BMRB Entry 5011
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5011
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Langdon, Grant; Jimenez, M. Angeles; Genzor, Carlos; Maldonado, Susana; Sancho, Javier; Rico, Manuel. "Anabaena apoflavodoxin hydrogen exchange: on the stable exchange core of the
alfa/beta(21345) flavodoxin-like family" Proteins: Struct. Funct. Genet. 43, 476-488 (2001).
Assembly members:
Anabaena apoflavodoxin, polymer, 169 residues, 18886 Da.
Natural source: Common Name: Anabaena PCC7119 Taxonomy ID: 1168 Superkingdom: Eubacteria Kingdom: not available Genus/species: Anabaena PCC7119
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: ptrc99a
Entity Sequences (FASTA):
Anabaena apoflavodoxin: AKKIGLFYGTQTGKTESVAE
IIRDEFGNDVVTLHDVSQAE
VTDLNDYQYLIIGCPTWNIG
ELQSDWEGLYSELDDVDFNG
KLVAYFGTGDQIGYADNFQD
AIGILEEKISQRGGKTVGYW
STDGYDFNDSKALRNGKFVG
LALDEDNQSDLTDDRIKSWV
AQLKSEFGL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 861 |
| 13C chemical shifts | 23 |
| 15N chemical shifts | 156 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | apofld monomer | 1 |
Entities:
Entity 1, apofld monomer 169 residues - 18886 Da.
| 1 | ALA | LYS | LYS | ILE | GLY | LEU | PHE | TYR | GLY | THR | ||||
| 2 | GLN | THR | GLY | LYS | THR | GLU | SER | VAL | ALA | GLU | ||||
| 3 | ILE | ILE | ARG | ASP | GLU | PHE | GLY | ASN | ASP | VAL | ||||
| 4 | VAL | THR | LEU | HIS | ASP | VAL | SER | GLN | ALA | GLU | ||||
| 5 | VAL | THR | ASP | LEU | ASN | ASP | TYR | GLN | TYR | LEU | ||||
| 6 | ILE | ILE | GLY | CYS | PRO | THR | TRP | ASN | ILE | GLY | ||||
| 7 | GLU | LEU | GLN | SER | ASP | TRP | GLU | GLY | LEU | TYR | ||||
| 8 | SER | GLU | LEU | ASP | ASP | VAL | ASP | PHE | ASN | GLY | ||||
| 9 | LYS | LEU | VAL | ALA | TYR | PHE | GLY | THR | GLY | ASP | ||||
| 10 | GLN | ILE | GLY | TYR | ALA | ASP | ASN | PHE | GLN | ASP | ||||
| 11 | ALA | ILE | GLY | ILE | LEU | GLU | GLU | LYS | ILE | SER | ||||
| 12 | GLN | ARG | GLY | GLY | LYS | THR | VAL | GLY | TYR | TRP | ||||
| 13 | SER | THR | ASP | GLY | TYR | ASP | PHE | ASN | ASP | SER | ||||
| 14 | LYS | ALA | LEU | ARG | ASN | GLY | LYS | PHE | VAL | GLY | ||||
| 15 | LEU | ALA | LEU | ASP | GLU | ASP | ASN | GLN | SER | ASP | ||||
| 16 | LEU | THR | ASP | ASP | ARG | ILE | LYS | SER | TRP | VAL | ||||
| 17 | ALA | GLN | LEU | LYS | SER | GLU | PHE | GLY | LEU |
Samples:
sample_1: Anabaena apoflavodoxin 2.0 mM; sodium phosphate 50 mM; dithiothreitol 2 mM
sample_2: Anabaena apoflavodoxin, [U-15N], 2.0 mM; sodium phosphate 50 mM; dithiothreitol 2 mM
sample_3: Anabaena apoflavodoxin 2.0 mM
Ex-cond_1: pH: 7.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H COSY | not available | not available | not available |
| 2D 1H-1H CLEAN-TOCSY | not available | not available | not available |
| 2D 1H-1H NOESY | not available | not available | not available |
| 2D 1H-15N HSQC | not available | not available | not available |
| 3D 1H-1H-15N TOCSY | not available | not available | not available |
| 3D 1H-1H-15N NOESY | not available | not available | not available |
Software:
XEASY -
NMR spectrometers:
- Bruker AMX 600 MHz
- Varian INOVA 750 MHz
Related Database Links:
| BMRB | 1580 16593 |
| PDB | |
| DBJ | BAB74104 |
| EMBL | CAA32720 |
| GB | AAB20462 |
| PIR | S04600 S18374 |
| REF | NP_486445 WP_010996561 |
| SP | P0A3D9 P0A3E0 |
| AlphaFold | P0A3D9 P0A3E0 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks