 . GLY . 52260 1 47 . TYR . 52260 1 48 . LYS . 52260 1 49 . TRP . 52260 1 50 . ALA . 52260 1 51 . GLU . 52260 1 52 . TYR . 52260 1 53 . HIS . 52260 1 54 . ALA . 52260 1 55 . ASP . 52260 1 56 . ILE . 52260 1 57 . TYR . 52260 1 58 . ASP . 52260 1 59 . LYS . 52260 1 60 . VAL . 52260 1 61 . SER . 52260 1 62 . GLY . 52260 1 63 . ASP . 52260 1 64 . MET . 52260 1 65 . GLN . 52260 1 66 . LYS . 52260 1 67 . GLN . 52260 1 68 . GLY . 52260 1 69 . CYS . 52260 1 70 . ASP . 52260 1 71 . CYS . 52260 1 72 . GLU . 52260 1 73 . CYS . 52260 1 74 . LEU . 52260 1 75 . GLY . 52260 1 76 . GLY . 52260 1 77 . GLY . 52260 1 78 . ARG . 52260 1 79 . ILE . 52260 1 80 . SER . 52260 1 81 . HIS . 52260 1 82 . GLN . 52260 1 83 . SER . 52260 1 84 . GLN . 52260 1 85 . ASP . 52260 1 86 . LYS . 52260 1 87 . LYS . 52260 1 88 . ILE . 52260 1 89 . HIS . 52260 1 90 . VAL . 52260 1 91 . TYR . 52260 1 92 . GLY . 52260 1 93 . TYR . 52260 1 94 . SER . 52260 1 95 . MET . 52260 1 96 . ALA . 52260 1 97 . TYR . 52260 1 98 . GLY . 52260 1 99 . PRO . 52260 1 100 . ALA . 52260 1 101 . GLN . 52260 1 102 . HIS . 52260 1 103 . ALA . 52260 1 104 . ILE . 52260 1 105 . SER . 52260 1 106 . THR . 52260 1 107 . GLU . 52260 1 108 . LYS . 52260 1 109 . ILE . 52260 1 110 . LYS . 52260 1 111 . ALA . 52260 1 112 . LYS . 52260 1 113 . TYR . 52260 1 114 . PRO . 52260 1 115 . ASP . 52260 1 116 . TYR . 52260 1 117 . GLU . 52260 1 118 . VAL . 52260 1 119 . THR . 52260 1 120 . TRP . 52260 1 121 . ALA . 52260 1 122 . ASN . 52260 1 123 . ASP . 52260 1 124 . GLY . 52260 1 125 . TYR . 52260 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 52260 1 . ALA 2 2 52260 1 . VAL 3 3 52260 1 . ALA 4 4 52260 1 . ASP 5 5 52260 1 . LEU 6 6 52260 1 . ALA 7 7 52260 1 . LEU 8 8 52260 1 . ILE 9 9 52260 1 . PRO 10 10 52260 1 . ASP 11 11 52260 1 . VAL 12 12 52260 1 . ASP 13 13 52260 1 . ILE 14 14 52260 1 . ASP 15 15 52260 1 . SER 16 16 52260 1 . ASP 17 17 52260 1 . GLY 18 18 52260 1 . VAL 19 19 52260 1 . PHE 20 20 52260 1 . LYS 21 21 52260 1 . TYR 22 22 52260 1 . VAL 23 23 52260 1 . LEU 24 24 52260 1 . ILE 25 25 52260 1 . ARG 26 26 52260 1 . VAL 27 27 52260 1 . HIS 28 28 52260 1 . SER 29 29 52260 1 . ALA 30 30 52260 1 . PRO 31 31 52260 1 . ARG 32 32 52260 1 . SER 33 33 52260 1 . GLY 34 34 52260 1 . ALA 35 35 52260 1 . PRO 36 36 52260 1 . ALA 37 37 52260 1 . ALA 38 38 52260 1 . GLU 39 39 52260 1 . SER 40 40 52260 1 . LYS 41 41 52260 1 . GLU 42 42 52260 1 . ILE 43 43 52260 1 . VAL 44 44 52260 1 . ARG 45 45 52260 1 . GLY 46 46 52260 1 . TYR 47 47 52260 1 . LYS 48 48 52260 1 . TRP 49 49 52260 1 . ALA 50 50 52260 1 . GLU 51 51 52260 1 . TYR 52 52 52260 1 . HIS 53 53 52260 1 . ALA 54 54 52260 1 . ASP 55 55 52260 1 . ILE 56 56 52260 1 . TYR 57 57 52260 1 . ASP 58 58 52260 1 . LYS 59 59 52260 1 . VAL 60 60 52260 1 . SER 61 61 52260 1 . GLY 62 62 52260 1 . ASP 63 63 52260 1 . MET 64 64 52260 1 . GLN 65 65 52260 1 . LYS 66 66 52260 1 . GLN 67 67 52260 1 . GLY 68 68 52260 1 . CYS 69 69 52260 1 . ASP 70 70 52260 1 . CYS 71 71 52260 1 . GLU 72 72 52260 1 . CYS 73 73 52260 1 . LEU 74 74 52260 1 . GLY 75 75 52260 1 . GLY 76 76 52260 1 . GLY 77 77 52260 1 . ARG 78 78 52260 1 . ILE 79 79 52260 1 . SER 80 80 52260 1 . HIS 81 81 52260 1 . GLN 82 82 52260 1 . SER 83 83 52260 1 . GLN 84 84 52260 1 . ASP 85 85 52260 1 . LYS 86 86 52260 1 . LYS 87 87 52260 1 . ILE 88 88 52260 1 . HIS 89 89 52260 1 . VAL 90 90 52260 1 . TYR 91 91 52260 1 . GLY 92 92 52260 1 . TYR 93 93 52260 1 . SER 94 94 52260 1 . MET 95 95 52260 1 . ALA 96 96 52260 1 . TYR 97 97 52260 1 . GLY 98 98 52260 1 . PRO 99 99 52260 1 . ALA 100 100 52260 1 . GLN 101 101 52260 1 . HIS 102 102 52260 1 . ALA 103 103 52260 1 . ILE 104 104 52260 1 . SER 105 105 52260 1 . THR 106 106 52260 1 . GLU 107 107 52260 1 . LYS 108 108 52260 1 . ILE 109 109 52260 1 . LYS 110 110 52260 1 . ALA 111 111 52260 1 . LYS 112 112 52260 1 . TYR 113 113 52260 1 . PRO 114 114 52260 1 . ASP 115 115 52260 1 . TYR 116 116 52260 1 . GLU 117 117 52260 1 . VAL 118 118 52260 1 . THR 119 119 52260 1 . TRP 120 120 52260 1 . ALA 121 121 52260 1 . ASN 122 122 52260 1 . ASP 123 123 52260 1 . GLY 124 124 52260 1 . TYR 125 125 52260 1 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 52260 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 52260 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 52260 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . pET-43.1b(+) . . . 52260 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 52260 _Sample.ID 1 _Sample.Name 'Apo WT PHPT1' _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '93% H2O/7% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'Apo WT PHPT1' '[U-100% 15N]' . . 1 $entity_1 . . 235 . . uM . . . . 52260 1 2 'Tris HCL' 'natural abundance' . . . . . . 50 . . mM . . . . 52260 1 3 NaCl 'natural abundance' . . . . . . 50 . . mM . . . . 52260 1 4 DTT 'natural abundance' . . . . . . 30 . . mM . . . . 52260 1 5 'sodium azide' 'natural abundance' . . . . . . 0.02 . . % . . . . 52260 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 52260 _Sample_condition_list.ID 1 _Sample_condition_list.Name Simple _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID pH 7.2 . pH 52260 1 temperature 298.15 . K 52260 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 52260 _Software.ID 1 _Software.Type . _Software.Name VNMRj _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID collection . 52260 1 stop_ save_ save_software_2 _Software.Sf_category software _Software.Sf_framecode software_2 _Software.Entry_ID 52260 _Software.ID 2 _Software.Type . _Software.Name NMRPipe _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID processing . 52260 2 stop_ save_ save_software_3 _Software.Sf_category software _Software.Sf_framecode software_3 _Software.Entry_ID 52260 _Software.ID 3 _Software.Type . _Software.Name NMRFAM-SPARKY _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'data analysis' . 52260 3 stop_ save_ save_software_4 _Software.Sf_category software _Software.Sf_framecode software_4 _Software.Entry_ID 52260 _Software.ID 4 _Software.Type . _Software.Name 'House written software' _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'data analysis' . 52260 4 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 52260 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name 'Varian 700' _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Varian _NMR_spectrometer.Model INOVA _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 700 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 52260 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 'T1/R1 relaxation' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 52260 1 2 'T2/R2 relaxation' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 52260 1 3 '1H-15N heteronoe' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 52260 1 stop_ save_ ############################## # Heteronuclear NOE values # ############################## save_heteronucl_NOEs_1 _Heteronucl_NOE_list.Sf_category heteronucl_NOEs _Heteronucl_NOE_list.Sf_framecode heteronucl_NOEs_1 _Heteronucl_NOE_list.Entry_ID 52260 _Heteronucl_NOE_list.ID 1 _Heteronucl_NOE_list.Name 'WT PHPT1 NOE' _Heteronucl_NOE_list.Sample_condition_list_ID 1 _Heteronucl_NOE_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_NOE_list.Spectrometer_frequency_1H 700 _Heteronucl_NOE_list.Heteronuclear_NOE_val_type 'peak height' _Heteronucl_NOE_list.NOE_ref_val 0 _Heteronucl_NOE_list.NOE_ref_description . _Heteronucl_NOE_list.Details . _Heteronucl_NOE_list.Text_data_format . _Heteronucl_NOE_list.Text_data . loop_ _Heteronucl_NOE_experiment.Experiment_ID _Heteronucl_NOE_experiment.Experiment_name _Heteronucl_NOE_experiment.Sample_ID _Heteronucl_NOE_experiment.Sample_label _Heteronucl_NOE_experiment.Sample_state _Heteronucl_NOE_experiment.Entry_ID _Heteronucl_NOE_experiment.Heteronucl_NOE_list_ID 3 '1H-15N heteronoe' . . . 52260 1 stop_ loop_ _Heteronucl_NOE_software.Software_ID _Heteronucl_NOE_software.Software_label _Heteronucl_NOE_software.Method_ID _Heteronucl_NOE_software.Method_label _Heteronucl_NOE_software.Entry_ID _Heteronucl_NOE_software.Heteronucl_NOE_list_ID 4 $software_4 . . 52260 1 stop_ loop_ _Heteronucl_NOE.ID _Heteronucl_NOE.Assembly_atom_ID_1 _Heteronucl_NOE.Entity_assembly_ID_1 _Heteronucl_NOE.Entity_ID_1 _Heteronucl_NOE.Comp_index_ID_1 _Heteronucl_NOE.Seq_ID_1 _Heteronucl_NOE.Comp_ID_1 _Heteronucl_NOE.Atom_ID_1 _Heteronucl_NOE.Atom_type_1 _Heteronucl_NOE.Atom_isotope_number_1 _Heteronucl_NOE.Assembly_atom_ID_2 _Heteronucl_NOE.Entity_assembly_ID_2 _Heteronucl_NOE.Entity_ID_2 _Heteronucl_NOE.Comp_index_ID_2 _Heteronucl_NOE.Seq_ID_2 _Heteronucl_NOE.Comp_ID_2 _Heteronucl_NOE.Atom_ID_2 _Heteronucl_NOE.Atom_type_2 _Heteronucl_NOE.Atom_isotope_number_2 _Heteronucl_NOE.Val _Heteronucl_NOE.Val_err _Heteronucl_NOE.Resonance_ID_1 _Heteronucl_NOE.Resonance_ID_2 _Heteronucl_NOE.Auth_entity_assembly_ID_1 _Heteronucl_NOE.Auth_seq_ID_1 _Heteronucl_NOE.Auth_comp_ID_1 _Heteronucl_NOE.Auth_atom_ID_1 _Heteronucl_NOE.Auth_entity_assembly_ID_2 _Heteronucl_NOE.Auth_seq_ID_2 _Heteronucl_NOE.Auth_comp_ID_2 _Heteronucl_NOE.Auth_atom_ID_2 _Heteronucl_NOE.Entry_ID _Heteronucl_NOE.Heteronucl_NOE_list_ID 1 . 1 1 2 2 ALA N N 15 . 1 1 2 2 ALA H H 1 0.357404 0.0241498 . . . . . . . . . . 52260 1 2 . 1 1 3 3 VAL N N 15 . 1 1 3 3 VAL H H 1 0.460748 0.017032 . . . . . . . . . . 52260 1 3 . 1 1 4 4 ALA N N 15 . 1 1 4 4 ALA H H 1 0.469011 0.0242273 . . . . . . . . . . 52260 1 4 . 1 1 5 5 ASP N N 15 . 1 1 5 5 ASP H H 1 0.508343 0.0235802 . . . . . . . . . . 52260 1 5 . 1 1 6 6 LEU N N 15 . 1 1 6 6 LEU H H 1 0.622387 0.0424887 . . . . . . . . . . 52260 1 6 . 1 1 7 7 ALA N N 15 . 1 1 7 7 ALA H H 1 0.750954 0.0365708 . . . . . . . . . . 52260 1 7 . 1 1 8 8 LEU N N 15 . 1 1 8 8 LEU H H 1 0.765661 0.0440382 . . . . . . . . . . 52260 1 8 . 1 1 9 9 ILE N N 15 . 1 1 9 9 ILE H H 1 0.831516 0.0376134 . . . . . . . . . . 52260 1 9 . 1 1 13 13 ASP N N 15 . 1 1 13 13 ASP H H 1 0.828881 0.0541982 . . . . . . . . . . 52260 1 10 . 1 1 19 19 VAL N N 15 . 1 1 19 19 VAL H H 1 0.839376 0.0429709 . . . . . . . . . . 52260 1 11 . 1 1 21 21 LYS N N 15 . 1 1 21 21 LYS H H 1 0.854046 0.0663986 . . . . . . . . . . 52260 1 12 . 1 1 22 22 TYR N N 15 . 1 1 22 22 TYR H H 1 0.77731 0.0583881 . . . . . . . . . . 52260 1 13 . 1 1 23 23 VAL N N 15 . 1 1 23 23 VAL H H 1 0.81013 0.048205 . . . . . . . . . . 52260 1 14 . 1 1 24 24 LEU N N 15 . 1 1 24 24 LEU H H 1 0.824838 0.0442842 . . . . . . . . . . 52260 1 15 . 1 1 25 25 ILE N N 15 . 1 1 25 25 ILE H H 1 0.798032 0.0532842 . . . . . . . . . . 52260 1 16 . 1 1 26 26 ARG N N 15 . 1 1 26 26 ARG H H 1 0.826624 0.0545943 . . . . . . . . . . 52260 1 17 . 1 1 27 27 VAL N N 15 . 1 1 27 27 VAL H H 1 0.752569 0.0484331 . . . . . . . . . . 52260 1 18 . 1 1 28 28 HIS N N 15 . 1 1 28 28 HIS H H 1 0.760698 0.0489563 . . . . . . . . . . 52260 1 19 . 1 1 29 29 SER N N 15 . 1 1 29 29 SER H H 1 0.821936 0.0484633 . . . . . . . . . . 52260 1 20 . 1 1 30 30 ALA N N 15 . 1 1 30 30 ALA H H 1 0.646497 0.0379063 . . . . . . . . . . 52260 1 21 . 1 1 32 32 ARG N N 15 . 1 1 32 32 ARG H H 1 0.522673 0.041065 . . . . . . . . . . 52260 1 22 . 1 1 35 35 ALA N N 15 . 1 1 35 35 ALA H H 1 0.446835 0.015932 . . . . . . . . . . 52260 1 23 . 1 1 37 37 ALA N N 15 . 1 1 37 37 ALA H H 1 0.309081 0.0156012 . . . . . . . . . . 52260 1 24 . 1 1 38 38 ALA N N 15 . 1 1 38 38 ALA H H 1 0.362802 0.0202218 . . . . . . . . . . 52260 1 25 . 1 1 39 39 GLU N N 15 . 1 1 39 39 GLU H H 1 0.461534 0.0226684 . . . . . . . . . . 52260 1 26 . 1 1 40 40 SER N N 15 . 1 1 40 40 SER H H 1 0.725499 0.0341042 . . . . . . . . . . 52260 1 27 . 1 1 41 41 LYS N N 15 . 1 1 41 41 LYS H H 1 0.811303 0.0457926 . . . . . . . . . . 52260 1 28 . 1 1 42 42 GLU N N 15 . 1 1 42 42 GLU H H 1 0.738237 0.0505378 . . . . . . . . . . 52260 1 29 . 1 1 43 43 ILE N N 15 . 1 1 43 43 ILE H H 1 0.846588 0.0621279 . . . . . . . . . . 52260 1 30 . 1 1 44 44 VAL N N 15 . 1 1 44 44 VAL H H 1 0.879377 0.067682 . . . . . . . . . . 52260 1 31 . 1 1 45 45 ARG N N 15 . 1 1 45 45 ARG H H 1 0.814353 0.0484563 . . . . . . . . . . 52260 1 32 . 1 1 47 47 TYR N N 15 . 1 1 47 47 TYR H H 1 0.755529 0.0352097 . . . . . . . . . . 52260 1 33 . 1 1 50 50 ALA N N 15 . 1 1 50 50 ALA H H 1 0.906059 0.0359413 . . . . . . . . . . 52260 1 34 . 1 1 51 51 GLU N N 15 . 1 1 51 51 GLU H H 1 0.89243 0.0867285 . . . . . . . . . . 52260 1 35 . 1 1 52 52 TYR N N 15 . 1 1 52 52 TYR H H 1 0.785276 0.0589865 . . . . . . . . . . 52260 1 36 . 1 1 54 54 ALA N N 15 . 1 1 54 54 ALA H H 1 0.917167 0.0654687 . . . . . . . . . . 52260 1 37 . 1 1 55 55 ASP N N 15 . 1 1 55 55 ASP H H 1 0.861366 0.0482105 . . . . . . . . . . 52260 1 38 . 1 1 56 56 ILE N N 15 . 1 1 56 56 ILE H H 1 0.874193 0.0561597 . . . . . . . . . . 52260 1 39 . 1 1 58 58 ASP N N 15 . 1 1 58 58 ASP H H 1 0.792479 0.0393582 . . . . . . . . . . 52260 1 40 . 1 1 59 59 LYS N N 15 . 1 1 59 59 LYS H H 1 0.778886 0.0405222 . . . . . . . . . . 52260 1 41 . 1 1 60 60 VAL N N 15 . 1 1 60 60 VAL H H 1 0.839467 0.0450696 . . . . . . . . . . 52260 1 42 . 1 1 61 61 SER N N 15 . 1 1 61 61 SER H H 1 0.772953 0.0334287 . . . . . . . . . . 52260 1 43 . 1 1 62 62 GLY N N 15 . 1 1 62 62 GLY H H 1 0.768922 0.0385054 . . . . . . . . . . 52260 1 44 . 1 1 63 63 ASP N N 15 . 1 1 63 63 ASP H H 1 0.702072 0.0301688 . . . . . . . . . . 52260 1 45 . 1 1 64 64 MET N N 15 . 1 1 64 64 MET H H 1 0.724303 0.0387818 . . . . . . . . . . 52260 1 46 . 1 1 65 65 GLN N N 15 . 1 1 65 65 GLN H H 1 0.839669 0.0410801 . . . . . . . . . . 52260 1 47 . 1 1 66 66 LYS N N 15 . 1 1 66 66 LYS H H 1 0.790061 0.039564 . . . . . . . . . . 52260 1 48 . 1 1 67 67 GLN N N 15 . 1 1 67 67 GLN H H 1 0.774581 0.0387888 . . . . . . . . . . 52260 1 49 . 1 1 68 68 GLY N N 15 . 1 1 68 68 GLY H H 1 0.713604 0.031374 . . . . . . . . . . 52260 1 50 . 1 1 69 69 CYS N N 15 . 1 1 69 69 CYS H H 1 0.85897 0.0439741 . . . . . . . . . . 52260 1 51 . 1 1 70 70 ASP N N 15 . 1 1 70 70 ASP H H 1 0.704396 0.0401887 . . . . . . . . . . 52260 1 52 . 1 1 71 71 CYS N N 15 . 1 1 71 71 CYS H H 1 0.695855 0.0333402 . . . . . . . . . . 52260 1 53 . 1 1 72 72 GLU N N 15 . 1 1 72 72 GLU H H 1 0.748982 0.0413142 . . . . . . . . . . 52260 1 54 . 1 1 73 73 CYS N N 15 . 1 1 73 73 CYS H H 1 0.803347 0.0737031 . . . . . . . . . . 52260 1 55 . 1 1 74 74 LEU N N 15 . 1 1 74 74 LEU H H 1 0.846072 0.0588123 . . . . . . . . . . 52260 1 56 . 1 1 75 75 GLY N N 15 . 1 1 75 75 GLY H H 1 0.76587 0.0306821 . . . . . . . . . . 52260 1 57 . 1 1 77 77 GLY N N 15 . 1 1 77 77 GLY H H 1 0.986581 0.0620172 . . . . . . . . . . 52260 1 58 . 1 1 78 78 ARG N N 15 . 1 1 78 78 ARG H H 1 0.785642 0.0720788 . . . . . . . . . . 52260 1 59 . 1 1 84 84 GLN N N 15 . 1 1 84 84 GLN H H 1 0.820466 0.0483766 . . . . . . . . . . 52260 1 60 . 1 1 90 90 VAL N N 15 . 1 1 90 90 VAL H H 1 0.822699 0.0646778 . . . . . . . . . . 52260 1 61 . 1 1 91 91 TYR N N 15 . 1 1 91 91 TYR H H 1 0.858763 0.0433468 . . . . . . . . . . 52260 1 62 . 1 1 93 93 TYR N N 15 . 1 1 93 93 TYR H H 1 0.852157 0.0586425 . . . . . . . . . . 52260 1 63 . 1 1 94 94 SER N N 15 . 1 1 94 94 SER H H 1 0.723985 0.0754151 . . . . . . . . . . 52260 1 64 . 1 1 97 97 TYR N N 15 . 1 1 97 97 TYR H H 1 0.814815 0.0604626 . . . . . . . . . . 52260 1 65 . 1 1 98 98 GLY N N 15 . 1 1 98 98 GLY H H 1 0.835352 0.04715 . . . . . . . . . . 52260 1 66 . 1 1 100 100 ALA N N 15 . 1 1 100 100 ALA H H 1 0.857605 0.0501407 . . . . . . . . . . 52260 1 67 . 1 1 101 101 GLN N N 15 . 1 1 101 101 GLN H H 1 0.713467 0.0354231 . . . . . . . . . . 52260 1 68 . 1 1 102 102 HIS N N 15 . 1 1 102 102 HIS H H 1 0.919669 0.04231 . . . . . . . . . . 52260 1 69 . 1 1 103 103 ALA N N 15 . 1 1 103 103 ALA H H 1 0.706091 0.0291642 . . . . . . . . . . 52260 1 70 . 1 1 104 104 ILE N N 15 . 1 1 104 104 ILE H H 1 0.816104 0.0424585 . . . . . . . . . . 52260 1 71 . 1 1 105 105 SER N N 15 . 1 1 105 105 SER H H 1 0.885378 0.0395539 . . . . . . . . . . 52260 1 72 . 1 1 106 106 THR N N 15 . 1 1 106 106 THR H H 1 0.944081 0.0679591 . . . . . . . . . . 52260 1 73 . 1 1 107 107 GLU N N 15 . 1 1 107 107 GLU H H 1 0.898093 0.0358007 . . . . . . . . . . 52260 1 74 . 1 1 108 108 LYS N N 15 . 1 1 108 108 LYS H H 1 0.733631 0.0374164 . . . . . . . . . . 52260 1 75 . 1 1 109 109 ILE N N 15 . 1 1 109 109 ILE H H 1 0.924034 0.0825617 . . . . . . . . . . 52260 1 76 . 1 1 111 111 ALA N N 15 . 1 1 111 111 ALA H H 1 0.927485 0.0595833 . . . . . . . . . . 52260 1 77 . 1 1 112 112 LYS N N 15 . 1 1 112 112 LYS H H 1 0.865084 0.0634853 . . . . . . . . . . 52260 1 78 . 1 1 118 118 VAL N N 15 . 1 1 118 118 VAL H H 1 0.731996 0.092351 . . . . . . . . . . 52260 1 79 . 1 1 119 119 THR N N 15 . 1 1 119 119 THR H H 1 0.953707 0.119901 . . . . . . . . . . 52260 1 80 . 1 1 120 120 TRP N N 15 . 1 1 120 120 TRP H H 1 0.890846 0.0430104 . . . . . . . . . . 52260 1 81 . 1 1 121 121 ALA N N 15 . 1 1 121 121 ALA H H 1 0.732554 0.0600158 . . . . . . . . . . 52260 1 82 . 1 1 122 122 ASN N N 15 . 1 1 122 122 ASN H H 1 0.653301 0.0438549 . . . . . . . . . . 52260 1 83 . 1 1 123 123 ASP N N 15 . 1 1 123 123 ASP H H 1 0.347662 0.0167833 . . . . . . . . . . 52260 1 84 . 1 1 124 124 GLY N N 15 . 1 1 124 124 GLY H H 1 0.484262 0.0236576 . . . . . . . . . . 52260 1 85 . 1 1 125 125 TYR N N 15 . 1 1 125 125 TYR H H 1 0.418307 0.0214954 . . . . . . . . . . 52260 1 stop_ save_ ######################################## # Heteronuclear T1 relaxation values # ######################################## save_heteronucl_T1_relaxation_1 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode heteronucl_T1_relaxation_1 _Heteronucl_T1_list.Entry_ID 52260 _Heteronucl_T1_list.ID 1 _Heteronucl_T1_list.Name 'WT PHPT1 R1 Rates' _Heteronucl_T1_list.Sample_condition_list_ID 1 _Heteronucl_T1_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T1_list.Spectrometer_frequency_1H 700 _Heteronucl_T1_list.T1_coherence_type Sz _Heteronucl_T1_list.T1_val_units s-1 _Heteronucl_T1_list.Details . _Heteronucl_T1_list.Text_data_format . _Heteronucl_T1_list.Text_data . loop_ _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_name _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Sample_state _Heteronucl_T1_experiment.Entry_ID _Heteronucl_T1_experiment.Heteronucl_T1_list_ID 1 'T1/R1 relaxation' . . . 52260 1 stop_ loop_ _Heteronucl_T1_software.Software_ID _Heteronucl_T1_software.Software_label _Heteronucl_T1_software.Method_ID _Heteronucl_T1_software.Method_label _Heteronucl_T1_software.Entry_ID _Heteronucl_T1_software.Heteronucl_T1_list_ID 4 $software_4 . . 52260 1 stop_ loop_ _T1.ID _T1.Assembly_atom_ID _T1.Entity_assembly_ID _T1.Entity_ID _T1.Comp_index_ID _T1.Seq_ID _T1.Comp_ID _T1.Atom_ID _T1.Atom_type _T1.Atom_isotope_number _T1.Val _T1.Val_err _T1.Resonance_ID _T1.Auth_entity_assembly_ID _T1.Auth_seq_ID _T1.Auth_comp_ID _T1.Auth_atom_ID _T1.Entry_ID _T1.Heteronucl_T1_list_ID 1 . 1 1 2 2 ALA N N 15 1.4556 0.0508508 . . . . . 52260 1 2 . 1 1 3 3 VAL N N 15 1.32855 0.0278877 . . . . . 52260 1 3 . 1 1 4 4 ALA N N 15 1.52462 0.052998 . . . . . 52260 1 4 . 1 1 5 5 ASP N N 15 1.20337 0.0419948 . . . . . 52260 1 5 . 1 1 6 6 LEU N N 15 1.15781 0.0550955 . . . . . 52260 1 6 . 1 1 7 7 ALA N N 15 0.992063 0.039466 . . . . . 52260 1 7 . 1 1 8 8 LEU N N 15 0.960615 0.0249151 . . . . . 52260 1 8 . 1 1 9 9 ILE N N 15 1.07817 0.0325484 . . . . . 52260 1 9 . 1 1 12 12 VAL N N 15 1.04932 0.0316007 . . . . . 52260 1 10 . 1 1 13 13 ASP N N 15 1.20005 0.0444996 . . . . . 52260 1 11 . 1 1 14 14 ILE N N 15 1.04932 0.0324815 . . . . . 52260 1 12 . 1 1 19 19 VAL N N 15 1.0798 0.0433738 . . . . . 52260 1 13 . 1 1 21 21 LYS N N 15 1.09135 0.0527629 . . . . . 52260 1 14 . 1 1 22 22 TYR N N 15 1.14811 0.0934566 . . . . . 52260 1 15 . 1 1 23 23 VAL N N 15 1.17096 0.0526521 . . . . . 52260 1 16 . 1 1 24 24 LEU N N 15 1.21907 0.0902076 . . . . . 52260 1 17 . 1 1 25 25 ILE N N 15 1.12803 0.0676946 . . . . . 52260 1 18 . 1 1 26 26 ARG N N 15 1.0321 0.0405851 . . . . . 52260 1 19 . 1 1 27 27 VAL N N 15 1.21021 0.061514 . . . . . 52260 1 20 . 1 1 28 28 HIS N N 15 1.13766 0.0608303 . . . . . 52260 1 21 . 1 1 29 29 SER N N 15 1.06011 0.0424807 . . . . . 52260 1 22 . 1 1 30 30 ALA N N 15 1.30022 0.0507172 . . . . . 52260 1 23 . 1 1 32 32 ARG N N 15 1.26502 0.0526492 . . . . . 52260 1 24 . 1 1 35 35 ALA N N 15 1.38141 0.0498062 . . . . . 52260 1 25 . 1 1 37 37 ALA N N 15 1.33422 0.0651535 . . . . . 52260 1 26 . 1 1 38 38 ALA N N 15 1.2832 0.0447878 . . . . . 52260 1 27 . 1 1 39 39 GLU N N 15 1.23335 0.033009 . . . . . 52260 1 28 . 1 1 40 40 SER N N 15 1.14995 0.0423166 . . . . . 52260 1 29 . 1 1 41 41 LYS N N 15 1.04987 0.0370347 . . . . . 52260 1 30 . 1 1 42 42 GLU N N 15 0.993049 0.0319511 . . . . . 52260 1 31 . 1 1 43 43 ILE N N 15 1.0201 0.0412076 . . . . . 52260 1 32 . 1 1 44 44 VAL N N 15 1.09302 0.0904375 . . . . . 52260 1 33 . 1 1 45 45 ARG N N 15 1.27259 0.0338472 . . . . . 52260 1 34 . 1 1 47 47 TYR N N 15 1.16768 0.0448583 . . . . . 52260 1 35 . 1 1 50 50 ALA N N 15 1.1636 0.0417023 . . . . . 52260 1 36 . 1 1 51 51 GLU N N 15 0.860585 0.0645809 . . . . . 52260 1 37 . 1 1 52 52 TYR N N 15 0.952381 0.0653061 . . . . . 52260 1 38 . 1 1 54 54 ALA N N 15 1.14824 0.0530017 . . . . . 52260 1 39 . 1 1 55 55 ASP N N 15 0.932836 0.0458586 . . . . . 52260 1 40 . 1 1 56 56 ILE N N 15 1.09457 0.0431311 . . . . . 52260 1 41 . 1 1 58 58 ASP N N 15 0.995025 0.0355437 . . . . . 52260 1 42 . 1 1 59 59 LYS N N 15 0.961538 0.0376294 . . . . . 52260 1 43 . 1 1 60 60 VAL N N 15 1.0144 0.0266515 . . . . . 52260 1 44 . 1 1 61 61 SER N N 15 0.995025 0.0417811 . . . . . 52260 1 45 . 1 1 62 62 GLY N N 15 1.03295 0.0423594 . . . . . 52260 1 46 . 1 1 63 63 ASP N N 15 0.940734 0.0300893 . . . . . 52260 1 47 . 1 1 64 64 MET N N 15 0.938967 0.0561617 . . . . . 52260 1 48 . 1 1 65 65 GLN N N 15 1.03413 0.0505834 . . . . . 52260 1 49 . 1 1 66 66 LYS N N 15 0.986193 0.0376387 . . . . . 52260 1 50 . 1 1 67 67 GLN N N 15 1.0009 0.0387698 . . . . . 52260 1 51 . 1 1 68 68 GLY N N 15 1.15835 0.0509871 . . . . . 52260 1 52 . 1 1 69 69 CYS N N 15 1.06655 0.0343536 . . . . . 52260 1 53 . 1 1 70 70 ASP N N 15 1.05307 0.0597733 . . . . . 52260 1 54 . 1 1 71 71 CYS N N 15 1.05753 0.026729 . . . . . 52260 1 55 . 1 1 72 72 GLU N N 15 1.10144 0.0457368 . . . . . 52260 1 56 . 1 1 73 73 CYS N N 15 1.0627 0.0770203 . . . . . 52260 1 57 . 1 1 74 74 LEU N N 15 1.12095 0.0522717 . . . . . 52260 1 58 . 1 1 75 75 GLY N N 15 0.854701 0.0533275 . . . . . 52260 1 59 . 1 1 77 77 GLY N N 15 1.02344 0.103485 . . . . . 52260 1 60 . 1 1 78 78 ARG N N 15 1.03125 0.0902886 . . . . . 52260 1 61 . 1 1 84 84 GLN N N 15 1.03918 0.0556143 . . . . . 52260 1 62 . 1 1 90 90 VAL N N 15 1.13366 0.0420254 . . . . . 52260 1 63 . 1 1 91 91 TYR N N 15 1.15754 0.0296118 . . . . . 52260 1 64 . 1 1 93 93 TYR N N 15 1.01061 0.0338062 . . . . . 52260 1 65 . 1 1 94 94 SER N N 15 1.02712 0.110772 . . . . . 52260 1 66 . 1 1 97 97 TYR N N 15 1.05619 0.0620238 . . . . . 52260 1 67 . 1 1 98 98 GLY N N 15 1.05887 0.0546031 . . . . . 52260 1 68 . 1 1 100 100 ALA N N 15 1.00857 0.0566591 . . . . . 52260 1 69 . 1 1 101 101 GLN N N 15 0.85034 0.026537 . . . . . 52260 1 70 . 1 1 102 102 HIS N N 15 0.976562 0.0214577 . . . . . 52260 1 71 . 1 1 103 103 ALA N N 15 1.23716 0.0439275 . . . . . 52260 1 72 . 1 1 104 104 ILE N N 15 1.17151 0.0425454 . . . . . 52260 1 73 . 1 1 105 105 SER N N 15 1.17233 0.0568987 . . . . . 52260 1 74 . 1 1 106 106 THR N N 15 1.1861 0.0779384 . . . . . 52260 1 75 . 1 1 107 107 GLU N N 15 1.2108 0.0538036 . . . . . 52260 1 76 . 1 1 108 108 LYS N N 15 1.0353 0.0420167 . . . . . 52260 1 77 . 1 1 109 109 ILE N N 15 1.10132 0.046697 . . . . . 52260 1 78 . 1 1 111 111 ALA N N 15 1.10902 0.0477208 . . . . . 52260 1 79 . 1 1 112 112 LYS N N 15 1.16659 0.0242245 . . . . . 52260 1 80 . 1 1 118 118 VAL N N 15 0.912409 0.0457869 . . . . . 52260 1 81 . 1 1 119 119 THR N N 15 1.01978 0.074877 . . . . . 52260 1 82 . 1 1 120 120 TRP N N 15 1.09794 0.0397803 . . . . . 52260 1 83 . 1 1 121 121 ALA N N 15 1.13804 0.053619 . . . . . 52260 1 84 . 1 1 122 122 ASN N N 15 1.01543 0.0784673 . . . . . 52260 1 85 . 1 1 123 123 ASP N N 15 1.25329 0.0537192 . . . . . 52260 1 86 . 1 1 124 124 GLY N N 15 1.33209 0.0351344 . . . . . 52260 1 87 . 1 1 125 125 TYR N N 15 1.29735 0.035514 . . . . . 52260 1 stop_ save_ ######################################## # Heteronuclear T2 relaxation values # ######################################## save_heteronucl_T2_relaxation_1 _Heteronucl_T2_list.Sf_category heteronucl_T2_relaxation _Heteronucl_T2_list.Sf_framecode heteronucl_T2_relaxation_1 _Heteronucl_T2_list.Entry_ID 52260 _Heteronucl_T2_list.ID 1 _Heteronucl_T2_list.Name 'WT PHPT1 R2 Rates' _Heteronucl_T2_list.Sample_condition_list_ID 1 _Heteronucl_T2_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T2_list.Temp_calibration_method methanol _Heteronucl_T2_list.Temp_control_method 'no temperature control applied' _Heteronucl_T2_list.Spectrometer_frequency_1H 700 _Heteronucl_T2_list.T2_coherence_type S(+,-) _Heteronucl_T2_list.T2_val_units s-1 _Heteronucl_T2_list.Rex_units . _Heteronucl_T2_list.Details . _Heteronucl_T2_list.Text_data_format . _Heteronucl_T2_list.Text_data . loop_ _Heteronucl_T2_experiment.Experiment_ID _Heteronucl_T2_experiment.Experiment_name _Heteronucl_T2_experiment.Sample_ID _Heteronucl_T2_experiment.Sample_label _Heteronucl_T2_experiment.Sample_state _Heteronucl_T2_experiment.Entry_ID _Heteronucl_T2_experiment.Heteronucl_T2_list_ID 2 'T2/R2 relaxation' . . . 52260 1 stop_ loop_ _Heteronucl_T2_software.Software_ID _Heteronucl_T2_software.Software_label _Heteronucl_T2_software.Method_ID _Heteronucl_T2_software.Method_label _Heteronucl_T2_software.Entry_ID _Heteronucl_T2_software.Heteronucl_T2_list_ID 4 $software_4 . . 52260 1 stop_ loop_ _T2.ID _T2.Assembly_atom_ID _T2.Entity_assembly_ID _T2.Entity_ID _T2.Comp_index_ID _T2.Seq_ID _T2.Comp_ID _T2.Atom_ID _T2.Atom_type _T2.Atom_isotope_number _T2.T2_val _T2.T2_val_err _T2.Rex_val _T2.Rex_err _T2.Resonance_ID _T2.Auth_entity_assembly_ID _T2.Auth_seq_ID _T2.Auth_comp_ID _T2.Auth_atom_ID _T2.Entry_ID _T2.Heteronucl_T2_list_ID 1 . 1 1 2 2 ALA N N 15 5.40541 0.285756 . . . . . . . 52260 1 2 . 1 1 3 3 VAL N N 15 6.98812 0.264679 . . . . . . . 52260 1 3 . 1 1 4 4 ALA N N 15 8.7184 0.252355 . . . . . . . 52260 1 4 . 1 1 6 6 LEU N N 15 13.6855 0.486962 . . . . . . . 52260 1 5 . 1 1 7 7 ALA N N 15 15.8253 0.378164 . . . . . . . 52260 1 6 . 1 1 8 8 LEU N N 15 13.2153 0.441848 . . . . . . . 52260 1 7 . 1 1 9 9 ILE N N 15 12.4875 0.338385 . . . . . . . 52260 1 8 . 1 1 12 12 VAL N N 15 13.3422 0.396974 . . . . . . . 52260 1 9 . 1 1 13 13 ASP N N 15 13.3085 0.425078 . . . . . . . 52260 1 10 . 1 1 14 14 ILE N N 15 13.3422 0.412995 . . . . . . . 52260 1 11 . 1 1 19 19 VAL N N 15 12.4471 0.390424 . . . . . . . 52260 1 12 . 1 1 21 21 LYS N N 15 13.1961 0.454497 . . . . . . . 52260 1 13 . 1 1 22 22 TYR N N 15 14.6306 0.535135 . . . . . . . 52260 1 14 . 1 1 23 23 VAL N N 15 14.2776 0.426044 . . . . . . . 52260 1 15 . 1 1 24 24 LEU N N 15 14.2837 0.393765 . . . . . . . 52260 1 16 . 1 1 25 25 ILE N N 15 12.41 0.600634 . . . . . . . 52260 1 17 . 1 1 26 26 ARG N N 15 13.2398 0.167929 . . . . . . . 52260 1 18 . 1 1 27 27 VAL N N 15 13.177 0.45839 . . . . . . . 52260 1 19 . 1 1 28 28 HIS N N 15 14.0036 0.517709 . . . . . . . 52260 1 20 . 1 1 29 29 SER N N 15 11.7096 0.349643 . . . . . . . 52260 1 21 . 1 1 30 30 ALA N N 15 10.8213 0.336081 . . . . . . . 52260 1 22 . 1 1 32 32 ARG N N 15 10.0271 0.302632 . . . . . . . 52260 1 23 . 1 1 35 35 ALA N N 15 6.94927 0.221179 . . . . . . . 52260 1 24 . 1 1 37 37 ALA N N 15 6.45578 0.153788 . . . . . . . 52260 1 25 . 1 1 38 38 ALA N N 15 5.80046 0.162507 . . . . . . . 52260 1 26 . 1 1 39 39 GLU N N 15 7.94281 0.256138 . . . . . . . 52260 1 27 . 1 1 40 40 SER N N 15 11.1995 0.267162 . . . . . . . 52260 1 28 . 1 1 41 41 LYS N N 15 13.2398 0.219114 . . . . . . . 52260 1 29 . 1 1 42 42 GLU N N 15 13.0548 0.543667 . . . . . . . 52260 1 30 . 1 1 43 43 ILE N N 15 11.8231 0.329896 . . . . . . . 52260 1 31 . 1 1 44 44 VAL N N 15 14.043 0.352997 . . . . . . . 52260 1 32 . 1 1 45 45 ARG N N 15 12.9016 0.404474 . . . . . . . 52260 1 33 . 1 1 47 47 TYR N N 15 12.8502 0.478868 . . . . . . . 52260 1 34 . 1 1 50 50 ALA N N 15 13.9159 0.464769 . . . . . . . 52260 1 35 . 1 1 51 51 GLU N N 15 13.7495 0.850717 . . . . . . . 52260 1 36 . 1 1 52 52 TYR N N 15 12.7943 0.572927 . . . . . . . 52260 1 37 . 1 1 54 54 ALA N N 15 16.5975 0.641862 . . . . . . . 52260 1 38 . 1 1 55 55 ASP N N 15 14.828 0.369381 . . . . . . . 52260 1 39 . 1 1 56 56 ILE N N 15 14.6864 0.427069 . . . . . . . 52260 1 40 . 1 1 58 58 ASP N N 15 16.2285 0.550431 . . . . . . . 52260 1 41 . 1 1 59 59 LYS N N 15 16.3506 0.387644 . . . . . . . 52260 1 42 . 1 1 60 60 VAL N N 15 16.0617 0.57787 . . . . . . . 52260 1 43 . 1 1 61 61 SER N N 15 15.2648 0.535936 . . . . . . . 52260 1 44 . 1 1 62 62 GLY N N 15 15.9286 0.560725 . . . . . . . 52260 1 45 . 1 1 63 63 ASP N N 15 14.9611 0.452146 . . . . . . . 52260 1 46 . 1 1 64 64 MET N N 15 15.2416 0.538949 . . . . . . . 52260 1 47 . 1 1 65 65 GLN N N 15 15.4059 0.555383 . . . . . . . 52260 1 48 . 1 1 66 66 LYS N N 15 14.8148 0.662826 . . . . . . . 52260 1 49 . 1 1 67 67 GLN N N 15 13.4264 0.414618 . . . . . . . 52260 1 50 . 1 1 68 68 GLY N N 15 12.8816 0.411521 . . . . . . . 52260 1 51 . 1 1 69 69 CYS N N 15 13.4354 0.32492 . . . . . . . 52260 1 52 . 1 1 70 70 ASP N N 15 11.0181 0.347198 . . . . . . . 52260 1 53 . 1 1 71 71 CYS N N 15 12.3031 0.378419 . . . . . . . 52260 1 54 . 1 1 72 72 GLU N N 15 13.1441 0.34035 . . . . . . . 52260 1 55 . 1 1 73 73 CYS N N 15 13.5428 0.502536 . . . . . . . 52260 1 56 . 1 1 74 74 LEU N N 15 12.1109 0.426824 . . . . . . . 52260 1 57 . 1 1 75 75 GLY N N 15 12.4224 0.416651 . . . . . . . 52260 1 58 . 1 1 77 77 GLY N N 15 16.488 0.598082 . . . . . . . 52260 1 59 . 1 1 78 78 ARG N N 15 14.3349 0.649343 . . . . . . . 52260 1 60 . 1 1 84 84 GLN N N 15 13.3351 0.352094 . . . . . . . 52260 1 61 . 1 1 90 90 VAL N N 15 13.4192 0.43038 . . . . . . . 52260 1 62 . 1 1 91 91 TYR N N 15 15.1515 0.40404 . . . . . . . 52260 1 63 . 1 1 93 93 TYR N N 15 15.223 0.783282 . . . . . . . 52260 1 64 . 1 1 94 94 SER N N 15 14.7885 1.52653 . . . . . . . 52260 1 65 . 1 1 97 97 TYR N N 15 15.321 0.837994 . . . . . . . 52260 1 66 . 1 1 98 98 GLY N N 15 13.1944 0.571018 . . . . . . . 52260 1 67 . 1 1 100 100 ALA N N 15 14.3906 0.561209 . . . . . . . 52260 1 68 . 1 1 101 101 GLN N N 15 13.7533 0.603396 . . . . . . . 52260 1 69 . 1 1 102 102 HIS N N 15 15.024 0.320525 . . . . . . . 52260 1 70 . 1 1 103 103 ALA N N 15 11.5527 0.412405 . . . . . . . 52260 1 71 . 1 1 104 104 ILE N N 15 13.4499 0.388935 . . . . . . . 52260 1 72 . 1 1 105 105 SER N N 15 13.0822 0.362823 . . . . . . . 52260 1 73 . 1 1 106 106 THR N N 15 15.2765 0.69078 . . . . . . . 52260 1 74 . 1 1 107 107 GLU N N 15 14.2146 0.327331 . . . . . . . 52260 1 75 . 1 1 108 108 LYS N N 15 12.9383 0.358234 . . . . . . . 52260 1 76 . 1 1 109 109 ILE N N 15 13.0839 0.713852 . . . . . . . 52260 1 77 . 1 1 111 111 ALA N N 15 13.8543 0.472173 . . . . . . . 52260 1 78 . 1 1 112 112 LYS N N 15 12.9651 0.442088 . . . . . . . 52260 1 79 . 1 1 118 118 VAL N N 15 12.7992 0.627427 . . . . . . . 52260 1 80 . 1 1 119 119 THR N N 15 15.1814 0.749045 . . . . . . . 52260 1 81 . 1 1 120 120 TRP N N 15 16.5975 0.476576 . . . . . . . 52260 1 82 . 1 1 121 121 ALA N N 15 14.5688 0.711034 . . . . . . . 52260 1 83 . 1 1 122 122 ASN N N 15 12.9483 0.233047 . . . . . . . 52260 1 84 . 1 1 123 123 ASP N N 15 7.07714 0.184316 . . . . . . . 52260 1 85 . 1 1 124 124 GLY N N 15 8.12348 0.21447 . . . . . . . 52260 1 86 . 1 1 125 125 TYR N N 15 8.22368 0.301625 . . . . . . . 52260 1 stop_ save_