BMRB Entry 4870

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4870

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Title:
Sequence-specific 1H, 15N, and 13C resonance assignments for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit
Deposition date:
2000-10-20
Original release date:
2011-08-10
Authors:
Poznanski, Jaroslaw; Zhukow, Igor; Bolewska, Krystyna; Wierzchowski, Kazimierz
Citation:

Citation: Poznanski, Jaroslaw; Zhukov, Igor; Bolewska, Krystyna; Wierzchowski, Kazimierz. "Letter to the Editor: Sequence-specific 1H, 15N, and 13C resonance assignments for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit"  J. Biomol. NMR 20, 181-182 (2001).

Assembly members:

Assembly members:
region 4 of sigma70 of E. coli RNA polymerase, polymer, 106 residues, 12164 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
region 4 of sigma70 of E. coli RNA polymerase: GSSHHHHHHSSGLVPRGSHM LELPLDSATTESLRAATHDV LAGLTAREAKVLRMRFGIDM NTDYTLEEVGKQFDVTRERI RQIEAKALRKLRHPSRSEVL RSFLDD

Data sets:
Data typeCount
1H chemical shifts681
13C chemical shifts435
15N chemical shifts108
coupling constants66
T1 relaxation values94
T2 relaxation values94

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
14 sigma701

Entities:

Entity 1, 4 sigma70 106 residues - 12164 Da.

1   GLYSERSERHISHISHISHISHISHISSER
2   SERGLYLEUVALPROARGGLYSERHISMET
3   LEUGLULEUPROLEUASPSERALATHRTHR
4   GLUSERLEUARGALAALATHRHISASPVAL
5   LEUALAGLYLEUTHRALAARGGLUALALYS
6   VALLEUARGMETARGPHEGLYILEASPMET
7   ASNTHRASPTYRTHRLEUGLUGLUVALGLY
8   LYSGLNPHEASPVALTHRARGGLUARGILE
9   ARGGLNILEGLUALALYSALALEUARGLYS
10   LEUARGHISPROSERARGSERGLUVALLEU
11   ARGSERPHELEUASPASP

Related Database Links:

BMRB 15930 15936 15975
PDB 1TLH 2P7V 3IYD 3T72 4IGC 4JK1 4JK2 4JKR 4KMU 4KN4 4KN7 4LJZ 4LK0 4LK1 4LLG 4MEX 4MEY
DBJ BAB37373 BAE77118 BAG66772 BAG78873 BAH65247
EMBL CAD07736 CAL34114 CAP77541 CAQ33404 CAQ90501
GB AAA24601 AAA27242 AAA89147 AAB60181 AAC76103
PIR AB0893
REF NP_289642 NP_311977 NP_417539 NP_457602 NP_462126
SP P00579 P0A2E3 P0A2E4
AlphaFold P00579 P0A2E3 P0A2E4

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