1H is referenced directly by assigning to the detected H2O signal (at 300K) a value of 4.754 ppm. 13C and 15N shifts are referenced indirectly to 1H. The errors in the chemical shift of a particular atom are upper limit estimates from the spectral resolution in the spectrum where the corresponding signal was assigned.

1H, 13C, 15N resonance assignments and fold verification of a circular permuted variant of the potent HIV-inactivating protein cyanovirin-N.

The molecule's sequence is that of a circular permuted variant of Cyanovirin-N. New N- and C-termini were introduced into an exposed helical loop and the original termini were linked using residues of the original loop.

Circular Permuted Cyanovirin-N, cpCV-N monomer

Circular Permuted Variant of Cyanovirin-N

Solution Structure Of A Circular-Permuted Variant Of The Potent Hiv-Inactivating Protein Cyanovirin-N

1H, 13C, 15N Resonance Assignments of a Circular Permuted Variant of CV-N (cpCV-N)

for dyana, Standard target function-simulated annealing protocol. For CNS, Simulated-annealing in Cartesian space