BMRB Entry 5076

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5076

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance Assignment of the unfolded states of Cold Shock Domain of the human YB-1 protein

Deposition date:

2001-07-10

Original release date:

2002-11-19

Authors:

Kloks, Cathelijne; Hoffmann, Astrid; Omichinski, James; Vuister, Geerten; Hilbers, Cornelis; Grzesiek, Stephan

Citation:

Citation: Kloks, Cathelijne; Hoffmann, Astrid; Omichinski, James; Vuister, Geerten; Hilbers, Cornelis; Grzesiek, Stephan. "Resonance Assignment and Secondary Structure of the Cold Shock Domain of the Human YB-1 Protein" J. Biomol. NMR 12, 463-464 (1998).
PubMed: 9835056

Assembly members:

Assembly members:
Cold Shock domain, polymer, 79 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cold Shock domain: MKKVIATKVLGTVKWFNVRN GYGFINRNDTKEDVFVHQTA IKKNNPRKYLRSVGDGETVE FDVVEGEKGAEAANVTGPG

Data sets:

assigned_chemical_shifts
coupling_constants
heteronucl_T1_relaxation
heteronucl_NOEs
- heteronuclear_NOE_500_major_unfolded_state
- heteronuclear_NOE_500_minor_unfolded_state

Data type	Count
1H chemical shifts	121
13C chemical shifts	60
15N chemical shifts	42
coupling constants	17
T1 relaxation values	63

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Cold Shock domain	1

Entities:

Entity 1, Cold Shock domain 79 residues - Formula weight is not available

1

MET

LYS

VAL

ILE

ALA

THR

LYS

VAL

LEU

2

GLY

THR

VAL

LYS

TRP

PHE

ASN

VAL

ARG

ASN

3

GLY

TYR

GLY

PHE

ILE

ASN

ARG

ASN

ASP

THR

4

LYS

GLU

ASP

VAL

PHE

VAL

HIS

GLN

THR

ALA

5

ILE

LYS

ASN

PRO

ARG

LYS

TYR

LEU

6

ARG

SER

VAL

GLY

ASP

GLY

GLU

THR

VAL

GLU

7

PHE

ASP

VAL

GLU

GLY

GLU

LYS

GLY

ALA

8

GLU

ALA

ASN

VAL

THR

GLY

PRO

GLY

Samples:

sample1: Cold Shock domain, [U-15N], 1.5 mM; H2O 90%; D2O 10%

sample2: Cold Shock domain, [U-15N; U-13C], 1.5 mM; H2O 90%; D2O 10%

sample3: Cold Shock domain, [U-15N; U-13C], 1.5 mM; D2O 100%

sample_conditions_one: pH: 6.7; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	not available	not available	sample_conditions_one

Software:

nmrPipe -

NMR spectrometers:

Bruker DRX 600 MHz
Varian Inova 750 MHz
Varian Inova 500 MHz

BMRB	17225 4147
PDB	1H95
DBJ	BAA02569 BAA03376 BAA05380 BAA19849 BAC45236
EMBL	CAA40847 CAA42778 CAA51261 CAA64631 CAB46826
GB	AAA02573 AAA20871 AAA21677 AAA30497 AAA35749
PRF	2210343A
REF	NP_001003127 NP_001016677 NP_001076254 NP_001079367 NP_001080330
SP	P16989 P21573 P62960 P62961 P67808
TPG	DAA29365 DAA30957
AlphaFold	P16989 P21573 P62960 P62961 P67808