BMRB Entry 52252

Name: 15N13C Chemical Shift Assignment of the NL63 ETM (7-37)
Published: 2024-08-05

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52252

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

15N13C Chemical Shift Assignment of the NL63 ETM (7-37)

Deposition date:

2023-12-28

Original release date:

2024-08-05

Authors:

Pankratova, Yanina; Sucec, Iva; Hong, Mei

Citation:

Citation: Sucec, Iva; Pankratova, Yanina; Parasar, Mriganka; Hong, Mei. "Transmembrane conformation of the envelope protein of an alpha coronavirus, NL63" Protein Sci. 33, e4923-e4923 (2024).
PubMed: 38501465

Assembly members:

Assembly members:
entity_1, polymer, 31 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human alpha-coronavirus, NL63 Taxonomy ID: 693996 Superkingdom: Viruses Kingdom: not available Genus/species: Alphacoronavirus not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: DDNGIVLNSILWLLVMIFFF VLAMTFIKLIQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	140
15N chemical shifts	27

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NL63 ETM (7-37), chain 1	1
2	NL63 ETM (7-37), chain 2	1
3	NL63 ETM (7-37), chain 3	1
4	NL63 ETM (7-37), chain 4	1
5	NL63 ETM (7-37), chain 5	1

Entities:

Entity 1, NL63 ETM (7-37), chain 1 31 residues - Formula weight is not available

1

ASP

ASN

GLY

ILE

VAL

LEU

ASN

SER

ILE

2

LEU

TRP

LEU

VAL

MET

ILE

PHE

3

VAL

LEU

ALA

MET

THR

PHE

ILE

LYS

LEU

ILE

4

GLN

Samples:

sample_1: NL63 ETM (7-37), [U-98% 13C; U-98% 15N], 5.5 mg; sodium chloride 25 mM; H2O 40%; TRIS 25 mM; sodium azide 0.07 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 25 mM; pH: 7.4; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 13C-detected CORD	sample_1	isotropic	sample_conditions_1
2D 13C-detected NCA	sample_1	isotropic	sample_conditions_1
3D NCACX	sample_1	isotropic	sample_conditions_1
3D NCOCX	sample_1	isotropic	sample_conditions_1
3D CONCA	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection, processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE II 800 MHz