BMRB Entry 4927

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PDB ID: 1n02
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4927
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, 15N Resonance Assignments of a Circular Permuted Variant of CV-N (cpCV-N)

Deposition date:

2000-12-18

Original release date:

2002-04-04

Authors:

Barrientos, Laura; Campos-Olivas, Ramon; Gronenborn, Angela

Citation:

Citation: Barrientos, Laura; Campos-Olivas, Ramon; Louis, John; Fiser, Andras; Sali, Andrej; Gronenborn, Angela. "Letter to the editor: 1H, 13C, 15N Resonance Assignments and Fold Verification of a Circular Permuted Variant of the Potent HIV-inactivating Protein Cyanovirin-N" J. Biomol. NMR 19, 289-290 (2001).

Assembly members:

Assembly members:
Cyanovirin-N, polymer, 102 residues, 11013 Da.

Natural source:

Natural source: Common Name: Cyanobacterium Taxonomy ID: not available Superkingdom: Eubacteria Kingdom: not available Genus/species: Nostoc ellipsosporum

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cyanovirin-N: GLGKFIETCRNTQLAGSSEL AAECKTRAQQFVSTKINLDD HIANIDGTLKWQPSNFSQTC YNSAIQGSVLTSTCERTNGG YNTSSIDLNSVIENVDGSLK YE

Data sets:

assigned_chemical_shifts
- shift

Data type	Count
13C chemical shifts	423
15N chemical shifts	116
1H chemical shifts	632

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Circular Permuted Cyanovirin-N, cpCV-N monomer	1

Entities:

Entity 1, Circular Permuted Cyanovirin-N, cpCV-N monomer 102 residues - 11013 Da.

1

GLY

LEU

GLY

LYS

PHE

ILE

GLU

THR

CYS

ARG

2

ASN

THR

GLN

LEU

ALA

GLY

SER

GLU

LEU

3

ALA

GLU

CYS

LYS

THR

ARG

ALA

GLN

4

PHE

VAL

SER

THR

LYS

ILE

ASN

LEU

ASP

5

HIS

ILE

ALA

ASN

ILE

ASP

GLY

THR

LEU

LYS

6

TRP

GLN

PRO

SER

ASN

PHE

SER

GLN

THR

CYS

7

TYR

ASN

SER

ALA

ILE

GLN

GLY

SER

VAL

LEU

8

THR

SER

THR

CYS

GLU

ARG

THR

ASN

GLY

9

TYR

ASN

THR

SER

ILE

ASP

LEU

ASN

SER

10

VAL

ILE

GLU

ASN

VAL

ASP

GLY

SER

LEU

LYS

11

TYR

GLU

Samples:

sample_1: Cyanovirin-N, [U-15N], 0.3 – 1 mM; phosphate buffer 20 mM

sample_2: Cyanovirin-N, [U-15N; U-13C], 0.3 – 1.0 mM; phosphate buffer 20 mM

Overall_conditions: pH: 6.0; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	not available	not available	not available
2D 1H-13C HSQC/HMQC	not available	not available	not available
3D HNCO	not available	not available	not available
3D HNCACB	not available	not available	not available
3D CBCA(CO)NH	not available	not available	not available
3D HCCH-TOCSY	not available	not available	not available
3D DIPSI-H(CCO)NH	not available	not available	not available
3D HNHA	not available	not available	not available
3D 15N-edited NOESY	not available	not available	not available
4D 13C,13C NOESY	not available	not available	not available

Software:

NMRView v4.1.1 -

NMR spectrometers:

Bruker DMX 500 MHz
Bruker DMX 600 MHz
Bruker DMX 750 MHz
Bruker DRX 800 MHz