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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5059
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Yee, Adelinda; Chang, X.; Pineda_Lucena, A.; Wu, B.; Semesi, A.; Le, B.; Ramelot, T.; Lee, G.; Bhattacharyya, S.; Gutierrez, P.; Denisov, A.; Lee, C.; Cort, J.; Kozlov, G.; Liao, J.; Finak, G.; Chen, L.; Wishart, D.; Lee, W.; McIntosh, L.; Gehring, Kalle; Kennedy, M.; Edwards, A.; Arrowsmith, Cheryl. "An NMR Approach to Stuctural Proteomics" Proc. Natl. Acad. Sci. U.S.A. 99, 1825-1830 (2002).
PubMed: 11854485
Assembly members:
EC005, polymer, 97 residues, 10802.3 Da.
Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
EC005: MGSSHHHHHHSSGLVPRGSH
MKNIVPDYRLDMVGEPCPYP
AVATLEAMPQLKKGEILEVV
SDCPQSINNIPLDARNHGYT
VLDIQQDGPTIRYLIQK
Data type | Count |
1H chemical shifts | 350 |
13C chemical shifts | 126 |
15N chemical shifts | 59 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | EC005 | 1 |
Entity 1, EC005 97 residues - 10802.3 Da.
1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
3 | MET | LYS | ASN | ILE | VAL | PRO | ASP | TYR | ARG | LEU | ||||
4 | ASP | MET | VAL | GLY | GLU | PRO | CYS | PRO | TYR | PRO | ||||
5 | ALA | VAL | ALA | THR | LEU | GLU | ALA | MET | PRO | GLN | ||||
6 | LEU | LYS | LYS | GLY | GLU | ILE | LEU | GLU | VAL | VAL | ||||
7 | SER | ASP | CYS | PRO | GLN | SER | ILE | ASN | ASN | ILE | ||||
8 | PRO | LEU | ASP | ALA | ARG | ASN | HIS | GLY | TYR | THR | ||||
9 | VAL | LEU | ASP | ILE | GLN | GLN | ASP | GLY | PRO | THR | ||||
10 | ILE | ARG | TYR | LEU | ILE | GLN | LYS |
sample_1: EC005, [U-15N], 2.0 mM; phosphate buffer 50 mM; NaCl 0.15 M; DTT 1 mM; NaN3 1 mM
sample_2: EC005, [U-15N; U-13C], 2.0 mM; phosphate buffer 50 mM; NaCl 0.15 M; DTT 1 mM; NaN3 1 mM
sample_3: EC005 2.0 mM; phosphate buffer 50 mM; NaCl 0.15 M; DTT 1 mM; NaN3 1 mM
Ex-cond_1: pH: 7.5; temperature: 310 K; ionic strength: 0.3 M
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1H-15N TOCSY | not available | not available | Ex-cond_1 |
1H-15N NOESY | not available | not available | Ex-cond_1 |
1H-13C TOCSY | not available | not available | Ex-cond_1 |
1H-13C NOESY | not available | not available | Ex-cond_1 |
HNCACB | not available | not available | Ex-cond_1 |
CBCA(CO)NH | not available | not available | Ex-cond_1 |
XWINNMR v2.1 - collection of data
GIFA v4.31 - data processing
XEASY v1.3.13 - data analysis
PDB | |
DBJ | BAA15758 BAB36092 BAG77685 BAI26061 BAI30978 |
EMBL | CAD05714 CAP76412 CAQ88691 CAQ98867 CAR03284 |
GB | AAA71973 AAA82578 AAC74997 AAG56945 AAL20878 |
PIR | AG0751 |
REF | NP_288391 NP_310696 NP_416440 NP_456527 NP_460919 |
SP | P0AA31 P0AA32 P0AA33 P0AA34 P0AA35 |
AlphaFold | P0AA31 P0AA32 P0AA33 P0AA34 P0AA35 |
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