data_52230 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 52230 _Entry.Title ; Human Znf706 complexed with cMyc DNA G4Quadruplex heteronuclear NOEs ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2023-12-05 _Entry.Accession_date 2023-12-05 _Entry.Last_release_date 2023-12-05 _Entry.Original_release_date 2023-12-05 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID 1 _Entry.Generated_software_label $software_1 _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Bikash Sahoo . R. . 0000-0002-3683-1178 52230 2 James Bardwell . . . 0000-0003-1683-1944 52230 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID heteronucl_NOEs 2 52230 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2024-08-28 . original BMRB . 52230 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 52229 'Human Znf706 15N-1H heteronuclear NOEs' 52230 BMRB 52231 'Znf706 T1 and T2 relaxation' 52230 BMRB 52232 'Znf706 complex with cMyc DNA G4 qudruplex T1 and T2 relaxation' 52230 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 52230 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID 38572746 _Citation.DOI . _Citation.Full_citation . _Citation.Title ; Protein G-quadruplex interactions and their effects on phase transitions and protein aggregation ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Nucleic Acids Res.' _Citation.Journal_name_full 'Nucleic acids research' _Citation.Journal_volume 52 _Citation.Journal_issue 8 _Citation.Journal_ASTM . _Citation.Journal_ISSN 1362-4962 _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 4702 _Citation.Page_last 4722 _Citation.Year 2024 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Bikash Sahoo B. R. . . 52230 1 2 Vojc Kocman V. . . . 52230 1 3 Nathan Clark N. . . . 52230 1 4 Nikhil Myers N. . . . 52230 1 5 Xiexiong Deng X. . . . 52230 1 6 Ee Wong E. L. . . 52230 1 7 Harry Yang H. J. . . 52230 1 8 Anita Kotar A. . . . 52230 1 9 Bryan Guzman B. B. . . 52230 1 10 Daniel Dominguez D. . . . 52230 1 11 Janez Plavec J. . . . 52230 1 12 James Bardwell . . . . 52230 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 52230 _Assembly.ID 1 _Assembly.Name 'Znf706 complexed with cMyc DNA G4 Quadruplex' _Assembly.BMRB_code . _Assembly.Number_of_components 2 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states no _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 Znf706 1 $entity_1 . . yes native no no . . . 52230 1 2 'cMyc DNA G4 Quadruplex' 2 $entity_2 . . no native no no . . . 52230 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 52230 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MARGQQKIQSQQKNAKKQAG QKKKQGHDQKAAAKAALIYT CTVCRTQMPDPKTFKQHFES KHPKTPLPPELADVQA ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 76 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state unknown _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . MET . 52230 1 2 . ALA . 52230 1 3 . ARG . 52230 1 4 . GLY . 52230 1 5 . GLN . 52230 1 6 . GLN . 52230 1 7 . LYS . 52230 1 8 . ILE . 52230 1 9 . GLN . 52230 1 10 . SER . 52230 1 11 . GLN . 52230 1 12 . GLN . 52230 1 13 . LYS . 52230 1 14 . ASN . 52230 1 15 . ALA . 52230 1 16 . LYS . 52230 1 17 . LYS . 52230 1 18 . GLN . 52230 1 19 . ALA . 52230 1 20 . GLY . 52230 1 21 . GLN . 52230 1 22 . LYS . 52230 1 23 . LYS . 52230 1 24 . LYS . 52230 1 25 . GLN . 52230 1 26 . GLY . 52230 1 27 . HIS . 52230 1 28 . ASP . 52230 1 29 . GLN . 52230 1 30 . LYS . 52230 1 31 . ALA . 52230 1 32 . ALA . 52230 1 33 . ALA . 52230 1 34 . LYS . 52230 1 35 . ALA . 52230 1 36 . ALA . 52230 1 37 . LEU . 52230 1 38 . ILE . 52230 1 39 . TYR . 52230 1 40 . THR . 52230 1 41 . CYS . 52230 1 42 . THR . 52230 1 43 . VAL . 52230 1 44 . CYS . 52230 1 45 . ARG . 52230 1 46 . THR . 52230 1 47 . GLN . 52230 1 48 . MET . 52230 1 49 . PRO . 52230 1 50 . ASP . 52230 1 51 . PRO . 52230 1 52 . LYS . 52230 1 53 . THR . 52230 1 54 . PHE . 52230 1 55 . LYS . 52230 1 56 . GLN . 52230 1 57 . HIS . 52230 1 58 . PHE . 52230 1 59 . GLU . 52230 1 60 . SER . 52230 1 61 . LYS . 52230 1 62 . HIS . 52230 1 63 . PRO . 52230 1 64 . LYS . 52230 1 65 . THR . 52230 1 66 . PRO . 52230 1 67 . LEU . 52230 1 68 . PRO . 52230 1 69 . PRO . 52230 1 70 . GLU . 52230 1 71 . LEU . 52230 1 72 . ALA . 52230 1 73 . ASP . 52230 1 74 . VAL . 52230 1 75 . GLN . 52230 1 76 . ALA . 52230 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 52230 1 . ALA 2 2 52230 1 . ARG 3 3 52230 1 . GLY 4 4 52230 1 . GLN 5 5 52230 1 . GLN 6 6 52230 1 . LYS 7 7 52230 1 . ILE 8 8 52230 1 . GLN 9 9 52230 1 . SER 10 10 52230 1 . GLN 11 11 52230 1 . GLN 12 12 52230 1 . LYS 13 13 52230 1 . ASN 14 14 52230 1 . ALA 15 15 52230 1 . LYS 16 16 52230 1 . LYS 17 17 52230 1 . GLN 18 18 52230 1 . ALA 19 19 52230 1 . GLY 20 20 52230 1 . GLN 21 21 52230 1 . LYS 22 22 52230 1 . LYS 23 23 52230 1 . LYS 24 24 52230 1 . GLN 25 25 52230 1 . GLY 26 26 52230 1 . HIS 27 27 52230 1 . ASP 28 28 52230 1 . GLN 29 29 52230 1 . LYS 30 30 52230 1 . ALA 31 31 52230 1 . ALA 32 32 52230 1 . ALA 33 33 52230 1 . LYS 34 34 52230 1 . ALA 35 35 52230 1 . ALA 36 36 52230 1 . LEU 37 37 52230 1 . ILE 38 38 52230 1 . TYR 39 39 52230 1 . THR 40 40 52230 1 . CYS 41 41 52230 1 . THR 42 42 52230 1 . VAL 43 43 52230 1 . CYS 44 44 52230 1 . ARG 45 45 52230 1 . THR 46 46 52230 1 . GLN 47 47 52230 1 . MET 48 48 52230 1 . PRO 49 49 52230 1 . ASP 50 50 52230 1 . PRO 51 51 52230 1 . LYS 52 52 52230 1 . THR 53 53 52230 1 . PHE 54 54 52230 1 . LYS 55 55 52230 1 . GLN 56 56 52230 1 . HIS 57 57 52230 1 . PHE 58 58 52230 1 . GLU 59 59 52230 1 . SER 60 60 52230 1 . LYS 61 61 52230 1 . HIS 62 62 52230 1 . PRO 63 63 52230 1 . LYS 64 64 52230 1 . THR 65 65 52230 1 . PRO 66 66 52230 1 . LEU 67 67 52230 1 . PRO 68 68 52230 1 . PRO 69 69 52230 1 . GLU 70 70 52230 1 . LEU 71 71 52230 1 . ALA 72 72 52230 1 . ASP 73 73 52230 1 . VAL 74 74 52230 1 . GLN 75 75 52230 1 . ALA 76 76 52230 1 stop_ save_ save_entity_2 _Entity.Sf_category entity _Entity.Sf_framecode entity_2 _Entity.Entry_ID 52230 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name entity_2 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polydeoxyribonucleotide _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; TAGGGAGGGTAGGGAGGGT ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 19 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID 2 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details "cMyc DNA G-quadruplex (5'-TAG GGA GGG TAG GGA GGG T-3')" _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . DT . 52230 2 2 . DA . 52230 2 3 . DG . 52230 2 4 . DG . 52230 2 5 . DG . 52230 2 6 . DA . 52230 2 7 . DG . 52230 2 8 . DG . 52230 2 9 . DG . 52230 2 10 . DT . 52230 2 11 . DA . 52230 2 12 . DG . 52230 2 13 . DG . 52230 2 14 . DG . 52230 2 15 . DA . 52230 2 16 . DG . 52230 2 17 . DG . 52230 2 18 . DG . 52230 2 19 . DT . 52230 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . DT 1 1 52230 2 . DA 2 2 52230 2 . DG 3 3 52230 2 . DG 4 4 52230 2 . DG 5 5 52230 2 . DA 6 6 52230 2 . DG 7 7 52230 2 . DG 8 8 52230 2 . DG 9 9 52230 2 . DT 10 10 52230 2 . DA 11 11 52230 2 . DG 12 12 52230 2 . DG 13 13 52230 2 . DG 14 14 52230 2 . DA 15 15 52230 2 . DG 16 16 52230 2 . DG 17 17 52230 2 . DG 18 18 52230 2 . DT 19 19 52230 2 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 52230 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 52230 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 52230 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . pET28 . . . 52230 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 52230 _Sample.ID 1 _Sample.Name 'Znf706 complexed with cMyc DNA G4 Quadruplex' _Sample.Type solution _Sample.Sub_type . _Sample.Details ; Human Znf706 0.2 mM mixed with 0.1 mM of cMyc DNA G4 Quadruplex. Protein and DNA samples are dissolved in 20 mM Sodium phosphate buffer, pH 7.4, 100 mM KCl, 7.5% D2O. ; _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '92.5% H2O/7.5% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'Human Znf706' '[U-100% 15N]' . . 1 $entity_1 . . 0.2 . . mM . . . . 52230 1 2 'cMyc DNA G4 quadruplex' 'natural abundance' . . 2 $entity_2 . . 0.1 . . mM . . . . 52230 1 3 'Sodium phosphate buffer' 'natural abundance' . . . . . . 20 . . mM . . . . 52230 1 4 KCl 'natural abundance' . . . . . . 100 . . mM . . . . 52230 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 52230 _Sample_condition_list.ID 1 _Sample_condition_list.Name 'Znf706 complexed with cMyc DNA G4 Quadruplex' _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 0.1 . M 52230 1 pH 7.4 . pH 52230 1 pressure 1 . atm 52230 1 temperature 277.1 . K 52230 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 52230 _Software.ID 1 _Software.Type . _Software.Name NMRFAM-SPARKY _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'data analysis' . 52230 1 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 52230 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name 'Bruker 600 MHz w/cryoprobe BioNMR Core University of Michigan' _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model Avance _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 52230 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '1H-15N heteronoe' no no no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 52230 1 stop_ save_ ############################## # Heteronuclear NOE values # ############################## save_heteronucl_NOEs_1 _Heteronucl_NOE_list.Sf_category heteronucl_NOEs _Heteronucl_NOE_list.Sf_framecode heteronucl_NOEs_1 _Heteronucl_NOE_list.Entry_ID 52230 _Heteronucl_NOE_list.ID 1 _Heteronucl_NOE_list.Name 'Znf706 complexed with cMyc DNA G4 Quadruplex' _Heteronucl_NOE_list.Sample_condition_list_ID 1 _Heteronucl_NOE_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_NOE_list.Spectrometer_frequency_1H 600 _Heteronucl_NOE_list.Heteronuclear_NOE_val_type 'peak height' _Heteronucl_NOE_list.NOE_ref_val 0 _Heteronucl_NOE_list.NOE_ref_description . _Heteronucl_NOE_list.Details . _Heteronucl_NOE_list.Text_data_format text _Heteronucl_NOE_list.Text_data ; Assignment w1 w2 Data Height S/N lw1 (hz) lw2 (hz) A2 125.879 8.207 1386256 5 G4 109.854 8.301 1987429 7 Q5 119.815 8.056 2652315 9 Q6 121.489 8.245 2233930 8 K7 123.290 8.236 3576942 12 I8 121.996 8.241 2003733 7 S10 117.311 8.203 1853108 6 Q12 121.317 8.209 7399780 26 N14 119.247 8.233 2289510 8 A15 124.277 8.008 4407568 15 K16 120.301 8.044 4221091 15 K17 122.140 8.316 4038418 14 Q18 122.573 8.067 3295073 11 A19 124.908 7.979 7972808 28 G20 108.085 8.214 2529905 9 Q21 119.550 8.055 4115681 14 K22 122.974 8.186 7262104 25 K23 122.195 8.091 2490459 9 K24 122.575 7.996 2457871 9 Q25 124.861 8.374 3876059 13 G26 110.412 8.319 3550356 12 H27 119.745 7.978 4884222 17 D28 121.136 8.140 3497951 12 Q29 121.354 8.158 9258821 32 A31 125.450 8.188 2231127 8 A32 123.097 8.005 10160700 35 A33 123.106 7.968 9676900 34 K34 120.497 7.951 5760065 20 A35 124.586 7.993 10974510 38 A36 123.039 7.906 6126922 21 L37 121.988 7.854 4447971 15 I38 119.614 7.384 6904762 24 Y39 124.862 8.065 5514201 19 T40 118.841 8.148 7405770 26 C41 128.199 8.431 6051875 21 C44 119.597 7.807 9192174 32 T46 116.129 8.578 5322966 18 Q47 123.140 7.772 8532078 30 M48 120.909 8.285 7462371 26 Q56 117.012 7.840 7804845 27 H57 119.945 8.224 9317771 32 F58 121.350 8.406 1407205 10 E59 113.740 8.056 5821322 20 S60 109.814 7.298 10068853 35 K61 117.357 7.901 8813384 31 H62 122.498 8.717 5911312 21 L67 123.324 8.132 11552556 40 E70 117.412 9.449 4816154 17 L71 118.416 7.571 7558793 26 A72 123.100 7.292 11821868 41 D73 116.461 8.070 13441906 47 V74 120.747 7.293 13290243 46 Q75 126.480 8.324 13363205 46 A76 131.992 7.991 11359703 39 ; loop_ _Heteronucl_NOE_experiment.Experiment_ID _Heteronucl_NOE_experiment.Experiment_name _Heteronucl_NOE_experiment.Sample_ID _Heteronucl_NOE_experiment.Sample_label _Heteronucl_NOE_experiment.Sample_state _Heteronucl_NOE_experiment.Entry_ID _Heteronucl_NOE_experiment.Heteronucl_NOE_list_ID 1 '1H-15N heteronoe' . . . 52230 1 stop_ loop_ _Heteronucl_NOE_software.Software_ID _Heteronucl_NOE_software.Software_label _Heteronucl_NOE_software.Method_ID _Heteronucl_NOE_software.Method_label _Heteronucl_NOE_software.Entry_ID _Heteronucl_NOE_software.Heteronucl_NOE_list_ID 1 $software_1 . . 52230 1 stop_ save_ save_heteronucl_NOEs_2 _Heteronucl_NOE_list.Sf_category heteronucl_NOEs _Heteronucl_NOE_list.Sf_framecode heteronucl_NOEs_2 _Heteronucl_NOE_list.Entry_ID 52230 _Heteronucl_NOE_list.ID 2 _Heteronucl_NOE_list.Name 'Znf706 complexed with cMyc DNA G4 Quadruplex' _Heteronucl_NOE_list.Sample_condition_list_ID 1 _Heteronucl_NOE_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_NOE_list.Spectrometer_frequency_1H 600 _Heteronucl_NOE_list.Heteronuclear_NOE_val_type 'peak height' _Heteronucl_NOE_list.NOE_ref_val 0 _Heteronucl_NOE_list.NOE_ref_description . _Heteronucl_NOE_list.Details . _Heteronucl_NOE_list.Text_data_format text _Heteronucl_NOE_list.Text_data ; Assignment w1 w2 Data Height S/N lw1 (hz) lw2 (hz) A2 125.879 8.207 3765384 13 G4 109.854 8.301 2480561 8 Q5 119.815 8.056 5096659 17 Q6 121.489 8.245 5870956 20 K7 123.290 8.236 8010385 27 I8 121.996 8.241 4140128 14 S10 117.311 8.203 4915807 17 Q12 121.317 8.209 16573276 57 N14 119.247 8.233 5224827 18 A15 124.277 8.008 7358299 25 K16 120.301 8.044 7787350 27 K17 122.140 8.316 9639090 33 Q18 122.573 8.067 6400640 22 A19 124.908 7.979 18113932 62 G20 108.085 8.214 7925612 27 Q21 119.550 8.055 8632923 30 K22 122.974 8.186 13235804 45 K23 122.195 8.091 1744011 6 K24 122.575 7.996 5080952 17 Q25 124.861 8.374 4974867 17 G26 110.412 8.319 9272031 32 H27 119.745 7.978 11509200 39 D28 121.136 8.140 5989097 21 Q29 121.354 8.158 19380900 66 A31 125.450 8.188 6243504 21 A32 123.097 8.005 23430072 80 A33 123.106 7.968 24295402 83 K34 120.497 7.951 20362168 70 A35 124.586 7.993 21985104 75 A36 123.039 7.906 18474016 63 L37 121.988 7.854 11206427 38 I38 119.614 7.384 12693761 43 Y39 124.862 8.065 6808194 23 T40 118.841 8.148 10188794 35 C41 128.199 8.431 6478891 22 C44 119.597 7.807 13254333 45 T46 116.129 8.578 7106034 24 Q47 123.140 7.772 9624759 33 M48 120.909 8.285 9028235 31 Q56 117.012 7.840 9489833 32 H57 119.945 8.224 9741133 33 F58 121.350 8.406 3120693 11 E59 113.740 8.056 8027698 27 S60 109.814 7.298 11911745 41 K61 117.357 7.901 10832438 37 H62 122.498 8.717 7827671 27 L67 123.324 8.132 15627527 54 E70 117.412 9.449 6398232 22 L71 118.416 7.571 9255156 32 A72 123.100 7.292 17946068 61 D73 116.461 8.070 26718862 92 V74 120.747 7.293 28109928 96 Q75 126.480 8.324 38997216 134 A76 131.992 7.991 62675772 215 ; loop_ _Heteronucl_NOE_experiment.Experiment_ID _Heteronucl_NOE_experiment.Experiment_name _Heteronucl_NOE_experiment.Sample_ID _Heteronucl_NOE_experiment.Sample_label _Heteronucl_NOE_experiment.Sample_state _Heteronucl_NOE_experiment.Entry_ID _Heteronucl_NOE_experiment.Heteronucl_NOE_list_ID 1 '1H-15N heteronoe' . . . 52230 2 stop_ loop_ _Heteronucl_NOE_software.Software_ID _Heteronucl_NOE_software.Software_label _Heteronucl_NOE_software.Method_ID _Heteronucl_NOE_software.Method_label _Heteronucl_NOE_software.Entry_ID _Heteronucl_NOE_software.Heteronucl_NOE_list_ID 1 $software_1 . . 52230 2 stop_ save_