BMRB Entry 52230

Title:
Human Znf706 complexed with cMyc DNA G4Quadruplex heteronuclear NOEs
Deposition date:
2023-12-05
Original release date:
2024-08-28
Authors:
Sahoo, Bikash; Bardwell, James
Citation:

Citation: Sahoo, Bikash; Kocman, Vojc; Clark, Nathan; Myers, Nikhil; Deng, Xiexiong; Wong, Ee; Yang, Harry; Kotar, Anita; Guzman, Bryan; Dominguez, Daniel; Plavec, Janez; Bardwell, James. "Protein G-quadruplex interactions and their effects on phase transitions and protein aggregation"  Nucleic Acids Res. 52, 4702-4722 (2024).
PubMed: 38572746

Assembly members:

Assembly members:
entity_1, polymer, 76 residues, Formula weight is not available
entity_2, polymer, 19 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):

Data sets:
Data typeCount

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Znf7061
2cMyc DNA G4 Quadruplex2

Entities:

Entity 1, Znf706 76 residues - Formula weight is not available

1   METALAARGGLYGLNGLNLYSILEGLNSER
2   GLNGLNLYSASNALALYSLYSGLNALAGLY
3   GLNLYSLYSLYSGLNGLYHISASPGLNLYS
4   ALAALAALALYSALAALALEUILETYRTHR
5   CYSTHRVALCYSARGTHRGLNMETPROASP
6   PROLYSTHRPHELYSGLNHISPHEGLUSER
7   LYSHISPROLYSTHRPROLEUPROPROGLU
8   LEUALAASPVALGLNALA

Entity 2, cMyc DNA G4 Quadruplex 19 residues - Formula weight is not available

1   DTDADGDGDGDADGDGDGDT
2   DADGDGDGDADGDGDGDT

Samples:

sample_1: Human Znf706, [U-100% 15N], 0.2 mM; cMyc DNA G4 quadruplex 0.1 mM; Sodium phosphate buffer 20 mM; KCl 100 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.4; pressure: 1 atm; temperature: 277.1 K

Experiments:

NameSampleSample stateSample conditions
1H-15N heteronoesample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz