BMRB Entry 4147

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PDB ID: 1h95
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4147
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance Assignment and Secondary Structure of the Cold Shock Domain of the Human YB-1 Protein

Deposition date:

1998-06-04

Original release date:

1999-02-07

Authors:

Kloks, Cathelijne; Hoffmann, Astrid; Omichinski, James; Vuister, Geerten; Hilbers, Cornelis; Grzesiek, Stephan

Citation:

Citation: Kloks, Cathelijne; Hoffmann, Astrid; Omichinski, James; Vuister, Geerten; Hilbers, Cornelis; Grzesiek, Stephan. "Resonance Assignment and Secondary Structure of the Cold Shock Domain of the Human YB-1 Protein" J. Biomol. NMR 12, 463-464 (1998).

Assembly members:

Assembly members:
Cold Shock domain, polymer, 79 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cold Shock domain: MKKVIATKVLGTVKWFNVRN GYGFINRNDTKEDVFVHQTA IKKNNPRKYLRSVGDGETVE FDVVEGEKGAEAANVTGPG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_one
coupling_constants
- coupling_constants_one

Data type	Count
13C chemical shifts	285
15N chemical shifts	71
1H chemical shifts	447
coupling constants	70

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	YB1-CSD	1

Entities:

Entity 1, YB1-CSD 79 residues - Formula weight is not available

1

MET

LYS

VAL

ILE

ALA

THR

LYS

VAL

LEU

2

GLY

THR

VAL

LYS

TRP

PHE

ASN

VAL

ARG

ASN

3

GLY

TYR

GLY

PHE

ILE

ASN

ARG

ASN

ASP

THR

4

LYS

GLU

ASP

VAL

PHE

VAL

HIS

GLN

THR

ALA

5

ILE

LYS

ASN

PRO

ARG

LYS

TYR

LEU

6

ARG

SER

VAL

GLY

ASP

GLY

GLU

THR

VAL

GLU

7

PHE

ASP

VAL

GLU

GLY

GLU

LYS

GLY

ALA

8

GLU

ALA

ASN

VAL

THR

GLY

PRO

GLY

Samples:

sample_one: Cold Shock domain, [U-15N], 1.5 mM; H2O 90%; D2O 10%

sample_two: Cold Shock domain, [U-15N;U-13C], 1.5 mM; H2O 90%; D2O 10%

sample_three: Cold Shock domain, [U-15N;U-13C], 1.5 mM; D2O 100%

sample_conditions_one: pH: 6.7; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	not available	not available	sample_conditions_one

Software:

No software information available

NMR spectrometers:

Bruker DRX 600 MHz

BMRB	17225
PDB	1H95
DBJ	BAA02569 BAA03376 BAA05380 BAA19849 BAC45236
EMBL	CAA40847 CAA42778 CAA51261 CAA64631 CAB46826
GB	AAA02573 AAA20871 AAA21677 AAA30497 AAA35749
PRF	2210343A
REF	NP_001003127 NP_001016677 NP_001076254 NP_001079367 NP_001080330
SP	P16989 P21573 P62960 P62961 P67808
TPG	DAA29365 DAA30957
AlphaFold	P16989 P21573 P62960 P62961 P67808