BMRB Entry 5161

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5161

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Title:
Characterization of the ATP-binding Domain of the Sarco(endo)plasmic Reticulum Ca (2+) -ATPase: Probing Nucleotide Binding By Multidimensional NMR
Deposition date:
2001-09-25
Original release date:
2001-10-17
Authors:
Abu-Abed, Mona; Mal, Tapas; Kainosho, Masatsune; MacLennan, David; Ikura, Mitsuhiko
Citation:

Citation: Abu-Abed, Mona; Mal, Tapas; Kainosho, Masatsune; MacLennan, David; Ikura, Mitsuhiko. "Characterization of the ATP-binding Domain of the Sarco(endo)plasmic Reticulum Ca(2+)-ATPase: Probing Nucleotide Binding By Multidimensional NMR "  Biochemistry 41, 1156-1164 (2002).
PubMed: 11802714

Assembly members:

Assembly members:
Nucleotide Binding Domain of the Sarco(endo)plasmic reticulum Ca2+-ATPase, polymer, 247 residues, 27467.52 Da.

Natural source:

Natural source:   Common Name: Rabbit   Taxonomy ID: 9986   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oryctolagus cuniculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Eschirechia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nucleotide Binding Domain of the Sarco(endo)plasmic reticulum Ca2+-ATPase: SMHTTNQMSVCKMFIIDKVD GDFCSLNEFSITGSTYAPEG EVLKNDKPIRSGQFDGLVEL ATICALCNDSSLDFNETKGV YEKVGEATETALTTLVEKMN VFNTEVRNLSKVERANACNS VIRQLMKKEFTLEFSRDRKS MSVYCSPAKSSRAAVGNKMF VKGAPEGVIDRCNYVRVGTT RVPMTGPVKEKILSVIKEWG TGRDTLRCLALATRDTPPKR EEMVLDDSSRFMEYETDLTF VGVVGML

Data sets:
Data typeCount
1H chemical shifts222
13C chemical shifts447
15N chemical shifts222

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SERCA1a(357-600)1

Entities:

Entity 1, SERCA1a(357-600) 247 residues - 27467.52 Da.

1   SERMETHISTHRTHRASNGLNMETSERVAL
2   CYSLYSMETPHEILEILEASPLYSVALASP
3   GLYASPPHECYSSERLEUASNGLUPHESER
4   ILETHRGLYSERTHRTYRALAPROGLUGLY
5   GLUVALLEULYSASNASPLYSPROILEARG
6   SERGLYGLNPHEASPGLYLEUVALGLULEU
7   ALATHRILECYSALALEUCYSASNASPSER
8   SERLEUASPPHEASNGLUTHRLYSGLYVAL
9   TYRGLULYSVALGLYGLUALATHRGLUTHR
10   ALALEUTHRTHRLEUVALGLULYSMETASN
11   VALPHEASNTHRGLUVALARGASNLEUSER
12   LYSVALGLUARGALAASNALACYSASNSER
13   VALILEARGGLNLEUMETLYSLYSGLUPHE
14   THRLEUGLUPHESERARGASPARGLYSSER
15   METSERVALTYRCYSSERPROALALYSSER
16   SERARGALAALAVALGLYASNLYSMETPHE
17   VALLYSGLYALAPROGLUGLYVALILEASP
18   ARGCYSASNTYRVALARGVALGLYTHRTHR
19   ARGVALPROMETTHRGLYPROVALLYSGLU
20   LYSILELEUSERVALILELYSGLUTRPGLY
21   THRGLYARGASPTHRLEUARGCYSLEUALA
22   LEUALATHRARGASPTHRPROPROLYSARG
23   GLUGLUMETVALLEUASPASPSERSERARG
24   PHEMETGLUTYRGLUTHRASPLEUTHRPHE
25   VALGLYVALVALGLYMETLEU

Samples:

sample_1: Nucleotide Binding Domain of the Sarco(endo)plasmic reticulum Ca2+-ATPase, [U-99% 2H; U-100% 13C; U-100% 15N], 1.25 mM

sample_2: Nucleotide Binding Domain of the Sarco(endo)plasmic reticulum Ca2+-ATPase, [U-100% 15N], 1.0 mM

condition_1: pH: 7.5; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HN(CO)CACBnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 17119
PDB
GB AAA31165 ABW96358
REF NP_001082787
SP P04191
AlphaFold P04191

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks