BMRB Entry 10134

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR10134

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Title:
1H and 15N assignment of oxidized Hydrogenobacter thermophilus cytochrome c552
Deposition date:
2007-04-15
Original release date:
2008-02-11
Authors:
Takayama, Shin-ichi; Takahashi, Yo-ta; Mikami, Shin-ichi; Irie, Kiyofumi; Kawano, Shin; Yamamoto, Yasuhiko; Hemmi, Hikaru; Kitahara, Ryo; Yokoyama, Shigeyuki; Akasaka, Kazuyuki
Citation:

Citation: Takayama, Shin-ichi; Takahashi, Yo-ta; Mikami, Shin-ichi; Irie, Kiyofumi; Kawano, Shin; Yamamoto, Yasuhiko; Hemmi, Hikaru; Kitahara, Ryo; Yokoyama, Shigeyuki; Akasaka, Kazuyuki. "Local conformational transition of Hydrogenobacter thermophilus cytochrome c552 relevant to its redox potential."  Biochemistry 46, 9215-9224 (2007).
PubMed: 17658890

Assembly members:

Assembly members:
cytochrome c552, polymer, 80 residues, Formula weight is not available
HEC, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: Hydrogenobacter thermophilus   Taxonomy ID: 940   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Hydrogenobacter thermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pKK223-3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cytochrome c552: NEQLAKQKGCMACHDLKAKK VGPAYADVAKKYAGRKDAVD YLAGKIKKGGSGVWGSVPMP PQNVTDAEAKQLAQWILSIK

Data sets:
Data typeCount
15N chemical shifts73
1H chemical shifts73

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytochrome c1
2oxidized heme2

Entities:

Entity 1, cytochrome c 80 residues - Formula weight is not available

1   ASNGLUGLNLEUALALYSGLNLYSGLYCYS
2   METALACYSHISASPLEULYSALALYSLYS
3   VALGLYPROALATYRALAASPVALALALYS
4   LYSTYRALAGLYARGLYSASPALAVALASP
5   TYRLEUALAGLYLYSILELYSLYSGLYGLY
6   SERGLYVALTRPGLYSERVALPROMETPRO
7   PROGLNASNVALTHRASPALAGLUALALYS
8   GLNLEUALAGLNTRPILELEUSERILELYS

Entity 2, oxidized heme - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_1: cytochrome c552, [U-15N], 1 mM; potassium phosphate 20 mM; potassium ferricyanide 10 mM

Cond_1: pH: 6.0; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availableCond_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 10135 17053 25389 25390
PDB
DBJ BAI69446
EMBL CAA40902
GB ADO45379
REF WP_012963626
SP P15452
AlphaFold P15452

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks