The His-tag was not cut off for the NMR study, even though the chemical shifts were not reported here.

Proceedings of the National Academy of Sciences of the United States of America
An NMR approach to structural proteomics.

The 465,813 base pair sequence corresponding to the 40.1-50.0 min region on the genetic map of Escherichia coli K-12 (W3110) was determined. Analysis of the sequence revealed that this region contained at least 466 potential open reading frames, of which 187 (40%) were previously reported, 105 (23%) were homologous to other known genes, 103 (22%) were identical or similar to hypothetical genes registered in databases, and the remaining 71 (15%) did not show a significant similarity to any other gene. At the 45.2-46.0 min region, we found a very large cluster of about 30 genes, whose functions are involved in the biosynthesis of polysaccharides as the components of outer membranes. In addition, we identified a new asn-tRNA gene, designated asnW, between the asnT and asnU genes and a new lysogenic phage attachment site as the cis-element.

Itoh T, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Kasai H,
Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T,
Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S,
Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N,
Sampei G, Seki Y, Horiuchi T, et al.
A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the
40.1-50.0 min region on the linkage map.
DNA Res. 1996 Dec 31;3(6):379-92.

DNA research : an international journal for rapid publication of reports on genes and genomes
A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.

Solution Structure Of Ec005 From Escherichia Coli

conserved hypothetical protein [Escherichia coli str. K-12 substr. W3110]

hypothetical protein [Escherichia coli O157:H7 str. Sakai]

conserved hypothetical protein [Escherichia coli SE11]

conserved predicted protein [Escherichia coli O26:H11 str. 11368]

conserved predicted protein [Escherichia coli O103:H2 str. 12009]

conserved hypothetical protein [Salmonella enterica subsp. enterica serovar Typhi str. CT18]

UPF0033 protein yedF [Escherichia coli LF82]

conserved hypothetical protein [Escherichia fergusonii ATCC 35469]

conserved hypothetical protein [Escherichia coli IAI1]

conserved hypothetical protein [Escherichia coli S88]

level of amino acid identity between E. coli and S.typhimurium proteins strongly indicates authentic gene [Salmonella enterica

putative TusA family sulfurtransferase [Escherichia coli str. K-12 substr. MG1655]

orf, hypothetical protein [Escherichia coli O157:H7 str. EDL933]

putative transcriptional regulator [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]

conserved hypothetical protein STY2174 [imported] - Salmonella enterica subsp. enterica serovar Typhi (strain CT18)

hypothetical protein Z3020 [Escherichia coli O157:H7 str. EDL933]

hypothetical protein ECs2669 [Escherichia coli O157:H7 str. Sakai]

hypothetical protein STY2174 [Salmonella enterica subsp. enterica serovar Typhi str. CT18]

hypothetical protein STM1966 [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]

RecName: Full=UPF0033 protein YedF [Escherichia coli K-12]

RecName: Full=UPF0033 protein YedF [Escherichia coli CFT073]

RecName: Full=UPF0033 protein YedF [Escherichia coli O157:H7]

RecName: Full=UPF0033 protein YedF [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]

RecName: Full=UPF0033 protein YedF [Salmonella enterica subsp. enterica serovar Typhi]

Residues 1-20 are from the His-Tag. EC005 actually comprises resiudes 20-97 in this deposition.
Chemical shift assignments for EC005 from E. coli