BMRB Entry 51068

Name: FUS RGG2 (372-422)
Published: 2021-09-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51068

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

FUS RGG2 (372-422)

Deposition date:

2021-08-25

Original release date:

2021-09-19

Authors:

Murthy, Anastasia; Fawzi, Nicolas

Citation:

Citation: Murthy, Anastasia; Tang, Wai Shing; Jovic, Nina; Janke, Abigail; Seo, Da Hee; Perdikari, Theodora Myrto; Mittal, Jeetain; Fawzi, Nicolas. "Molecular interactions contributing to FUS SYGQ LC-RGG phase separation and co-partitioning with RNA polymerase II heptads" Nat. Struct. Mol. Biol. 28, 923-935 (2021).
PubMed: 34759379

Assembly members:

Assembly members:
entity_1, polymer, 51 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTHMT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: RADFNRGGGNGRGGRGRGGP MGRGGYGGGGSGGGGRGGFP SGGGGGGGQQR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	89
15N chemical shifts	29
1H chemical shifts	29

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FUS RGG2	1

Entities:

Entity 1, FUS RGG2 51 residues - Formula weight is not available

1

ARG

ALA

ASP

PHE

ASN

ARG

GLY

ASN

2

GLY

ARG

GLY

ARG

GLY

ARG

GLY

PRO

3

MET

GLY

ARG

GLY

TYR

GLY

4

SER

GLY

ARG

GLY

PHE

PRO

5

SER

GLY

GLN

6

ARG

Samples:

sample_1: FUS RGG2 (372-422), [U-99% 13C; U-99% 15N], 0.95 uM; MES 20 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks