BMRB Entry 5088
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5088
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Citation: Karan, Elizabeth; Russell, Brandy; Bren, Kara. "Characterization of Hydrogenobacter thermophilus Cytochromes c(552) Expressed in
the Cytoplasm and Periplasm of Escherichia coli" J. Biol. Inorg. Chem. 7, 260-272 (2002).
PubMed: 11935350
Assembly members:
cytochrome c-552, polymer, 81 residues, 9187 Da.
HEC, non-polymer, 618.503 Da.
Natural source: Common Name: not available Taxonomy ID: 940 Superkingdom: Bacteria Kingdom: not available Genus/species: Hydrogenobacter thermophilus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
cytochrome c-552: MNEQLAKQKGCMACHDLKAK
KVGPAYADVAKKYAGRKDAV
DYLAGKIKKGGSGVWGSVPM
PPQNVTDAEAKQLAQWILSI
K
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 406 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ht c-cyt c-552 | 1 |
| 2 | heme | 2 |
Entities:
Entity 1, Ht c-cyt c-552 81 residues - 9187 Da.
| 1 | MET | ASN | GLU | GLN | LEU | ALA | LYS | GLN | LYS | GLY | ||||
| 2 | CYS | MET | ALA | CYS | HIS | ASP | LEU | LYS | ALA | LYS | ||||
| 3 | LYS | VAL | GLY | PRO | ALA | TYR | ALA | ASP | VAL | ALA | ||||
| 4 | LYS | LYS | TYR | ALA | GLY | ARG | LYS | ASP | ALA | VAL | ||||
| 5 | ASP | TYR | LEU | ALA | GLY | LYS | ILE | LYS | LYS | GLY | ||||
| 6 | GLY | SER | GLY | VAL | TRP | GLY | SER | VAL | PRO | MET | ||||
| 7 | PRO | PRO | GLN | ASN | VAL | THR | ASP | ALA | GLU | ALA | ||||
| 8 | LYS | GLN | LEU | ALA | GLN | TRP | ILE | LEU | SER | ILE | ||||
| 9 | LYS |
Entity 2, heme - C34 H34 Fe N4 O4 - 618.503 Da.
| 1 | HEC |
Samples:
sample_1: cytochrome c-5522 – 3.5 mM; heme2 – 3.5 mM; sodium acetate, [U-2H], 120 mM; sodium dithionite mM
condition_1: pH: 5 na; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H TOCSY | sample_1 | not available | condition_1 |
| 1H NOESY | sample_1 | not available | condition_1 |
Software:
FELIX v97 -
NMR spectrometers:
- Varian INOVA 500 MHz