BMRB Entry 5159

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5159
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Sequence-specific resonance assignment of the second Ran-binding domain of human RanBP2
Deposition date:
2001-09-25
Original release date:
2005-04-13
Authors:
Doker, Rolf; Geyer, Peter; Zhao, Xiaodong; Kremer, Werner; Villa Braslavsky, Carolina; Kuhlmann, Jurgen; Kalbitzer, Hans Robert
Citation:

Citation: Doker, Rolf; Zhao, Xiaodong; Kremer, Werner; Villa Braslavsky, Carolina; Kuhlmann, Jurgen; Kalbitzer, Hans. "Letter to the Editor: Sequence-specific Resonance Assignment of the Second Ran-binding Domain of Human RanBP2"  J. Biomol. NMR 22, 185-186 (2002).
PubMed: 11883781

Assembly members:

Assembly members:
Ran-binding domain 2 of Ran-binding protein 2, polymer, 130 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ran-binding domain 2 of Ran-binding protein 2: GSGEEDEKVLYSQRVKLFRF DAEVSQWKERGLGNLKILKN EVNGKLRMLMRREQVLKVCA NHWITTTMNLKPLSGSDRAW MWLASDFSDGDAKLEQLAAK FKTPELAEEFKQKFEECQRL LLDIPLQTPK

Data sets:
Data typeCount
13C chemical shifts452
15N chemical shifts133
1H chemical shifts723

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RanBP2-RanBD21

Entities:

Entity 1, RanBP2-RanBD2 130 residues - Formula weight is not available

1   GLYSERGLYGLUGLUASPGLULYSVALLEU
2   TYRSERGLNARGVALLYSLEUPHEARGPHE
3   ASPALAGLUVALSERGLNTRPLYSGLUARG
4   GLYLEUGLYASNLEULYSILELEULYSASN
5   GLUVALASNGLYLYSLEUARGMETLEUMET
6   ARGARGGLUGLNVALLEULYSVALCYSALA
7   ASNHISTRPILETHRTHRTHRMETASNLEU
8   LYSPROLEUSERGLYSERASPARGALATRP
9   METTRPLEUALASERASPPHESERASPGLY
10   ASPALALYSLEUGLUGLNLEUALAALALYS
11   PHELYSTHRPROGLULEUALAGLUGLUPHE
12   LYSGLNLYSPHEGLUGLUCYSGLNARGLEU
13   LEULEUASPILEPROLEUGLNTHRPROLYS

Samples:

sample_1: Ran-binding domain 2 of Ran-binding protein 2, [U-13C; U-15N], 0.8 mM

sample_2: Ran-binding domain 2 of Ran-binding protein 2, [U-13C; U-15N], 0.7 mM; D2O 100%

sample_3: Ran-binding domain 2 of Ran-binding protein 2, [U-15N], 1.1 mM

ex-cond_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availableex-cond_1
HN(CO)CAnot availablenot availableex-cond_1
CBCA(CO)NHnot availablenot availableex-cond_1
CBCANHnot availablenot availableex-cond_1
HBHA(CO)NHnot availablenot availableex-cond_1
HNCOnot availablenot availableex-cond_1
1H-15N NOESYnot availablenot availableex-cond_1
1H-15N TOCSYnot availablenot availableex-cond_1
HCCH-TOCSYnot availablenot availableex-cond_1

Software:

AUREMOL -

AURELIA -

xwinnmr -

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 16459
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks