data_52252 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 52252 _Entry.Title ; 15N13C Chemical Shift Assignment of the NL63 ETM (7-37) ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2023-12-28 _Entry.Accession_date 2023-12-28 _Entry.Last_release_date 2023-12-28 _Entry.Original_release_date 2023-12-28 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solid-state _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID . _Entry.Generated_software_label . _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details '15N13C backbone and side-chain chemical shift assignment of the transmembrane domain of NL63 E protein' _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Yanina Pankratova . . . 0009-0003-8848-4213 52252 2 Iva Sucec . . . 0000-0002-1192-7800 52252 3 Mei Hong . . . 0000-0001-5255-5858 52252 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 52252 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '13C chemical shifts' 140 52252 '15N chemical shifts' 27 52252 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2024-08-05 . original BMRB . 52252 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 52252 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID 38501465 _Citation.DOI . _Citation.Full_citation . _Citation.Title ; Transmembrane conformation of the envelope protein of an alpha coronavirus, NL63 ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Protein Sci.' _Citation.Journal_name_full . _Citation.Journal_volume 33 _Citation.Journal_issue 4 _Citation.Journal_ASTM . _Citation.Journal_ISSN 1469-896X _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first e4923 _Citation.Page_last e4923 _Citation.Year 2024 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Iva Sucec . . . . 52252 1 2 Yanina Pankratova . . . . 52252 1 3 Mriganka Parasar . . . . 52252 1 4 Mei Hong . . . . 52252 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 52252 _Assembly.ID 1 _Assembly.Name 'NL63 ETM (7-37) putative homopentamer' _Assembly.BMRB_code . _Assembly.Number_of_components 5 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states no _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 'NL63 ETM (7-37), chain 1' 1 $entity_1 . . yes native no no . . . 52252 1 2 'NL63 ETM (7-37), chain 2' 1 $entity_1 . . no native no no . . . 52252 1 3 'NL63 ETM (7-37), chain 3' 1 $entity_1 . . no native no no . . . 52252 1 4 'NL63 ETM (7-37), chain 4' 1 $entity_1 . . no native no no . . . 52252 1 5 'NL63 ETM (7-37), chain 5' 1 $entity_1 . . no native no no . . . 52252 1 stop_ loop_ _Assembly_bio_function.Biological_function _Assembly_bio_function.Entry_ID _Assembly_bio_function.Assembly_ID 'viroporin; viral ion channel' 52252 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 52252 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; DDNGIVLNSILWLLVMIFFF VLAMTFIKLIQ ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq 7-37 _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites yes _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 31 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_biological_function.Biological_function _Entity_biological_function.Entry_ID _Entity_biological_function.Entity_ID 'viroporin forming protein' 52252 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 7 ASP . 52252 1 2 8 ASP . 52252 1 3 9 ASN . 52252 1 4 10 GLY . 52252 1 5 11 ILE . 52252 1 6 12 VAL . 52252 1 7 13 LEU . 52252 1 8 14 ASN . 52252 1 9 15 SER . 52252 1 10 16 ILE . 52252 1 11 17 LEU . 52252 1 12 18 TRP . 52252 1 13 19 LEU . 52252 1 14 20 LEU . 52252 1 15 21 VAL . 52252 1 16 22 MET . 52252 1 17 23 ILE . 52252 1 18 24 PHE . 52252 1 19 25 PHE . 52252 1 20 26 PHE . 52252 1 21 27 VAL . 52252 1 22 28 LEU . 52252 1 23 29 ALA . 52252 1 24 30 MET . 52252 1 25 31 THR . 52252 1 26 32 PHE . 52252 1 27 33 ILE . 52252 1 28 34 LYS . 52252 1 29 35 LEU . 52252 1 30 36 ILE . 52252 1 31 37 GLN . 52252 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . ASP 1 1 52252 1 . ASP 2 2 52252 1 . ASN 3 3 52252 1 . GLY 4 4 52252 1 . ILE 5 5 52252 1 . VAL 6 6 52252 1 . LEU 7 7 52252 1 . ASN 8 8 52252 1 . SER 9 9 52252 1 . ILE 10 10 52252 1 . LEU 11 11 52252 1 . TRP 12 12 52252 1 . LEU 13 13 52252 1 . LEU 14 14 52252 1 . VAL 15 15 52252 1 . MET 16 16 52252 1 . ILE 17 17 52252 1 . PHE 18 18 52252 1 . PHE 19 19 52252 1 . PHE 20 20 52252 1 . VAL 21 21 52252 1 . LEU 22 22 52252 1 . ALA 23 23 52252 1 . MET 24 24 52252 1 . THR 25 25 52252 1 . PHE 26 26 52252 1 . ILE 27 27 52252 1 . LYS 28 28 52252 1 . LEU 29 29 52252 1 . ILE 30 30 52252 1 . GLN 31 31 52252 1 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 52252 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 693996 virus . 'human alpha-coronavirus, NL63' 'human alpha-coronavirus, NL63' . . Viruses . Alphacoronavirus . . . . . . . . . . . . E . 52252 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 52252 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli BL21(DE3) . . plasmid . . pET-SUMO . . 'N-terminal His6 tag, a 6-residue thrombin site, a 98-residue SUMO solubility tag, and the protein of interest' 52252 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 52252 _Sample.ID 1 _Sample.Name 'NL63 ETM (7-37) pH 7.4' _Sample.Type 'proteoliposome sample; hydrated protein in lipid membrane' _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '100% H2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'NL63 ETM (7-37)' '[U-98% 13C; U-98% 15N]' . . 1 $entity_1 . . 5.5 5 6 mg . . . . 52252 1 2 'sodium chloride' 'natural abundance' . . . . . . 25 . . mM . . . . 52252 1 3 H2O 'natural abundance' . . . . . . 40 . . % . . . . 52252 1 4 TRIS 'natural abundance' . . . . . . 25 . . mM . . . . 52252 1 5 'sodium azide' 'natural abundance' . . . . . . 0.07 . . mM . . . . 52252 1 6 EDTA 'natural abundance' . . . . . . 1 . . mM . . . . 52252 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 52252 _Sample_condition_list.ID 1 _Sample_condition_list.Name 'NL63 ETM pH 7.4' _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 25 . mM 52252 1 pH 7.4 . pH 52252 1 temperature 298.15 . K 52252 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 52252 _Software.ID 1 _Software.Type . _Software.Name TOPSPIN _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID collection . 52252 1 processing . 52252 1 stop_ save_ save_software_2 _Software.Sf_category software _Software.Sf_framecode software_2 _Software.Entry_ID 52252 _Software.ID 2 _Software.Type . _Software.Name NMRFAM-SPARKY _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'chemical shift assignment' . 52252 2 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 52252 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name '800 MHz' _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model 'AVANCE II' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 800 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 52252 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 13C-detected CORD' no no no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 52252 1 2 '2D 13C-detected NCA' no no no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 52252 1 3 '3D NCACX' no no no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 52252 1 4 '3D NCOCX' no no no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 52252 1 5 '3D CONCA' no no no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 52252 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chem_shift_reference_1 _Chem_shift_reference.Entry_ID 52252 _Chem_shift_reference.ID 1 _Chem_shift_reference.Name 'NL63 ETM (7-37) pH 7.4' _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID C 13 adamantane 'methyl carbons' . . . . ppm 40.48 external direct 1 . . . . . 52252 1 N 15 N-acetylvaline nitrogen . . . . ppm 122.0 external direct 1 . . . . . 52252 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_1 _Assigned_chem_shift_list.Entry_ID 52252 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Name 'NL63 ETM (7-37) pH 7.4' _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 '2D 13C-detected CORD' . . . 52252 1 2 '2D 13C-detected NCA' . . . 52252 1 3 '3D NCACX' . . . 52252 1 4 '3D NCOCX' . . . 52252 1 5 '3D CONCA' . . . 52252 1 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 1 $software_1 . . 52252 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 . 1 1 1 ASP C C 13 173.2 0.00 . 1 . . . . . 7 D C . 52252 1 2 . 1 . 1 1 1 ASP CA C 13 54 0.00 . 1 . . . . . 7 D CA . 52252 1 3 . 1 . 1 1 1 ASP CB C 13 40.5 0.00 . 1 . . . . . 7 D CB . 52252 1 4 . 1 . 1 2 2 ASP C C 13 177 0.00 . 1 . . . . . 8 D C . 52252 1 5 . 1 . 1 2 2 ASP CA C 13 55.3 0.00 . 1 . . . . . 8 D CA . 52252 1 6 . 1 . 1 2 2 ASP CB C 13 41.7 0.00 . 1 . . . . . 8 D CB . 52252 1 7 . 1 . 1 2 2 ASP N N 15 116.1 0.00 . 1 . . . . . 8 D N . 52252 1 8 . 1 . 1 3 3 ASN C C 13 176.8 0.09 . 1 . . . . . 9 N C . 52252 1 9 . 1 . 1 3 3 ASN CA C 13 54.7 0.07 . 1 . . . . . 9 N CA . 52252 1 10 . 1 . 1 3 3 ASN CB C 13 38.9 0.00 . 1 . . . . . 9 N CB . 52252 1 11 . 1 . 1 3 3 ASN N N 15 118.8 0.08 . 1 . . . . . 9 N N . 52252 1 12 . 1 . 1 4 4 GLY C C 13 176.2 0.18 . 1 . . . . . 10 G C . 52252 1 13 . 1 . 1 4 4 GLY CA C 13 47.7 0.32 . 1 . . . . . 10 G CA . 52252 1 14 . 1 . 1 4 4 GLY N N 15 107.7 0.12 . 1 . . . . . 10 G N . 52252 1 15 . 1 . 1 5 5 ILE C C 13 179 0.20 . 1 . . . . . 11 I C . 52252 1 16 . 1 . 1 5 5 ILE CA C 13 64.4 0.33 . 1 . . . . . 11 I CA . 52252 1 17 . 1 . 1 5 5 ILE CB C 13 38.3 0.22 . 1 . . . . . 11 I CB . 52252 1 18 . 1 . 1 5 5 ILE CG1 C 13 29.12 0.39 . 1 . . . . . 11 I CG1 . 52252 1 19 . 1 . 1 5 5 ILE CG2 C 13 13.6 0.0 . 1 . . . . . 11 I CG2 . 52252 1 20 . 1 . 1 5 5 ILE CD1 C 13 18.04 0.16 . 1 . . . . . 11 I CD1 . 52252 1 21 . 1 . 1 5 5 ILE N N 15 119.4 0.20 . 1 . . . . . 11 I N . 52252 1 22 . 1 . 1 6 6 VAL C C 13 178 0.07 . 1 . . . . . 12 V C . 52252 1 23 . 1 . 1 6 6 VAL CA C 13 67.7 0.17 . 1 . . . . . 12 V CA . 52252 1 24 . 1 . 1 6 6 VAL CB C 13 31.6 0.09 . 1 . . . . . 12 V CB . 52252 1 25 . 1 . 1 6 6 VAL CG1 C 13 23.62 0.08 . 1 . . . . . 12 V CG1 . 52252 1 26 . 1 . 1 6 6 VAL CG2 C 13 22.4 0.25 . 1 . . . . . 12 V CG2 . 52252 1 27 . 1 . 1 6 6 VAL N N 15 122.9 0.56 . 1 . . . . . 12 V N . 52252 1 28 . 1 . 1 7 7 LEU C C 13 178.7 0.53 . 5 . . . . . 13 L C . 52252 1 29 . 1 . 1 7 7 LEU CA C 13 58.7 0.07 . 5 . . . . . 13 L CA . 52252 1 30 . 1 . 1 7 7 LEU CB C 13 41.9 0.20 . 5 . . . . . 13 L CB . 52252 1 31 . 1 . 1 7 7 LEU CG C 13 27.2 0.00 . 5 . . . . . 13 L CG . 52252 1 32 . 1 . 1 8 8 ASN C C 13 178.1 0.35 . 1 . . . . . 14 N C . 52252 1 33 . 1 . 1 8 8 ASN CA C 13 56.9 0.08 . 1 . . . . . 14 N CA . 52252 1 34 . 1 . 1 8 8 ASN CB C 13 38.6 0.37 . 1 . . . . . 14 N CB . 52252 1 35 . 1 . 1 8 8 ASN CG C 13 175.9 0.08 . 1 . . . . . 14 N CG . 52252 1 36 . 1 . 1 8 8 ASN N N 15 116 0.18 . 1 . . . . . 14 N N . 52252 1 37 . 1 . 1 8 8 ASN ND2 N 15 110.1 0.17 . 1 . . . . . 14 N ND . 52252 1 38 . 1 . 1 9 9 SER C C 13 175.8 0.15 . 1 . . . . . 15 S C . 52252 1 39 . 1 . 1 9 9 SER CA C 13 63.6 0.15 . 1 . . . . . 15 S CA . 52252 1 40 . 1 . 1 9 9 SER CB C 13 63.9 0.10 . 1 . . . . . 15 S CB . 52252 1 41 . 1 . 1 9 9 SER N N 15 115.3 0.24 . 1 . . . . . 15 S N . 52252 1 42 . 1 . 1 10 10 ILE C C 13 177.5 0.07 . 1 . . . . . 16 I C . 52252 1 43 . 1 . 1 10 10 ILE CA C 13 66.4 0.11 . 1 . . . . . 16 I CA . 52252 1 44 . 1 . 1 10 10 ILE CB C 13 38.2 0.09 . 1 . . . . . 16 I CB . 52252 1 45 . 1 . 1 10 10 ILE CG1 C 13 31 0.00 . 1 . . . . . 16 I CG1 . 52252 1 46 . 1 . 1 10 10 ILE CG2 C 13 17.6 0.38 . 1 . . . . . 16 I CG2 . 52252 1 47 . 1 . 1 10 10 ILE CD1 C 13 14.8 0.14 . 1 . . . . . 16 I CD1 . 52252 1 48 . 1 . 1 10 10 ILE N N 15 120.9 0.20 . 1 . . . . . 16 I N . 52252 1 49 . 1 . 1 11 11 LEU C C 13 178.7 0.79 . 5 . . . . . 17 L C . 52252 1 50 . 1 . 1 11 11 LEU CA C 13 58.7 0.09 . 5 . . . . . 17 L CA . 52252 1 51 . 1 . 1 11 11 LEU CB C 13 41.9 0.05 . 5 . . . . . 17 L CB . 52252 1 52 . 1 . 1 11 11 LEU CG C 13 27.2 0.04 . 5 . . . . . 17 L CG . 52252 1 53 . 1 . 1 12 12 TRP CA C 13 60.9 0.15 . 1 . . . . . 18 W CA . 52252 1 54 . 1 . 1 12 12 TRP CB C 13 29.6 0.04 . 1 . . . . . 18 W CB . 52252 1 55 . 1 . 1 13 13 LEU C C 13 178.7 0.05 . 5 . . . . . 19 L C . 52252 1 56 . 1 . 1 13 13 LEU CA C 13 58.7 0.09 . 5 . . . . . 19 L CA . 52252 1 57 . 1 . 1 13 13 LEU CB C 13 41.9 0.20 . 5 . . . . . 19 L CB . 52252 1 58 . 1 . 1 13 13 LEU CG C 13 27.2 0.00 . 5 . . . . . 19 L CG . 52252 1 59 . 1 . 1 14 14 LEU C C 13 179.3 0.05 . 5 . . . . . 20 L C . 52252 1 60 . 1 . 1 14 14 LEU CA C 13 58.6 0.09 . 5 . . . . . 20 L CA . 52252 1 61 . 1 . 1 14 14 LEU CB C 13 42.08 0.20 . 5 . . . . . 20 L CB . 52252 1 62 . 1 . 1 14 14 LEU CG C 13 27 0.00 . 5 . . . . . 20 L CG . 52252 1 63 . 1 . 1 14 14 LEU CD1 C 13 27.2 0.00 . 5 . . . . . 20 L CD . 52252 1 64 . 1 . 1 14 14 LEU CD2 C 13 27.2 0.00 . 5 . . . . . 20 L CD . 52252 1 65 . 1 . 1 15 15 VAL C C 13 178.1 0.03 . 1 . . . . . 21 V C . 52252 1 66 . 1 . 1 15 15 VAL CA C 13 67.6 0.11 . 1 . . . . . 21 V CA . 52252 1 67 . 1 . 1 15 15 VAL CB C 13 31.7 0.10 . 1 . . . . . 21 V CB . 52252 1 68 . 1 . 1 15 15 VAL CG1 C 13 22.3 0.26 . 1 . . . . . 21 V CG1 . 52252 1 69 . 1 . 1 15 15 VAL CG2 C 13 23.8 0.00 . 1 . . . . . 21 V CG2 . 52252 1 70 . 1 . 1 15 15 VAL N N 15 119.7 0.04 . 1 . . . . . 21 V N . 52252 1 71 . 1 . 1 16 16 MET C C 13 178.6 0.12 . 1 . . . . . 22 M C . 52252 1 72 . 1 . 1 16 16 MET CA C 13 60.5 0.46 . 1 . . . . . 22 M CA . 52252 1 73 . 1 . 1 16 16 MET CB C 13 33.6 0.13 . 1 . . . . . 22 M CB . 52252 1 74 . 1 . 1 16 16 MET N N 15 115.9 0.09 . 1 . . . . . 22 M N . 52252 1 75 . 1 . 1 17 17 ILE C C 13 177.9 0.12 . 1 . . . . . 23 I C . 52252 1 76 . 1 . 1 17 17 ILE CA C 13 66.4 0.14 . 1 . . . . . 23 I CA . 52252 1 77 . 1 . 1 17 17 ILE CB C 13 38.4 0.00 . 1 . . . . . 23 I CB . 52252 1 78 . 1 . 1 17 17 ILE CG1 C 13 30 0.09 . 1 . . . . . 23 I CG1 . 52252 1 79 . 1 . 1 17 17 ILE CG2 C 13 17.9 0.09 . 1 . . . . . 23 I CG2 . 52252 1 80 . 1 . 1 17 17 ILE CD1 C 13 14.4 0.00 . 1 . . . . . 23 I CD1 . 52252 1 81 . 1 . 1 17 17 ILE N N 15 118.6 0.19 . 1 . . . . . 23 I N . 52252 1 82 . 1 . 1 18 18 PHE C C 13 177.6 0.09 . 5 . . . . . 24 F C . 52252 1 83 . 1 . 1 18 18 PHE CA C 13 62.6 0.17 . 5 . . . . . 24 F CA . 52252 1 84 . 1 . 1 18 18 PHE CB C 13 39.49 0.00 . 5 . . . . . 24 F CB . 52252 1 85 . 1 . 1 18 18 PHE CG C 13 139.4 0.00 . 5 . . . . . 24 F CG . 52252 1 86 . 1 . 1 18 18 PHE CD1 C 13 131.5 0.25 . 5 . . . . . 24 F CD . 52252 1 87 . 1 . 1 18 18 PHE CD2 C 13 131.5 0.25 . 5 . . . . . 24 F CD . 52252 1 88 . 1 . 1 18 18 PHE N N 15 117.5 0.03 . 5 . . . . . 24 F N . 52252 1 89 . 1 . 1 19 19 PHE C C 13 177.6 0.09 . 5 . . . . . 25 F C . 52252 1 90 . 1 . 1 19 19 PHE CA C 13 62.6 0.17 . 5 . . . . . 25 F CA . 52252 1 91 . 1 . 1 19 19 PHE CB C 13 39.49 0.00 . 5 . . . . . 25 F CB . 52252 1 92 . 1 . 1 19 19 PHE CG C 13 139.4 0.00 . 5 . . . . . 25 F CG . 52252 1 93 . 1 . 1 19 19 PHE CD1 C 13 131.5 0.25 . 5 . . . . . 25 F CD . 52252 1 94 . 1 . 1 19 19 PHE CD2 C 13 131.5 0.25 . 5 . . . . . 25 F CD . 52252 1 95 . 1 . 1 19 19 PHE N N 15 117.5 0.03 . 5 . . . . . 25 F N . 52252 1 96 . 1 . 1 20 20 PHE C C 13 177.8 0.09 . 5 . . . . . 26 F C . 52252 1 97 . 1 . 1 20 20 PHE CA C 13 62.6 0.17 . 5 . . . . . 26 F CA . 52252 1 98 . 1 . 1 20 20 PHE CB C 13 39.49 0.00 . 5 . . . . . 26 F CB . 52252 1 99 . 1 . 1 20 20 PHE CG C 13 139.4 0.00 . 5 . . . . . 26 F CG . 52252 1 100 . 1 . 1 20 20 PHE CD1 C 13 131.5 0.25 . 5 . . . . . 26 F CD . 52252 1 101 . 1 . 1 20 20 PHE CD2 C 13 131.5 0.25 . 5 . . . . . 26 F CD . 52252 1 102 . 1 . 1 20 20 PHE N N 15 117.5 0.03 . 5 . . . . . 26 F N . 52252 1 103 . 1 . 1 21 21 VAL C C 13 178.1 0.01 . 1 . . . . . 27 V C . 52252 1 104 . 1 . 1 21 21 VAL CA C 13 67.3 0.14 . 1 . . . . . 27 V CA . 52252 1 105 . 1 . 1 21 21 VAL CB C 13 31.8 0.00 . 1 . . . . . 27 V CB . 52252 1 106 . 1 . 1 21 21 VAL CG1 C 13 22.2 0.00 . 1 . . . . . 27 V CG1 . 52252 1 107 . 1 . 1 21 21 VAL CG2 C 13 23.7 0.00 . 1 . . . . . 27 V CG2 . 52252 1 108 . 1 . 1 21 21 VAL N N 15 117.6 0.06 . 1 . . . . . 27 V N . 52252 1 109 . 1 . 1 22 22 LEU C C 13 178.5 0.13 . 1 . . . . . 28 L C . 52252 1 110 . 1 . 1 22 22 LEU CA C 13 58.7 0.11 . 1 . . . . . 28 L CA . 52252 1 111 . 1 . 1 22 22 LEU CB C 13 42 0.10 . 1 . . . . . 28 L CB . 52252 1 112 . 1 . 1 22 22 LEU CG C 13 27.09 0.18 . 1 . . . . . 28 L CG . 52252 1 113 . 1 . 1 22 22 LEU CD1 C 13 23.4 0.00 . 1 . . . . . 28 L CD . 52252 1 114 . 1 . 1 22 22 LEU CD2 C 13 23.4 0.00 . 1 . . . . . 28 L CD . 52252 1 115 . 1 . 1 23 23 ALA C C 13 179.4 0.07 . 1 . . . . . 29 A C . 52252 1 116 . 1 . 1 23 23 ALA CA C 13 56 0.05 . 1 . . . . . 29 A CA . 52252 1 117 . 1 . 1 23 23 ALA CB C 13 18.4 0.04 . 1 . . . . . 29 A CB . 52252 1 118 . 1 . 1 23 23 ALA N N 15 120.1 0.24 . 1 . . . . . 29 A N . 52252 1 119 . 1 . 1 24 24 MET C C 13 178.8 0.26 . 1 . . . . . 30 M C . 52252 1 120 . 1 . 1 24 24 MET CA C 13 56.5 0.22 . 1 . . . . . 30 M CA . 52252 1 121 . 1 . 1 24 24 MET CB C 13 33 0.09 . 1 . . . . . 30 M CB . 52252 1 122 . 1 . 1 24 24 MET N N 15 112.2 0.22 . 1 . . . . . 30 M N . 52252 1 123 . 1 . 1 25 25 THR C C 13 175.8 0.17 . 1 . . . . . 31 T C . 52252 1 124 . 1 . 1 25 25 THR CA C 13 67.6 0.08 . 1 . . . . . 31 T CA . 52252 1 125 . 1 . 1 25 25 THR CB C 13 68.2 0.16 . 1 . . . . . 31 T CB . 52252 1 126 . 1 . 1 25 25 THR CG2 C 13 22.02 0.27 . 1 . . . . . 31 T CG . 52252 1 127 . 1 . 1 25 25 THR N N 15 116.9 0.18 . 1 . . . . . 31 T N . 52252 1 128 . 1 . 1 26 26 PHE C C 13 177.7 0.37 . 1 . . . . . 32 F C . 52252 1 129 . 1 . 1 26 26 PHE CA C 13 62.9 0.32 . 1 . . . . . 32 F CA . 52252 1 130 . 1 . 1 26 26 PHE CB C 13 39.4 0.35 . 1 . . . . . 32 F CB . 52252 1 131 . 1 . 1 26 26 PHE CD1 C 13 131.5 0.24 . 1 . . . . . 32 F CD . 52252 1 132 . 1 . 1 26 26 PHE CD2 C 13 131.5 0.24 . 1 . . . . . 32 F CD . 52252 1 133 . 1 . 1 26 26 PHE N N 15 119.8 0.13 . 1 . . . . . 32 F N . 52252 1 134 . 1 . 1 27 27 ILE C C 13 177.9 0.13 . 1 . . . . . 33 I C . 52252 1 135 . 1 . 1 27 27 ILE CA C 13 66.2 0.20 . 1 . . . . . 33 I CA . 52252 1 136 . 1 . 1 27 27 ILE CB C 13 38.4 0.02 . 1 . . . . . 33 I CB . 52252 1 137 . 1 . 1 27 27 ILE CG1 C 13 29.9 0.02 . 1 . . . . . 33 I CG1 . 52252 1 138 . 1 . 1 27 27 ILE CG2 C 13 17.8 0.39 . 1 . . . . . 33 I CG2 . 52252 1 139 . 1 . 1 27 27 ILE CD1 C 13 14.4 0.08 . 1 . . . . . 33 I CD1 . 52252 1 140 . 1 . 1 27 27 ILE N N 15 116.4 0.08 . 1 . . . . . 33 I N . 52252 1 141 . 1 . 1 28 28 LYS C C 13 178.6 0.10 . 1 . . . . . 34 K C . 52252 1 142 . 1 . 1 28 28 LYS CA C 13 58.6 0.11 . 1 . . . . . 34 K CA . 52252 1 143 . 1 . 1 28 28 LYS CB C 13 32.3 0.00 . 1 . . . . . 34 K CB . 52252 1 144 . 1 . 1 28 28 LYS CG C 13 26 0.04 . 1 . . . . . 34 K CG . 52252 1 145 . 1 . 1 28 28 LYS CD C 13 29.1 0.12 . 1 . . . . . 34 K CD . 52252 1 146 . 1 . 1 28 28 LYS CE C 13 42.5 0.03 . 1 . . . . . 34 K CE . 52252 1 147 . 1 . 1 28 28 LYS N N 15 117 0.05 . 1 . . . . . 34 K N . 52252 1 148 . 1 . 1 28 28 LYS NZ N 15 39.6 0.00 . 1 . . . . . 34 K NZ . 52252 1 149 . 1 . 1 29 29 LEU C C 13 178.4 0.16 . 1 . . . . . 35 L C . 52252 1 150 . 1 . 1 29 29 LEU CA C 13 57 0.10 . 1 . . . . . 35 L CA . 52252 1 151 . 1 . 1 29 29 LEU CB C 13 42.9 0.16 . 1 . . . . . 35 L CB . 52252 1 152 . 1 . 1 29 29 LEU CG C 13 26.9 0.17 . 1 . . . . . 35 L CG . 52252 1 153 . 1 . 1 29 29 LEU N N 15 116.4 0.17 . 1 . . . . . 35 L N . 52252 1 154 . 1 . 1 30 30 ILE C C 13 175.7 0.11 . 1 . . . . . 36 I C . 52252 1 155 . 1 . 1 30 30 ILE CA C 13 61.7 0.07 . 1 . . . . . 36 I CA . 52252 1 156 . 1 . 1 30 30 ILE CB C 13 38.9 0.10 . 1 . . . . . 36 I CB . 52252 1 157 . 1 . 1 30 30 ILE CG1 C 13 26.9 0.04 . 1 . . . . . 36 I CG1 . 52252 1 158 . 1 . 1 30 30 ILE CG2 C 13 18.1 0.12 . 1 . . . . . 36 I CG2 . 52252 1 159 . 1 . 1 30 30 ILE CD1 C 13 14.8 0.00 . 1 . . . . . 36 I CD1 . 52252 1 160 . 1 . 1 30 30 ILE N N 15 110.5 0.21 . 1 . . . . . 36 I N . 52252 1 161 . 1 . 1 31 31 GLN C C 13 181 0.19 . 1 . . . . . 37 Q C . 52252 1 162 . 1 . 1 31 31 GLN CA C 13 58.2 0.12 . 1 . . . . . 37 Q CA . 52252 1 163 . 1 . 1 31 31 GLN CB C 13 30.5 0.00 . 1 . . . . . 37 Q CB . 52252 1 164 . 1 . 1 31 31 GLN CG C 13 34.9 0.13 . 1 . . . . . 37 Q CG . 52252 1 165 . 1 . 1 31 31 GLN CD C 13 181 0.13 . 1 . . . . . 37 Q CD . 52252 1 166 . 1 . 1 31 31 GLN N N 15 124.7 0.17 . 1 . . . . . 37 Q N . 52252 1 167 . 1 . 1 31 31 GLN NE2 N 15 110.2 0.07 . 1 . . . . . 37 Q NE . 52252 1 stop_ save_