data_51068 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 51068 _Entry.Title ; FUS RGG2 (372-422) ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2021-08-25 _Entry.Accession_date 2021-08-25 _Entry.Last_release_date 2021-08-25 _Entry.Original_release_date 2021-08-25 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID 1 _Entry.Generated_software_label $software_1 _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details 'Backbone chemical shift assignments for FUS RGG2 (372-422)' _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Anastasia Murthy . C. . 0000-0002-2490-697X 51068 2 Nicolas Fawzi . L. . 0000-0001-5483-0577 51068 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 51068 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '13C chemical shifts' 89 51068 '15N chemical shifts' 29 51068 '1H chemical shifts' 29 51068 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 2 . . 2022-01-29 2021-08-25 update BMRB 'update entry citation' 51068 1 . . 2021-09-19 2021-08-25 original author 'original release' 51068 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 51067 'Backbone Chemical Shift Assignments for the RGG1 domain of Fused in Sarcoma (FUS 164-267)' 51068 BMRB 51069 'Backbone chemical shift assignments for FUS RGG3 (453-507)' 51068 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 51068 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID 34759379 _Citation.DOI . _Citation.Full_citation . _Citation.Title ; Molecular interactions contributing to FUS SYGQ LC-RGG phase separation and co-partitioning with RNA polymerase II heptads ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Nat. Struct. Mol. Biol.' _Citation.Journal_name_full 'Nature structural & molecular biology' _Citation.Journal_volume 28 _Citation.Journal_issue 11 _Citation.Journal_ASTM . _Citation.Journal_ISSN 1545-9985 _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 923 _Citation.Page_last 935 _Citation.Year 2021 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Anastasia Murthy A. C. . . 51068 1 2 'Wai Shing' Tang W. S. . . 51068 1 3 Nina Jovic N. . . . 51068 1 4 Abigail Janke A. M. . . 51068 1 5 'Da Hee' Seo D. H. . . 51068 1 6 'Theodora Myrto' Perdikari T. M. . . 51068 1 7 Jeetain Mittal J. . . . 51068 1 8 Nicolas Fawzi N. L. . . 51068 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 51068 _Assembly.ID 1 _Assembly.Name 'FUS RGG2' _Assembly.BMRB_code . _Assembly.Number_of_components 1 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states no _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 'FUS RGG2' 1 $entity_1 . . yes native no no . . . 51068 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 51068 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; RADFNRGGGNGRGGRGRGGP MGRGGYGGGGSGGGGRGGFP SGGGGGGGQQR ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states yes _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 51 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'all free' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 372 ARG . 51068 1 2 373 ALA . 51068 1 3 374 ASP . 51068 1 4 375 PHE . 51068 1 5 376 ASN . 51068 1 6 377 ARG . 51068 1 7 378 GLY . 51068 1 8 379 GLY . 51068 1 9 380 GLY . 51068 1 10 381 ASN . 51068 1 11 382 GLY . 51068 1 12 383 ARG . 51068 1 13 384 GLY . 51068 1 14 385 GLY . 51068 1 15 386 ARG . 51068 1 16 387 GLY . 51068 1 17 388 ARG . 51068 1 18 389 GLY . 51068 1 19 390 GLY . 51068 1 20 391 PRO . 51068 1 21 392 MET . 51068 1 22 393 GLY . 51068 1 23 394 ARG . 51068 1 24 395 GLY . 51068 1 25 396 GLY . 51068 1 26 397 TYR . 51068 1 27 398 GLY . 51068 1 28 399 GLY . 51068 1 29 400 GLY . 51068 1 30 401 GLY . 51068 1 31 402 SER . 51068 1 32 403 GLY . 51068 1 33 404 GLY . 51068 1 34 405 GLY . 51068 1 35 406 GLY . 51068 1 36 407 ARG . 51068 1 37 408 GLY . 51068 1 38 409 GLY . 51068 1 39 410 PHE . 51068 1 40 411 PRO . 51068 1 41 412 SER . 51068 1 42 413 GLY . 51068 1 43 414 GLY . 51068 1 44 415 GLY . 51068 1 45 416 GLY . 51068 1 46 417 GLY . 51068 1 47 418 GLY . 51068 1 48 419 GLY . 51068 1 49 420 GLN . 51068 1 50 421 GLN . 51068 1 51 422 ARG . 51068 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . ARG 1 1 51068 1 . ALA 2 2 51068 1 . ASP 3 3 51068 1 . PHE 4 4 51068 1 . ASN 5 5 51068 1 . ARG 6 6 51068 1 . GLY 7 7 51068 1 . GLY 8 8 51068 1 . GLY 9 9 51068 1 . ASN 10 10 51068 1 . GLY 11 11 51068 1 . ARG 12 12 51068 1 . GLY 13 13 51068 1 . GLY 14 14 51068 1 . ARG 15 15 51068 1 . GLY 16 16 51068 1 . ARG 17 17 51068 1 . GLY 18 18 51068 1 . GLY 19 19 51068 1 . PRO 20 20 51068 1 . MET 21 21 51068 1 . GLY 22 22 51068 1 . ARG 23 23 51068 1 . GLY 24 24 51068 1 . GLY 25 25 51068 1 . TYR 26 26 51068 1 . GLY 27 27 51068 1 . GLY 28 28 51068 1 . GLY 29 29 51068 1 . GLY 30 30 51068 1 . SER 31 31 51068 1 . GLY 32 32 51068 1 . GLY 33 33 51068 1 . GLY 34 34 51068 1 . GLY 35 35 51068 1 . ARG 36 36 51068 1 . GLY 37 37 51068 1 . GLY 38 38 51068 1 . PHE 39 39 51068 1 . PRO 40 40 51068 1 . SER 41 41 51068 1 . GLY 42 42 51068 1 . GLY 43 43 51068 1 . GLY 44 44 51068 1 . GLY 45 45 51068 1 . GLY 46 46 51068 1 . GLY 47 47 51068 1 . GLY 48 48 51068 1 . GLN 49 49 51068 1 . GLN 50 50 51068 1 . ARG 51 51 51068 1 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 51068 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 51068 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 51068 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . pTHMT . . . 51068 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 51068 _Sample.ID 1 _Sample.Name 'FUS RGG2' _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'FUS RGG2 (372-422)' '[U-99% 13C; U-99% 15N]' . . 1 $entity_1 . . 0.95 . . uM . . . . 51068 1 2 MES 'natural abundance' . . . . . . 20 . . mM . . . . 51068 1 3 'sodium chloride' 'natural abundance' . . . . . . 150 . . mM . . . . 51068 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 51068 _Sample_condition_list.ID 1 _Sample_condition_list.Name 'FUS RGG2' _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 150 . mM 51068 1 pH 5.5 . pH 51068 1 pressure 1 . atm 51068 1 temperature 298 . K 51068 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 51068 _Software.ID 1 _Software.Type . _Software.Name NMRFAM-SPARKY _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'chemical shift assignment' . 51068 1 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 51068 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name '850 MHz Bruker Avance III HD' _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model 'AVANCE III' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 850 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 51068 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '3D CBCA(CO)NH' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 51068 1 2 '3D HNCO' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 51068 1 3 '3D HN(CA)CO' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 51068 1 4 '3D HNCACB' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 51068 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chem_shift_reference_1 _Chem_shift_reference.Entry_ID 51068 _Chem_shift_reference.ID 1 _Chem_shift_reference.Name 'FUS RGG2' _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID C 13 TSP 'methyl carbons' . . . . ppm 117.081 external direct 1 . . . . . 51068 1 H 1 water protons . . . . ppm 4.773 internal direct 1 . . . . . 51068 1 N 15 'ammonium chloride' nitrogen . . . . ppm 42.75 external direct 1 . . . . . 51068 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_1 _Assigned_chem_shift_list.Entry_ID 51068 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Name 'FUS RGG2' _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 '3D CBCA(CO)NH' . . . 51068 1 2 '3D HNCO' . . . 51068 1 3 '3D HN(CA)CO' . . . 51068 1 4 '3D HNCACB' . . . 51068 1 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 1 $software_1 . . 51068 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 . 1 1 1 ARG H H 1 8.409 0.00 . . . . . . . 372 ARG HN . 51068 1 2 . 1 . 1 1 1 ARG C C 13 173.304 0.01 . . . . . . . 372 ARG C . 51068 1 3 . 1 . 1 1 1 ARG CA C 13 53.563 0.06 . . . . . . . 372 ARG CA . 51068 1 4 . 1 . 1 1 1 ARG CB C 13 28.184 0.02 . . . . . . . 372 ARG CB . 51068 1 5 . 1 . 1 1 1 ARG N N 15 123.200 0.00 . . . . . . . 372 ARG N . 51068 1 6 . 1 . 1 2 2 ALA H H 1 8.354 0.00 . . . . . . . 373 ALA HN . 51068 1 7 . 1 . 1 2 2 ALA C C 13 174.390 0.01 . . . . . . . 373 ALA C . 51068 1 8 . 1 . 1 2 2 ALA CA C 13 49.842 0.02 . . . . . . . 373 ALA CA . 51068 1 9 . 1 . 1 2 2 ALA N N 15 125.612 0.01 . . . . . . . 373 ALA N . 51068 1 10 . 1 . 1 3 3 ASP H H 1 8.171 0.00 . . . . . . . 374 ASP HN . 51068 1 11 . 1 . 1 3 3 ASP C C 13 173.437 0.01 . . . . . . . 374 ASP C . 51068 1 12 . 1 . 1 3 3 ASP CA C 13 51.200 0.01 . . . . . . . 374 ASP CA . 51068 1 13 . 1 . 1 3 3 ASP CB C 13 38.255 0.01 . . . . . . . 374 ASP CB . 51068 1 14 . 1 . 1 3 3 ASP N N 15 118.941 0.00 . . . . . . . 374 ASP N . 51068 1 15 . 1 . 1 4 4 PHE H H 1 7.984 0.00 . . . . . . . 375 PHE HN . 51068 1 16 . 1 . 1 4 4 PHE C C 13 172.898 0.00 . . . . . . . 375 PHE C . 51068 1 17 . 1 . 1 4 4 PHE CA C 13 55.254 0.03 . . . . . . . 375 PHE CA . 51068 1 18 . 1 . 1 4 4 PHE CB C 13 36.582 0.06 . . . . . . . 375 PHE CB . 51068 1 19 . 1 . 1 4 4 PHE N N 15 120.560 0.02 . . . . . . . 375 PHE N . 51068 1 20 . 1 . 1 5 5 ASN H H 1 8.286 0.00 . . . . . . . 376 ASN HN . 51068 1 21 . 1 . 1 5 5 ASN C C 13 172.458 0.01 . . . . . . . 376 ASN C . 51068 1 22 . 1 . 1 5 5 ASN CA C 13 50.590 0.06 . . . . . . . 376 ASN CA . 51068 1 23 . 1 . 1 5 5 ASN CB C 13 35.926 0.01 . . . . . . . 376 ASN CB . 51068 1 24 . 1 . 1 5 5 ASN N N 15 120.036 0.00 . . . . . . . 376 ASN N . 51068 1 25 . 1 . 1 6 6 ARG H H 1 8.131 0.00 . . . . . . . 377 ARG HN . 51068 1 26 . 1 . 1 6 6 ARG C C 13 174.176 0.00 . . . . . . . 377 ARG C . 51068 1 27 . 1 . 1 6 6 ARG CA C 13 53.724 0.01 . . . . . . . 377 ARG CA . 51068 1 28 . 1 . 1 6 6 ARG CB C 13 27.728 0.00 . . . . . . . 377 ARG CB . 51068 1 29 . 1 . 1 6 6 ARG N N 15 121.506 0.01 . . . . . . . 377 ARG N . 51068 1 30 . 1 . 1 7 7 GLY H H 1 8.321 0.00 . . . . . . . 378 GLY HN . 51068 1 31 . 1 . 1 7 7 GLY C C 13 171.976 0.02 . . . . . . . 378 GLY C . 51068 1 32 . 1 . 1 7 7 GLY CA C 13 42.593 0.04 . . . . . . . 378 GLY CA . 51068 1 33 . 1 . 1 7 7 GLY N N 15 109.449 0.01 . . . . . . . 378 GLY N . 51068 1 34 . 1 . 1 8 8 GLY H H 1 8.181 0.00 . . . . . . . 379 GLY HN . 51068 1 35 . 1 . 1 8 8 GLY C C 13 172.100 0.01 . . . . . . . 379 GLY C . 51068 1 36 . 1 . 1 8 8 GLY CA C 13 42.561 0.01 . . . . . . . 379 GLY CA . 51068 1 37 . 1 . 1 8 8 GLY N N 15 108.481 0.01 . . . . . . . 379 GLY N . 51068 1 38 . 1 . 1 9 9 GLY H H 1 8.247 0.00 . . . . . . . 380 GLY HN . 51068 1 39 . 1 . 1 9 9 GLY C C 13 171.513 0.00 . . . . . . . 380 GLY C . 51068 1 40 . 1 . 1 9 9 GLY CA C 13 42.580 0.05 . . . . . . . 380 GLY CA . 51068 1 41 . 1 . 1 9 9 GLY N N 15 108.573 0.02 . . . . . . . 380 GLY N . 51068 1 42 . 1 . 1 10 10 ASN H H 1 8.335 0.00 . . . . . . . 381 ASN HN . 51068 1 43 . 1 . 1 10 10 ASN C C 13 173.257 0.00 . . . . . . . 381 ASN C . 51068 1 44 . 1 . 1 10 10 ASN CA C 13 50.518 0.07 . . . . . . . 381 ASN CA . 51068 1 45 . 1 . 1 10 10 ASN CB C 13 36.087 0.00 . . . . . . . 381 ASN CB . 51068 1 46 . 1 . 1 10 10 ASN N N 15 118.362 0.01 . . . . . . . 381 ASN N . 51068 1 47 . 1 . 1 11 11 GLY H H 1 8.372 0.00 . . . . . . . 382 GLY HN . 51068 1 48 . 1 . 1 11 11 GLY C C 13 171.748 0.00 . . . . . . . 382 GLY C . 51068 1 49 . 1 . 1 11 11 GLY CA C 13 42.861 0.04 . . . . . . . 382 GLY CA . 51068 1 50 . 1 . 1 11 11 GLY N N 15 108.986 0.01 . . . . . . . 382 GLY N . 51068 1 51 . 1 . 1 12 12 ARG H H 1 8.141 0.00 . . . . . . . 383 ARG HN . 51068 1 52 . 1 . 1 12 12 ARG C C 13 174.318 0.00 . . . . . . . 383 ARG C . 51068 1 53 . 1 . 1 12 12 ARG CA C 13 53.617 0.00 . . . . . . . 383 ARG CA . 51068 1 54 . 1 . 1 12 12 ARG CB C 13 27.857 0.00 . . . . . . . 383 ARG CB . 51068 1 55 . 1 . 1 12 12 ARG N N 15 120.177 0.05 . . . . . . . 383 ARG N . 51068 1 56 . 1 . 1 18 18 GLY C C 13 171.457 0.01 . . . . . . . 389 GLY C . 51068 1 57 . 1 . 1 18 18 GLY CA C 13 42.154 0.14 . . . . . . . 389 GLY CA . 51068 1 58 . 1 . 1 19 19 GLY H H 1 8.045 0.00 . . . . . . . 390 GLY HN . 51068 1 59 . 1 . 1 19 19 GLY C C 13 175.748 0.00 . . . . . . . 390 GLY C . 51068 1 60 . 1 . 1 19 19 GLY CA C 13 41.863 0.00 . . . . . . . 390 GLY CA . 51068 1 61 . 1 . 1 19 19 GLY N N 15 108.581 0.01 . . . . . . . 390 GLY N . 51068 1 62 . 1 . 1 20 20 PRO C C 13 174.692 0.00 . . . . . . . 391 PRO C . 51068 1 63 . 1 . 1 20 20 PRO CA C 13 60.609 0.05 . . . . . . . 391 PRO CA . 51068 1 64 . 1 . 1 20 20 PRO CB C 13 29.350 0.01 . . . . . . . 391 PRO CB . 51068 1 65 . 1 . 1 21 21 MET H H 1 8.449 0.00 . . . . . . . 392 MET HN . 51068 1 66 . 1 . 1 21 21 MET C C 13 174.110 0.01 . . . . . . . 392 MET C . 51068 1 67 . 1 . 1 21 21 MET CA C 13 52.816 0.02 . . . . . . . 392 MET CA . 51068 1 68 . 1 . 1 21 21 MET CB C 13 30.075 0.02 . . . . . . . 392 MET CB . 51068 1 69 . 1 . 1 21 21 MET N N 15 119.983 0.01 . . . . . . . 392 MET N . 51068 1 70 . 1 . 1 22 22 GLY H H 1 8.211 0.00 . . . . . . . 393 GLY HN . 51068 1 71 . 1 . 1 22 22 GLY C C 13 171.483 0.00 . . . . . . . 393 GLY C . 51068 1 72 . 1 . 1 22 22 GLY CA C 13 42.663 0.00 . . . . . . . 393 GLY CA . 51068 1 73 . 1 . 1 22 22 GLY N N 15 109.619 0.01 . . . . . . . 393 GLY N . 51068 1 74 . 1 . 1 24 24 GLY C C 13 171.746 0.01 . . . . . . . 395 GLY C . 51068 1 75 . 1 . 1 24 24 GLY CA C 13 42.452 0.06 . . . . . . . 395 GLY CA . 51068 1 76 . 1 . 1 25 25 GLY H H 1 8.108 0.00 . . . . . . . 396 GLY HN . 51068 1 77 . 1 . 1 25 25 GLY C C 13 171.272 0.00 . . . . . . . 396 GLY C . 51068 1 78 . 1 . 1 25 25 GLY CA C 13 42.358 0.03 . . . . . . . 396 GLY CA . 51068 1 79 . 1 . 1 25 25 GLY N N 15 108.300 0.01 . . . . . . . 396 GLY N . 51068 1 80 . 1 . 1 26 26 TYR H H 1 8.090 0.00 . . . . . . . 397 TYR HN . 51068 1 81 . 1 . 1 26 26 TYR C C 13 173.890 0.01 . . . . . . . 397 TYR C . 51068 1 82 . 1 . 1 26 26 TYR CA C 13 55.423 0.04 . . . . . . . 397 TYR CA . 51068 1 83 . 1 . 1 26 26 TYR CB C 13 36.048 0.01 . . . . . . . 397 TYR CB . 51068 1 84 . 1 . 1 26 26 TYR N N 15 120.138 0.00 . . . . . . . 397 TYR N . 51068 1 85 . 1 . 1 27 27 GLY H H 1 8.363 0.00 . . . . . . . 398 GLY HN . 51068 1 86 . 1 . 1 27 27 GLY C C 13 171.935 0.03 . . . . . . . 398 GLY C . 51068 1 87 . 1 . 1 27 27 GLY CA C 13 42.597 0.05 . . . . . . . 398 GLY CA . 51068 1 88 . 1 . 1 27 27 GLY N N 15 111.276 0.01 . . . . . . . 398 GLY N . 51068 1 89 . 1 . 1 28 28 GLY H H 1 7.852 0.00 . . . . . . . 399 GLY HN . 51068 1 90 . 1 . 1 28 28 GLY C C 13 172.031 0.00 . . . . . . . 399 GLY C . 51068 1 91 . 1 . 1 28 28 GLY CA C 13 42.581 0.00 . . . . . . . 399 GLY CA . 51068 1 92 . 1 . 1 28 28 GLY N N 15 108.228 0.00 . . . . . . . 399 GLY N . 51068 1 93 . 1 . 1 30 30 GLY C C 13 171.761 0.00 . . . . . . . 401 GLY C . 51068 1 94 . 1 . 1 30 30 GLY CA C 13 42.453 0.04 . . . . . . . 401 GLY CA . 51068 1 95 . 1 . 1 31 31 SER H H 1 8.269 0.00 . . . . . . . 402 SER HN . 51068 1 96 . 1 . 1 31 31 SER C C 13 172.563 0.01 . . . . . . . 402 SER C . 51068 1 97 . 1 . 1 31 31 SER CA C 13 55.832 0.05 . . . . . . . 402 SER CA . 51068 1 98 . 1 . 1 31 31 SER CB C 13 61.155 0.00 . . . . . . . 402 SER CB . 51068 1 99 . 1 . 1 31 31 SER N N 15 115.586 0.00 . . . . . . . 402 SER N . 51068 1 100 . 1 . 1 32 32 GLY H H 1 8.426 0.00 . . . . . . . 403 GLY HN . 51068 1 101 . 1 . 1 32 32 GLY C C 13 172.057 0.04 . . . . . . . 403 GLY C . 51068 1 102 . 1 . 1 32 32 GLY CA C 13 42.663 0.04 . . . . . . . 403 GLY CA . 51068 1 103 . 1 . 1 32 32 GLY N N 15 110.739 0.01 . . . . . . . 403 GLY N . 51068 1 104 . 1 . 1 33 33 GLY H H 1 8.207 0.00 . . . . . . . 404 GLY HN . 51068 1 105 . 1 . 1 33 33 GLY C C 13 172.166 0.00 . . . . . . . 404 GLY C . 51068 1 106 . 1 . 1 33 33 GLY CA C 13 42.644 0.00 . . . . . . . 404 GLY CA . 51068 1 107 . 1 . 1 33 33 GLY N N 15 108.606 0.01 . . . . . . . 404 GLY N . 51068 1 108 . 1 . 1 37 37 GLY C C 13 171.652 0.00 . . . . . . . 408 GLY C . 51068 1 109 . 1 . 1 37 37 GLY CA C 13 42.348 0.17 . . . . . . . 408 GLY CA . 51068 1 110 . 1 . 1 38 38 GLY H H 1 8.067 0.00 . . . . . . . 409 GLY HN . 51068 1 111 . 1 . 1 38 38 GLY C C 13 170.763 0.00 . . . . . . . 409 GLY C . 51068 1 112 . 1 . 1 38 38 GLY CA C 13 42.161 0.01 . . . . . . . 409 GLY CA . 51068 1 113 . 1 . 1 38 38 GLY N N 15 108.183 0.00 . . . . . . . 409 GLY N . 51068 1 114 . 1 . 1 39 39 PHE H H 1 8.010 0.00 . . . . . . . 410 PHE HN . 51068 1 115 . 1 . 1 39 39 PHE C C 13 171.426 0.00 . . . . . . . 410 PHE C . 51068 1 116 . 1 . 1 39 39 PHE CA C 13 53.117 0.00 . . . . . . . 410 PHE CA . 51068 1 117 . 1 . 1 39 39 PHE CB C 13 36.309 0.00 . . . . . . . 410 PHE CB . 51068 1 118 . 1 . 1 39 39 PHE N N 15 120.582 0.00 . . . . . . . 410 PHE N . 51068 1 119 . 1 . 1 40 40 PRO C C 13 174.304 0.01 . . . . . . . 411 PRO C . 51068 1 120 . 1 . 1 40 40 PRO CA C 13 60.607 0.05 . . . . . . . 411 PRO CA . 51068 1 121 . 1 . 1 40 40 PRO CB C 13 29.202 0.01 . . . . . . . 411 PRO CB . 51068 1 122 . 1 . 1 41 41 SER H H 1 8.352 0.00 . . . . . . . 412 SER HN . 51068 1 123 . 1 . 1 41 41 SER C C 13 172.576 0.01 . . . . . . . 412 SER C . 51068 1 124 . 1 . 1 41 41 SER CA C 13 55.880 0.06 . . . . . . . 412 SER CA . 51068 1 125 . 1 . 1 41 41 SER CB C 13 61.106 0.01 . . . . . . . 412 SER CB . 51068 1 126 . 1 . 1 41 41 SER N N 15 116.149 0.02 . . . . . . . 412 SER N . 51068 1 127 . 1 . 1 42 42 GLY H H 1 8.393 0.00 . . . . . . . 413 GLY HN . 51068 1 128 . 1 . 1 42 42 GLY C C 13 172.041 0.00 . . . . . . . 413 GLY C . 51068 1 129 . 1 . 1 42 42 GLY CA C 13 42.709 0.00 . . . . . . . 413 GLY CA . 51068 1 130 . 1 . 1 42 42 GLY N N 15 110.824 0.01 . . . . . . . 413 GLY N . 51068 1 131 . 1 . 1 48 48 GLY C C 13 171.472 0.00 . . . . . . . 419 GLY C . 51068 1 132 . 1 . 1 48 48 GLY CA C 13 42.444 0.04 . . . . . . . 419 GLY CA . 51068 1 133 . 1 . 1 49 49 GLN H H 1 8.191 0.00 . . . . . . . 420 GLN HN . 51068 1 134 . 1 . 1 49 49 GLN C C 13 173.157 0.01 . . . . . . . 420 GLN C . 51068 1 135 . 1 . 1 49 49 GLN CA C 13 53.133 0.05 . . . . . . . 420 GLN CA . 51068 1 136 . 1 . 1 49 49 GLN CB C 13 26.713 0.03 . . . . . . . 420 GLN CB . 51068 1 137 . 1 . 1 49 49 GLN N N 15 119.632 0.01 . . . . . . . 420 GLN N . 51068 1 138 . 1 . 1 50 50 GLN H H 1 8.377 0.00 . . . . . . . 421 GLN HN . 51068 1 139 . 1 . 1 50 50 GLN C C 13 172.253 0.00 . . . . . . . 421 GLN C . 51068 1 140 . 1 . 1 50 50 GLN CA C 13 53.335 0.07 . . . . . . . 421 GLN CA . 51068 1 141 . 1 . 1 50 50 GLN CB C 13 26.658 0.01 . . . . . . . 421 GLN CB . 51068 1 142 . 1 . 1 50 50 GLN N N 15 122.285 0.00 . . . . . . . 421 GLN N . 51068 1 143 . 1 . 1 51 51 ARG H H 1 7.965 0.00 . . . . . . . 422 ARG HN . 51068 1 144 . 1 . 1 51 51 ARG C C 13 171.640 0.00 . . . . . . . 422 ARG C . 51068 1 145 . 1 . 1 51 51 ARG CA C 13 54.762 0.00 . . . . . . . 422 ARG CA . 51068 1 146 . 1 . 1 51 51 ARG CB C 13 28.749 0.00 . . . . . . . 422 ARG CB . 51068 1 147 . 1 . 1 51 51 ARG N N 15 127.992 0.01 . . . . . . . 422 ARG N . 51068 1 stop_ save_