BMRB Entry 16593

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16593

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Title:
1H, 15N and 13C chemical shifts for the apo form of F98N-flavodoxin from Anabaena PCC7119
Deposition date:
2009-11-02
Original release date:
2010-06-28
Authors:
Ayuso-Tejedor, Sara; Sancho, Javier; Jimenez, M. Angeles
Citation:

Citation: Ayuso-Tejedor, Sara; Angarica, Vladimir Espinosa; Bueno, Marta; Campos, Luis; Abian, Olga; Bernado, Pau; Sancho, Javier; Jimenez, M. Angeles. "Design and structure of an equilibrium protein folding intermediate: a hint into dynamical regions of proteins."  J. Mol. Biol. 400, 922-934 (2010).
PubMed: 20553732

Assembly members:

Assembly members:
F98N-fdx, polymer, 169 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Anabaena PCC 7119   Taxonomy ID: 1168   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Anabaena not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTrc99A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
F98N-fdx: AKKIGLFYGTQTGKTESVAE IIRDEFGNDVVTLHDVSQAE VTDLNDYQYLIIGCPTWNIG ELQSDWEGLYSELDDVDFNG KLVAYFGTGDQIGYADNFQD AIGILEEKISQRGGKTVGYW STDGYDFNDSKALRNGKFVG LALDEDNQSDLTDDRIKSWV AQLKSEFGL

Data sets:
Data typeCount
13C chemical shifts33
15N chemical shifts79
1H chemical shifts601

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1F98N-fdx1

Entities:

Entity 1, F98N-fdx 169 residues - Formula weight is not available

F98N mutant of flavodoxin from Anabaena PC 7119

1   ALALYSLYSILEGLYLEUPHETYRGLYTHR
2   GLNTHRGLYLYSTHRGLUSERVALALAGLU
3   ILEILEARGASPGLUPHEGLYASNASPVAL
4   VALTHRLEUHISASPVALSERGLNALAGLU
5   VALTHRASPLEUASNASPTYRGLNTYRLEU
6   ILEILEGLYCYSPROTHRTRPASNILEGLY
7   GLULEUGLNSERASPTRPGLUGLYLEUTYR
8   SERGLULEUASPASPVALASPPHEASNGLY
9   LYSLEUVALALATYRPHEGLYTHRGLYASP
10   GLNILEGLYTYRALAASPASNPHEGLNASP
11   ALAILEGLYILELEUGLUGLULYSILESER
12   GLNARGGLYGLYLYSTHRVALGLYTYRTRP
13   SERTHRASPGLYTYRASPPHEASNASPSER
14   LYSALALEUARGASNGLYLYSPHEVALGLY
15   LEUALALEUASPGLUASPASNGLNSERASP
16   LEUTHRASPASPARGILELYSSERTRPVAL
17   ALAGLNLEULYSSERGLUPHEGLYLEU

Samples:

sample_1: F98N-fdx 1 mM; sodium phosphate 5 mM; H2O 90%; D2O 10%; DSS 0.1 mM

sample_2: F98N-fdx 1 mM; sodium phosphate 5 mM; D2O 100%; DSS 0.1 mM

sample_3: F98N-fdx, [U-15N], 1 mM; sodium phosphate 5 mM; H2O 90%; D2O 10%; DSS 0.1 mM

sample_conditions_1: ionic strength: 5 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_3isotropicsample_conditions_1
3D HNHAsample_3isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 1580
PDB
DBJ BAB74104
EMBL CAA32720
GB AAB20462
PIR S04600 S18374
REF WP_010996561
SP P0A3D9 P0A3E0
AlphaFold P0A3D9 P0A3E0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks