BMRB Entry 4911

Title:
Structural Basis of Diverse Sequence-dependent Target Recognition by the 8kDa Dynein light chain   PubMed: 11178896
Deposition date:
2000-12-06
Original release date:
2001-08-08
Authors:
Fan, Jing-Song; Zhang, Qiang; Tochio, Hidehito; Li, Ming; Zhang, Mingjie
Citation:

Citation: Fan, Jing-Song; Zhang, Qiang; Tochio, Hidehito; Li, Ming; Zhang, Mingjie. "Structural Basis of Diverse Sequence-dependent Target Recognition by the 8kDa Dynein light chain"  J. Mol. Biol. 306, 97-108 (2001).

Assembly members:

Assembly members:
DYNEIN light chain 8, polymer, 89 residues, 10300 Da.
nNOS peptide, polymer, 18 residues, 2000 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: E. coli

Experimental source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: E. coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts632
13C chemical shifts259
15N chemical shifts97

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DYNEIN light chain 8, DLC8, subunit a1
2DYNEIN light chain 8, DLC8, subunit b1
3nNOS fragment peptide, subunit a2
4nNOS fragment peptide, subunit b2

Entities:

Entity 1, DYNEIN light chain 8, DLC8, subunit a 89 residues - 10300 Da.

1   METCYSASPARGLYSALAVALILELYSASN
2   ALAASPMETSERGLUGLUMETGLNGLNASP
3   SERVALGLUCYSALATHRGLNALALEUGLU
4   LYSTYRASNILEGLULYSASPILEALAALA
5   HISILELYSLYSGLUPHEASPLYSLYSTYR
6   ASNPROTHRTRPHISCYSILEVALGLYARG
7   ASNPHEGLYSERTYRVALTHRHISGLUTHR
8   LYSHISPHEILETYRPHETYRLEUGLYGLN
9   VALALAILELEULEUPHELYSSERGLY

Entity 2, nNOS fragment peptide, subunit a 18 residues - 2000 Da.

1   METLYSASPTHRLYSILEGLNVALASPARG
2   ASPLEUASPGLYLYSSERHISLYS

Samples:

sample_1: DYNEIN light chain 8, [U-13C; U-15N], 1.5 mM; nNOS peptide, [U-13C; U-15N], 1.5 mM; phosphate buffer 100 mM; DTT 10 mM; D2O 10%; H2O 90%

sample_cond_1: pH: 7.0; temperature: 303 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
HNCACB and CBCACONNH for backbone assignmentsample_1not availablesample_cond_1
HCCH-TOCSY for side-chain assignmentsample_1not availablesample_cond_1

Software:

VNMR v6.1b - data collection

NMRPipe v1.7 - processing

X-PLOR v3.8 - structure solution

CNS v1.0 - spectra, refinement

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 750 MHz

Related Database Links:

SWISS-PROT P63169 P63168 P63167 P61285 P61273
REF NP_003737 NP_001075487 NP_001032584 NP_001032583 NP_001003901
GenBank AAD01643 AAC32531 AAC32530 AAB38257 AAB04149
EMBL CAG46925 CAG28600
DBJ BAB28973 BAB28970 BAB27117 BAB27063 BAB22160
PDB
BMRB 4931 4912 4305

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts