BMRB Entry 17119
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17119
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NMR-STAR v3 text file.
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Citation: Buffy, Jarrod; Buck-Koehntop, Bethany; Porcelli, Fernando; Traaseth, Nathaniel; Veglia, Gianluigi; Thomas, David. "Defining the Intramembrane Binding Mechanism of Sarcolipin to Calcium ATPase USing Solution NMR Spectroscopy" J. Mol. Biol. 358, 420-429 (2006).
PubMed: 16519897
Assembly members:
SLN, polymer, 31 residues, Formula weight is not available
SERCA, polymer, 1001 residues, Formula weight is not available
Natural source: Common Name: Rabbit Taxonomy ID: 9986 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oryctolagus cuniculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: na
Entity Sequences (FASTA):
SLN: MERSTRELCLNFTVVLITVI
LIWLLVRSYQY
SERCA: MEAAHSKSTEECLAYFGVSE
TTGLTPDQVKRHLEKYGHNE
LPAEEGKSLWELVIEQFEDL
LVRILLLAACISFVLAWFEE
GEETITAFVEPFVILLILIA
NAIVGVWQERNAENAIEALK
EYEPEMGKVYRADRKSVQRI
KARDIVPGDIVEVAVGDKVP
ADIRILSIKSTTLRVDQSIL
TGESVSVIKHTEPVPDPRAV
NQDKKNMLFSGTNIAAGKAL
GIVATTGVSTEIGKIRDQMA
ATEQDKTPLQQKLDEFGEQL
SKVISLICVAVWLINIGHFN
DPVHGGSWIRGAIYYFKIAV
ALAVAAIPEGLPAVITTCLA
LGTRRMAKKNAIVRSLPSVE
TLGCTSVICSDKTGTLTTNQ
MSVCKMFIIDKVDGDFCSLN
EFSITGSTYAPEGEVLKNDK
PIRSGQFDGLVELATICALC
NDSSLDFNETKGVYEKVGEA
TETALTTLVEKMNVFNTEVR
NLSKVERANACNSVIRQLMK
KEFTLEFSRDRKSMSVYCSP
AKSSRAAVGNKMFVKGAPEG
VIDRCNYVRVGTTRVPMTGP
VKEKILSVIKEWGTGRDTLR
CLALATRDTPPKREEMVLDD
SSRFMEYETDLTFVGVVGML
DPPRKEVMGSIQLCRDAGIR
VIMITGDNKGTAIAICRRIG
IFGENEEVADRAYTGREFDD
LPLAEQREACRRACCFARVE
PSHKSKIVEYLQSYDEITAM
TGDGVNDAPALKKAEIGIAM
GSGTAVAKTASEMVLADDNF
STIVAAVEEGRAIYNNMKQF
IRYLISSNVGEVVCIFLTAA
LGLPEALIPVQLLWVNLVTD
GLPATALGFNPPDLDIMDRP
PRSPKEPLISGWLFFRYMAI
GGYVGAATVGAAAWWFMYAE
DGPGVTYHQLTHFMQCTEDH
PHFEGLDCEIFEAPEPMTMA
LSVLVTIEMCNALNSLSENQ
SLMRMPPWVNIWLLGSICLS
MSLHFLILYVDPLPMIFKLK
ALDLTQWLMVLKISLPVIGL
DEILKFIARNYLEDPEDERR
K
- binding_constants
| Data type | Count |
| binding constants | 1 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SLN | 1 |
| 2 | SERCA | 2 |
Entities:
Entity 1, SLN 31 residues - Formula weight is not available
| 1 | MET | GLU | ARG | SER | THR | ARG | GLU | LEU | CYS | LEU | ||||
| 2 | ASN | PHE | THR | VAL | VAL | LEU | ILE | THR | VAL | ILE | ||||
| 3 | LEU | ILE | TRP | LEU | LEU | VAL | ARG | SER | TYR | GLN | ||||
| 4 | TYR |
Entity 2, SERCA 1001 residues - Formula weight is not available
| 1 | MET | GLU | ALA | ALA | HIS | SER | LYS | SER | THR | GLU | ||||
| 2 | GLU | CYS | LEU | ALA | TYR | PHE | GLY | VAL | SER | GLU | ||||
| 3 | THR | THR | GLY | LEU | THR | PRO | ASP | GLN | VAL | LYS | ||||
| 4 | ARG | HIS | LEU | GLU | LYS | TYR | GLY | HIS | ASN | GLU | ||||
| 5 | LEU | PRO | ALA | GLU | GLU | GLY | LYS | SER | LEU | TRP | ||||
| 6 | GLU | LEU | VAL | ILE | GLU | GLN | PHE | GLU | ASP | LEU | ||||
| 7 | LEU | VAL | ARG | ILE | LEU | LEU | LEU | ALA | ALA | CYS | ||||
| 8 | ILE | SER | PHE | VAL | LEU | ALA | TRP | PHE | GLU | GLU | ||||
| 9 | GLY | GLU | GLU | THR | ILE | THR | ALA | PHE | VAL | GLU | ||||
| 10 | PRO | PHE | VAL | ILE | LEU | LEU | ILE | LEU | ILE | ALA | ||||
| 11 | ASN | ALA | ILE | VAL | GLY | VAL | TRP | GLN | GLU | ARG | ||||
| 12 | ASN | ALA | GLU | ASN | ALA | ILE | GLU | ALA | LEU | LYS | ||||
| 13 | GLU | TYR | GLU | PRO | GLU | MET | GLY | LYS | VAL | TYR | ||||
| 14 | ARG | ALA | ASP | ARG | LYS | SER | VAL | GLN | ARG | ILE | ||||
| 15 | LYS | ALA | ARG | ASP | ILE | VAL | PRO | GLY | ASP | ILE | ||||
| 16 | VAL | GLU | VAL | ALA | VAL | GLY | ASP | LYS | VAL | PRO | ||||
| 17 | ALA | ASP | ILE | ARG | ILE | LEU | SER | ILE | LYS | SER | ||||
| 18 | THR | THR | LEU | ARG | VAL | ASP | GLN | SER | ILE | LEU | ||||
| 19 | THR | GLY | GLU | SER | VAL | SER | VAL | ILE | LYS | HIS | ||||
| 20 | THR | GLU | PRO | VAL | PRO | ASP | PRO | ARG | ALA | VAL | ||||
| 21 | ASN | GLN | ASP | LYS | LYS | ASN | MET | LEU | PHE | SER | ||||
| 22 | GLY | THR | ASN | ILE | ALA | ALA | GLY | LYS | ALA | LEU | ||||
| 23 | GLY | ILE | VAL | ALA | THR | THR | GLY | VAL | SER | THR | ||||
| 24 | GLU | ILE | GLY | LYS | ILE | ARG | ASP | GLN | MET | ALA | ||||
| 25 | ALA | THR | GLU | GLN | ASP | LYS | THR | PRO | LEU | GLN | ||||
| 26 | GLN | LYS | LEU | ASP | GLU | PHE | GLY | GLU | GLN | LEU | ||||
| 27 | SER | LYS | VAL | ILE | SER | LEU | ILE | CYS | VAL | ALA | ||||
| 28 | VAL | TRP | LEU | ILE | ASN | ILE | GLY | HIS | PHE | ASN | ||||
| 29 | ASP | PRO | VAL | HIS | GLY | GLY | SER | TRP | ILE | ARG | ||||
| 30 | GLY | ALA | ILE | TYR | TYR | PHE | LYS | ILE | ALA | VAL | ||||
| 31 | ALA | LEU | ALA | VAL | ALA | ALA | ILE | PRO | GLU | GLY | ||||
| 32 | LEU | PRO | ALA | VAL | ILE | THR | THR | CYS | LEU | ALA | ||||
| 33 | LEU | GLY | THR | ARG | ARG | MET | ALA | LYS | LYS | ASN | ||||
| 34 | ALA | ILE | VAL | ARG | SER | LEU | PRO | SER | VAL | GLU | ||||
| 35 | THR | LEU | GLY | CYS | THR | SER | VAL | ILE | CYS | SER | ||||
| 36 | ASP | LYS | THR | GLY | THR | LEU | THR | THR | ASN | GLN | ||||
| 37 | MET | SER | VAL | CYS | LYS | MET | PHE | ILE | ILE | ASP | ||||
| 38 | LYS | VAL | ASP | GLY | ASP | PHE | CYS | SER | LEU | ASN | ||||
| 39 | GLU | PHE | SER | ILE | THR | GLY | SER | THR | TYR | ALA | ||||
| 40 | PRO | GLU | GLY | GLU | VAL | LEU | LYS | ASN | ASP | LYS | ||||
| 41 | PRO | ILE | ARG | SER | GLY | GLN | PHE | ASP | GLY | LEU | ||||
| 42 | VAL | GLU | LEU | ALA | THR | ILE | CYS | ALA | LEU | CYS | ||||
| 43 | ASN | ASP | SER | SER | LEU | ASP | PHE | ASN | GLU | THR | ||||
| 44 | LYS | GLY | VAL | TYR | GLU | LYS | VAL | GLY | GLU | ALA | ||||
| 45 | THR | GLU | THR | ALA | LEU | THR | THR | LEU | VAL | GLU | ||||
| 46 | LYS | MET | ASN | VAL | PHE | ASN | THR | GLU | VAL | ARG | ||||
| 47 | ASN | LEU | SER | LYS | VAL | GLU | ARG | ALA | ASN | ALA | ||||
| 48 | CYS | ASN | SER | VAL | ILE | ARG | GLN | LEU | MET | LYS | ||||
| 49 | LYS | GLU | PHE | THR | LEU | GLU | PHE | SER | ARG | ASP | ||||
| 50 | ARG | LYS | SER | MET | SER | VAL | TYR | CYS | SER | PRO | ||||
| 51 | ALA | LYS | SER | SER | ARG | ALA | ALA | VAL | GLY | ASN | ||||
| 52 | LYS | MET | PHE | VAL | LYS | GLY | ALA | PRO | GLU | GLY | ||||
| 53 | VAL | ILE | ASP | ARG | CYS | ASN | TYR | VAL | ARG | VAL | ||||
| 54 | GLY | THR | THR | ARG | VAL | PRO | MET | THR | GLY | PRO | ||||
| 55 | VAL | LYS | GLU | LYS | ILE | LEU | SER | VAL | ILE | LYS | ||||
| 56 | GLU | TRP | GLY | THR | GLY | ARG | ASP | THR | LEU | ARG | ||||
| 57 | CYS | LEU | ALA | LEU | ALA | THR | ARG | ASP | THR | PRO | ||||
| 58 | PRO | LYS | ARG | GLU | GLU | MET | VAL | LEU | ASP | ASP | ||||
| 59 | SER | SER | ARG | PHE | MET | GLU | TYR | GLU | THR | ASP | ||||
| 60 | LEU | THR | PHE | VAL | GLY | VAL | VAL | GLY | MET | LEU | ||||
| 61 | ASP | PRO | PRO | ARG | LYS | GLU | VAL | MET | GLY | SER | ||||
| 62 | ILE | GLN | LEU | CYS | ARG | ASP | ALA | GLY | ILE | ARG | ||||
| 63 | VAL | ILE | MET | ILE | THR | GLY | ASP | ASN | LYS | GLY | ||||
| 64 | THR | ALA | ILE | ALA | ILE | CYS | ARG | ARG | ILE | GLY | ||||
| 65 | ILE | PHE | GLY | GLU | ASN | GLU | GLU | VAL | ALA | ASP | ||||
| 66 | ARG | ALA | TYR | THR | GLY | ARG | GLU | PHE | ASP | ASP | ||||
| 67 | LEU | PRO | LEU | ALA | GLU | GLN | ARG | GLU | ALA | CYS | ||||
| 68 | ARG | ARG | ALA | CYS | CYS | PHE | ALA | ARG | VAL | GLU | ||||
| 69 | PRO | SER | HIS | LYS | SER | LYS | ILE | VAL | GLU | TYR | ||||
| 70 | LEU | GLN | SER | TYR | ASP | GLU | ILE | THR | ALA | MET | ||||
| 71 | THR | GLY | ASP | GLY | VAL | ASN | ASP | ALA | PRO | ALA | ||||
| 72 | LEU | LYS | LYS | ALA | GLU | ILE | GLY | ILE | ALA | MET | ||||
| 73 | GLY | SER | GLY | THR | ALA | VAL | ALA | LYS | THR | ALA | ||||
| 74 | SER | GLU | MET | VAL | LEU | ALA | ASP | ASP | ASN | PHE | ||||
| 75 | SER | THR | ILE | VAL | ALA | ALA | VAL | GLU | GLU | GLY | ||||
| 76 | ARG | ALA | ILE | TYR | ASN | ASN | MET | LYS | GLN | PHE | ||||
| 77 | ILE | ARG | TYR | LEU | ILE | SER | SER | ASN | VAL | GLY | ||||
| 78 | GLU | VAL | VAL | CYS | ILE | PHE | LEU | THR | ALA | ALA | ||||
| 79 | LEU | GLY | LEU | PRO | GLU | ALA | LEU | ILE | PRO | VAL | ||||
| 80 | GLN | LEU | LEU | TRP | VAL | ASN | LEU | VAL | THR | ASP | ||||
| 81 | GLY | LEU | PRO | ALA | THR | ALA | LEU | GLY | PHE | ASN | ||||
| 82 | PRO | PRO | ASP | LEU | ASP | ILE | MET | ASP | ARG | PRO | ||||
| 83 | PRO | ARG | SER | PRO | LYS | GLU | PRO | LEU | ILE | SER | ||||
| 84 | GLY | TRP | LEU | PHE | PHE | ARG | TYR | MET | ALA | ILE | ||||
| 85 | GLY | GLY | TYR | VAL | GLY | ALA | ALA | THR | VAL | GLY | ||||
| 86 | ALA | ALA | ALA | TRP | TRP | PHE | MET | TYR | ALA | GLU | ||||
| 87 | ASP | GLY | PRO | GLY | VAL | THR | TYR | HIS | GLN | LEU | ||||
| 88 | THR | HIS | PHE | MET | GLN | CYS | THR | GLU | ASP | HIS | ||||
| 89 | PRO | HIS | PHE | GLU | GLY | LEU | ASP | CYS | GLU | ILE | ||||
| 90 | PHE | GLU | ALA | PRO | GLU | PRO | MET | THR | MET | ALA | ||||
| 91 | LEU | SER | VAL | LEU | VAL | THR | ILE | GLU | MET | CYS | ||||
| 92 | ASN | ALA | LEU | ASN | SER | LEU | SER | GLU | ASN | GLN | ||||
| 93 | SER | LEU | MET | ARG | MET | PRO | PRO | TRP | VAL | ASN | ||||
| 94 | ILE | TRP | LEU | LEU | GLY | SER | ILE | CYS | LEU | SER | ||||
| 95 | MET | SER | LEU | HIS | PHE | LEU | ILE | LEU | TYR | VAL | ||||
| 96 | ASP | PRO | LEU | PRO | MET | ILE | PHE | LYS | LEU | LYS | ||||
| 97 | ALA | LEU | ASP | LEU | THR | GLN | TRP | LEU | MET | VAL | ||||
| 98 | LEU | LYS | ILE | SER | LEU | PRO | VAL | ILE | GLY | LEU | ||||
| 99 | ASP | GLU | ILE | LEU | LYS | PHE | ILE | ALA | ARG | ASN | ||||
| 100 | TYR | LEU | GLU | ASP | PRO | GLU | ASP | GLU | ARG | ARG | ||||
| 101 | LYS |