BMRB Entry 4931

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4931

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution structure of dynein light chain 8 (DLC8) and bim peptide complex
Deposition date:
2000-12-21
Original release date:
2001-08-22
Authors:
Fan, Jing-Song.; Zhang, Qiang; Tochio, Hidehito; Li, Ming; Zhang, Mingjie
Citation:

Citation: Fan, Jing-song.; Zhang, Qiang; Tochio, Hidehito; Li, Ming; Zhang, Mingjie. "Structural Basis of Diverse Sequence-dependent Target Recognition by the 8 kDa Dynein Light Chain "  J. Mol. Biol. 306, 97-108 (2001).
PubMed: 11178896

Assembly members:

Assembly members:
DYNEIN light chain 8, polymer, 89 residues, 10300 Da.
Bcl-2, polymer, 9 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET14B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DYNEIN light chain 8: MCDRKAVIKNADMSEEMQQD SVECATQALEKYNIEKDIAA HIKKEFDKKYNPTWHCIVGR NFGSYVTHETKHFIYFYLGQ VAILLFKSG
Bcl-2: MSCDKSTQT

Data sets:
Data typeCount
1H chemical shifts608
13C chemical shifts259
15N chemical shifts89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dynein unit 11
2dynein unit 21
3Bim unit 12
4Bim unit 22

Entities:

Entity 1, dynein unit 1 89 residues - 10300 Da.

1   METCYSASPARGLYSALAVALILELYSASN
2   ALAASPMETSERGLUGLUMETGLNGLNASP
3   SERVALGLUCYSALATHRGLNALALEUGLU
4   LYSTYRASNILEGLULYSASPILEALAALA
5   HISILELYSLYSGLUPHEASPLYSLYSTYR
6   ASNPROTHRTRPHISCYSILEVALGLYARG
7   ASNPHEGLYSERTYRVALTHRHISGLUTHR
8   LYSHISPHEILETYRPHETYRLEUGLYGLN
9   VALALAILELEULEUPHELYSSERGLY

Entity 2, Bim unit 1 9 residues - Formula weight is not available

1   METSERCYSASPLYSSERTHRGLNTHR

Related Database Links:

SWISS-PROT P63169 P63168 P63167 P61285 P61273
REF NP_003737 NP_001075487 NP_001032584 NP_001032583 NP_001003901
GenBank AAD01643 AAC32531 AAC32530 AAB38257 AAB04149
EMBL CAG46925 CAG28600
DBJ BAB28973 BAB28970 BAB27117 BAB27063 BAB22160
PDB 1F96 1F95 1F3C 1CMI
BMRB 4912 4911 4305

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks