BMRB Entry 4838
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4838
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Samson, Abraham; Chill, Jordan; Rodriguez, Erik; Scherf, Tali; Anglister, Jacob. "NMR Mapping and Secondary Structure Determination of the Major Acetylcholine
Receptor alpha-Subunit Determinant Interacting with alpha-Bungarotoxin" Biochemistry 40, 5464-5473 (2001).
PubMed: 11331011
Assembly members:
alpha-bungarotoxin, polymer, 74 residues, Formula weight is not available
acetylcholine receptor peptide, polymer, 25 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 8616 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bungarus multicinctus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
alpha-bungarotoxin: IVCHTTATSPISAVTCPPGE
NLCYRKMWCDAFCSSRGKVV
ELGCAATCPSKKPYEEVTCC
STDKCNPHPKRQPG
acetylcholine receptor peptide: EERGWKHWVYYTCCPDTPYL
DITEE
| Data type | Count |
| 1H chemical shifts | 477 |
| coupling constants | 79 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | a-bungarotoxin | 1 |
| 2 | Acetylcholine receptor peptide | 2 |
Entities:
Entity 1, a-bungarotoxin 74 residues - Formula weight is not available
| 1 | ILE | VAL | CYS | HIS | THR | THR | ALA | THR | SER | PRO | ||||
| 2 | ILE | SER | ALA | VAL | THR | CYS | PRO | PRO | GLY | GLU | ||||
| 3 | ASN | LEU | CYS | TYR | ARG | LYS | MET | TRP | CYS | ASP | ||||
| 4 | ALA | PHE | CYS | SER | SER | ARG | GLY | LYS | VAL | VAL | ||||
| 5 | GLU | LEU | GLY | CYS | ALA | ALA | THR | CYS | PRO | SER | ||||
| 6 | LYS | LYS | PRO | TYR | GLU | GLU | VAL | THR | CYS | CYS | ||||
| 7 | SER | THR | ASP | LYS | CYS | ASN | PRO | HIS | PRO | LYS | ||||
| 8 | ARG | GLN | PRO | GLY |
Entity 2, Acetylcholine receptor peptide 25 residues - Formula weight is not available
| 1 | GLU | GLU | ARG | GLY | TRP | LYS | HIS | TRP | VAL | TYR | ||||
| 2 | TYR | THR | CYS | CYS | PRO | ASP | THR | PRO | TYR | LEU | ||||
| 3 | ASP | ILE | THR | GLU | GLU |
Samples:
sample_1: alpha-bungarotoxin 2 mM; acetylcholine receptor peptide 2 mM
ex-cond1: pH: 4; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HOHAHA | not available | not available | not available |
| NOESY | not available | not available | not available |
| DQF-COSY | not available | not available | not available |
Software:
Xwinnmr -
NMR spectrometers:
- Bruker DRX 800 MHz