BMRB Entry 5025

Title:
The Solution Structure of the Complex Formed between alpha-Bungarotoxin and an 18mer Cognate Peptide Derived from the alpha1 Subunit of the Nicotinic Acetylcholine Receptor from Torpedo californica
Deposition date:
2001-05-21
Original release date:
2001-07-16
Authors:
Zeng, Haoyu; Moise, Leonard; Grant, Marianne; Hawrot, Edward
Citation:

Citation: Zeng, Haoyu; Moise, Leonard; Grant, Marianne; Hawrot, Edward. "The Solution Structure of the Complex Formed between alpha-Bungarotoxin and an 18mer Cognate Peptide Derived from the alpha1 Subunit of the Nicotinic Acetylcholine Receptor from Torpedo californica"  J. Biol. Chem. 276, 22930-22940 (2001).
PubMed: 11312275

Assembly members:

Assembly members:
alpha-neurotoxin, polymer, 74 residues, 7977 Da.
18mer, polymer, 19 residues, 2338 Da.

Natural source:

Natural source:   Common Name: Many-banded krait   Taxonomy ID: 8616   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bungarus multicinctus

Experimental source:

Experimental source:   Production method: vendor

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts355
15N chemical shifts15

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ALPHA-BUNGAROTOXIN1
218mer2

Entities:

Entity 1, ALPHA-BUNGAROTOXIN 74 residues - 7977 Da.

1   ILEVALCYSHISTHRTHRALATHRSERPRO
2   ILESERALAVALTHRCYSPROPROGLYGLU
3   ASNLEUCYSTYRARGLYSMETTRPCYSASP
4   ALAPHECYSSERSERARGGLYLYSVALVAL
5   GLULEUGLYCYSALAALATHRCYSPROSER
6   LYSLYSPROTYRGLUGLUVALTHRCYSCYS
7   SERTHRASPLYSCYSASNPROHISPROLYS
8   GLNARGPROGLY

Entity 2, 18mer 19 residues - 2338 Da.

1   TYRARGGLYTRPLYSHISTRPVALTYRTYR
2   THRCYSCYSPROASPTHRPROTYRHSL

Samples:

sample_1: alpha-neurotoxin 2.1 mM; 18mer, [U-99% 15N], 2.1 mM; potassium acetate buffer, [U-2H], 50 mM; H2O 95%; D2O 5%; sodium azide 0.05%

sample_cond_1: pH: 4.0; temperature: 308 K; ionic strength: 29 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated TOCSYsample_1not availablesample_cond_1
3D HNHAsample_1not availablesample_cond_1
3D 15N-separated NOESYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
2D NOESYsample_1not availablesample_cond_1
2D 1H-15N HSQCsample_1not availablesample_cond_1

Software:

XWINNMR v2.1 - collection

NMRPipe v1.8 - processing

Sparky v3.95 - data analysis

CNSsolve v1.0 - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

SWISS-PROT P02711 P02710
PRF 0811252A
PIR A39718
GenBank ABS70800 AAR29368 AAR29364 AAA96705 AAA96704
PDB
BMRB 4838