BMRB Entry 17119

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17119

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Title:
Defining the Intramembrane Binding Mechanism of Sarcolipin to Calcium ATPase USing Solution NMR Spectroscopy
Deposition date:
2010-08-11
Original release date:
2010-11-10
Authors:
Buffy, Jarrod; Buck-Koehntop, Bethany; Porcelli, Fernando; Traaseth, Nathaniel; Veglia, Gianluigi; Thomas, David
Citation:

Citation: Buffy, Jarrod; Buck-Koehntop, Bethany; Porcelli, Fernando; Traaseth, Nathaniel; Veglia, Gianluigi; Thomas, David. "Defining the Intramembrane Binding Mechanism of Sarcolipin to Calcium ATPase USing Solution NMR Spectroscopy"  J. Mol. Biol. 358, 420-429 (2006).
PubMed: 16519897

Assembly members:

Assembly members:
SLN, polymer, 31 residues, Formula weight is not available
SERCA, polymer, 1001 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rabbit   Taxonomy ID: 9986   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oryctolagus cuniculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: na

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SLN: MERSTRELCLNFTVVLITVI LIWLLVRSYQY
SERCA: MEAAHSKSTEECLAYFGVSE TTGLTPDQVKRHLEKYGHNE LPAEEGKSLWELVIEQFEDL LVRILLLAACISFVLAWFEE GEETITAFVEPFVILLILIA NAIVGVWQERNAENAIEALK EYEPEMGKVYRADRKSVQRI KARDIVPGDIVEVAVGDKVP ADIRILSIKSTTLRVDQSIL TGESVSVIKHTEPVPDPRAV NQDKKNMLFSGTNIAAGKAL GIVATTGVSTEIGKIRDQMA ATEQDKTPLQQKLDEFGEQL SKVISLICVAVWLINIGHFN DPVHGGSWIRGAIYYFKIAV ALAVAAIPEGLPAVITTCLA LGTRRMAKKNAIVRSLPSVE TLGCTSVICSDKTGTLTTNQ MSVCKMFIIDKVDGDFCSLN EFSITGSTYAPEGEVLKNDK PIRSGQFDGLVELATICALC NDSSLDFNETKGVYEKVGEA TETALTTLVEKMNVFNTEVR NLSKVERANACNSVIRQLMK KEFTLEFSRDRKSMSVYCSP AKSSRAAVGNKMFVKGAPEG VIDRCNYVRVGTTRVPMTGP VKEKILSVIKEWGTGRDTLR CLALATRDTPPKREEMVLDD SSRFMEYETDLTFVGVVGML DPPRKEVMGSIQLCRDAGIR VIMITGDNKGTAIAICRRIG IFGENEEVADRAYTGREFDD LPLAEQREACRRACCFARVE PSHKSKIVEYLQSYDEITAM TGDGVNDAPALKKAEIGIAM GSGTAVAKTASEMVLADDNF STIVAAVEEGRAIYNNMKQF IRYLISSNVGEVVCIFLTAA LGLPEALIPVQLLWVNLVTD GLPATALGFNPPDLDIMDRP PRSPKEPLISGWLFFRYMAI GGYVGAATVGAAAWWFMYAE DGPGVTYHQLTHFMQCTEDH PHFEGLDCEIFEAPEPMTMA LSVLVTIEMCNALNSLSENQ SLMRMPPWVNIWLLGSICLS MSLHFLILYVDPLPMIFKLK ALDLTQWLMVLKISLPVIGL DEILKFIARNYLEDPEDERR K

Data sets:
  • binding_constants
Data typeCount
binding constants1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SLN1
2SERCA2

Entities:

Entity 1, SLN 31 residues - Formula weight is not available

1   METGLUARGSERTHRARGGLULEUCYSLEU
2   ASNPHETHRVALVALLEUILETHRVALILE
3   LEUILETRPLEULEUVALARGSERTYRGLN
4   TYR

Entity 2, SERCA 1001 residues - Formula weight is not available

1   METGLUALAALAHISSERLYSSERTHRGLU
2   GLUCYSLEUALATYRPHEGLYVALSERGLU
3   THRTHRGLYLEUTHRPROASPGLNVALLYS
4   ARGHISLEUGLULYSTYRGLYHISASNGLU
5   LEUPROALAGLUGLUGLYLYSSERLEUTRP
6   GLULEUVALILEGLUGLNPHEGLUASPLEU
7   LEUVALARGILELEULEULEUALAALACYS
8   ILESERPHEVALLEUALATRPPHEGLUGLU
9   GLYGLUGLUTHRILETHRALAPHEVALGLU
10   PROPHEVALILELEULEUILELEUILEALA
11   ASNALAILEVALGLYVALTRPGLNGLUARG
12   ASNALAGLUASNALAILEGLUALALEULYS
13   GLUTYRGLUPROGLUMETGLYLYSVALTYR
14   ARGALAASPARGLYSSERVALGLNARGILE
15   LYSALAARGASPILEVALPROGLYASPILE
16   VALGLUVALALAVALGLYASPLYSVALPRO
17   ALAASPILEARGILELEUSERILELYSSER
18   THRTHRLEUARGVALASPGLNSERILELEU
19   THRGLYGLUSERVALSERVALILELYSHIS
20   THRGLUPROVALPROASPPROARGALAVAL
21   ASNGLNASPLYSLYSASNMETLEUPHESER
22   GLYTHRASNILEALAALAGLYLYSALALEU
23   GLYILEVALALATHRTHRGLYVALSERTHR
24   GLUILEGLYLYSILEARGASPGLNMETALA
25   ALATHRGLUGLNASPLYSTHRPROLEUGLN
26   GLNLYSLEUASPGLUPHEGLYGLUGLNLEU
27   SERLYSVALILESERLEUILECYSVALALA
28   VALTRPLEUILEASNILEGLYHISPHEASN
29   ASPPROVALHISGLYGLYSERTRPILEARG
30   GLYALAILETYRTYRPHELYSILEALAVAL
31   ALALEUALAVALALAALAILEPROGLUGLY
32   LEUPROALAVALILETHRTHRCYSLEUALA
33   LEUGLYTHRARGARGMETALALYSLYSASN
34   ALAILEVALARGSERLEUPROSERVALGLU
35   THRLEUGLYCYSTHRSERVALILECYSSER
36   ASPLYSTHRGLYTHRLEUTHRTHRASNGLN
37   METSERVALCYSLYSMETPHEILEILEASP
38   LYSVALASPGLYASPPHECYSSERLEUASN
39   GLUPHESERILETHRGLYSERTHRTYRALA
40   PROGLUGLYGLUVALLEULYSASNASPLYS
41   PROILEARGSERGLYGLNPHEASPGLYLEU
42   VALGLULEUALATHRILECYSALALEUCYS
43   ASNASPSERSERLEUASPPHEASNGLUTHR
44   LYSGLYVALTYRGLULYSVALGLYGLUALA
45   THRGLUTHRALALEUTHRTHRLEUVALGLU
46   LYSMETASNVALPHEASNTHRGLUVALARG
47   ASNLEUSERLYSVALGLUARGALAASNALA
48   CYSASNSERVALILEARGGLNLEUMETLYS
49   LYSGLUPHETHRLEUGLUPHESERARGASP
50   ARGLYSSERMETSERVALTYRCYSSERPRO
51   ALALYSSERSERARGALAALAVALGLYASN
52   LYSMETPHEVALLYSGLYALAPROGLUGLY
53   VALILEASPARGCYSASNTYRVALARGVAL
54   GLYTHRTHRARGVALPROMETTHRGLYPRO
55   VALLYSGLULYSILELEUSERVALILELYS
56   GLUTRPGLYTHRGLYARGASPTHRLEUARG
57   CYSLEUALALEUALATHRARGASPTHRPRO
58   PROLYSARGGLUGLUMETVALLEUASPASP
59   SERSERARGPHEMETGLUTYRGLUTHRASP
60   LEUTHRPHEVALGLYVALVALGLYMETLEU
61   ASPPROPROARGLYSGLUVALMETGLYSER
62   ILEGLNLEUCYSARGASPALAGLYILEARG
63   VALILEMETILETHRGLYASPASNLYSGLY
64   THRALAILEALAILECYSARGARGILEGLY
65   ILEPHEGLYGLUASNGLUGLUVALALAASP
66   ARGALATYRTHRGLYARGGLUPHEASPASP
67   LEUPROLEUALAGLUGLNARGGLUALACYS
68   ARGARGALACYSCYSPHEALAARGVALGLU
69   PROSERHISLYSSERLYSILEVALGLUTYR
70   LEUGLNSERTYRASPGLUILETHRALAMET
71   THRGLYASPGLYVALASNASPALAPROALA
72   LEULYSLYSALAGLUILEGLYILEALAMET
73   GLYSERGLYTHRALAVALALALYSTHRALA
74   SERGLUMETVALLEUALAASPASPASNPHE
75   SERTHRILEVALALAALAVALGLUGLUGLY
76   ARGALAILETYRASNASNMETLYSGLNPHE
77   ILEARGTYRLEUILESERSERASNVALGLY
78   GLUVALVALCYSILEPHELEUTHRALAALA
79   LEUGLYLEUPROGLUALALEUILEPROVAL
80   GLNLEULEUTRPVALASNLEUVALTHRASP
81   GLYLEUPROALATHRALALEUGLYPHEASN
82   PROPROASPLEUASPILEMETASPARGPRO
83   PROARGSERPROLYSGLUPROLEUILESER
84   GLYTRPLEUPHEPHEARGTYRMETALAILE
85   GLYGLYTYRVALGLYALAALATHRVALGLY
86   ALAALAALATRPTRPPHEMETTYRALAGLU
87   ASPGLYPROGLYVALTHRTYRHISGLNLEU
88   THRHISPHEMETGLNCYSTHRGLUASPHIS
89   PROHISPHEGLUGLYLEUASPCYSGLUILE
90   PHEGLUALAPROGLUPROMETTHRMETALA
91   LEUSERVALLEUVALTHRILEGLUMETCYS
92   ASNALALEUASNSERLEUSERGLUASNGLN
93   SERLEUMETARGMETPROPROTRPVALASN
94   ILETRPLEULEUGLYSERILECYSLEUSER
95   METSERLEUHISPHELEUILELEUTYRVAL
96   ASPPROLEUPROMETILEPHELYSLEULYS
97   ALALEUASPLEUTHRGLNTRPLEUMETVAL
98   LEULYSILESERLEUPROVALILEGLYLEU
99   ASPGLUILELEULYSPHEILEALAARGASN
100   TYRLEUGLUASPPROGLUASPGLUARGARG
101   LYS

Samples:

sample_1: SLN, [U-15N], 0.3 mM; phosphate-buffered saline 20 mM; DPC 300 mM; SERCA0 – 56 mg/ml; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.0; pressure: ambient atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAB86979 ABD98462 AAA31165 AAH37354 ABW96358 EDM17472 ELW66399
REF NP_001038031 NP_001075856 NP_001193294 XP_004320742 XP_005903864 NP_001082787 XP_004586901 XP_004586902 XP_005621437 XP_006148309
SP P42532 P04191
TPG DAA22460
AlphaFold P42532 P04191