BMRB Entry 15936

Name: Sequence-specific 1H, 15N, resonance assignments of nine 1H-{15N} HSQC spectras for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit recorded at TFE concentrations increasing in the range of 0-30%
Published: 2009-11-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15936

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Sequence-specific 1H, 15N, resonance assignments of nine 1H-{15N} HSQC spectras for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit recorded at TFE concentrations increasing in the range of 0-30%

Deposition date:

2008-09-01

Original release date:

2009-11-20

Authors:

Kaczka, Piotr; Polkowska, Agnieszka

Citation:

Citation: Kaczka, Piotr; Polkowska-Nowakowska, Agnieszka; Bolewska, Krystyna; Zhukov, Igor; Poznaski, Jarosaw; Wierzchowski, Kazimierz. "Backbone dynamics of TFE-induced native-like fold of region 4 of Escherichia coli RNA polymerase sigma70 subunit." Proteins 78, 754-768 (2010).
PubMed: 19847776

Assembly members:

Assembly members:
4_sigma70, polymer, 107 residues, 12032.5 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
4_sigma70: MGSSHHHHHHSSGLVPRGSH MLELPLDSATTESLRAATHD VLAGLTAREAKVLRMRFGID MNTDYTLEEVGKQFDVTRER IRQIEAKALRKLRHPSRSEV LRSFLDD

Data sets:

assigned_chemical_shifts

Data type	Count
15N chemical shifts	1000
1H chemical shifts	1000

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	4 sigma70	1

Entities:

Entity 1, 4 sigma70 107 residues - 12032.5 Da.

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

LEU

GLU

LEU

PRO

LEU

ASP

SER

ALA

THR

4

THR

GLU

SER

LEU

ARG

ALA

THR

HIS

ASP

5

VAL

LEU

ALA

GLY

LEU

THR

ALA

ARG

GLU

ALA

6

LYS

VAL

LEU

ARG

MET

ARG

PHE

GLY

ILE

ASP

7

MET

ASN

THR

ASP

TYR

THR

LEU

GLU

VAL

8

GLY

LYS

GLN

PHE

ASP

VAL

THR

ARG

GLU

ARG

9

ILE

ARG

GLN

ILE

GLU

ALA

LYS

ALA

LEU

ARG

10

LYS

LEU

ARG

HIS

PRO

SER

ARG

SER

GLU

VAL

11

LEU

ARG

SER

PHE

LEU

ASP

Samples:

sample_1: 4 sigma70, [U-13C; U-15N], 3 mM

sample_2: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 0.3%

sample_3: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 2%

sample_4: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 4%

sample_5: 4 of sigma70, [U-13C; U-15N], 3 mM; TFE 6%

sample_6: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 10%

sample_7: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 12%

sample_8: 4 sigma70 3 mM; TFE 15%

sample_9: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 20%

sample_10: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 30%

sample_conditions_all: ionic strength: 0.0 M; pH: 4.55; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_all
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_all
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_all
2D 1H-15N HSQC	sample_4	isotropic	sample_conditions_all
2D 1H-15N HSQC	sample_5	isotropic	sample_conditions_all
2D 1H-15N HSQC	sample_6	isotropic	sample_conditions_all
2D 1H-15N HSQC	sample_7	isotropic	sample_conditions_all
2D 1H-15N HSQC	sample_8	isotropic	sample_conditions_all
2D 1H-15N HSQC	sample_9	isotropic	sample_conditions_all
2D 1H-15N HSQC	sample_10	isotropic	sample_conditions_all

Software:

NMRPipe v1.7, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.111, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Varian UnityPlus 500 MHz

BMRB	15930 15975
PDB	1TLH 2P7V 3IYD 3T72 4JK1 4JK2 4JKR 4KMU 4KN4 4KN7 4LJZ 4LK0 4LK1 4LLG 4MEX 4MEY 4XSX 4XSY 4XSZ 4YFK 4YFN 4YFX 4YG2 4YLN 4YLO 4YLP 4ZH2 4ZH3 4ZH4
DBJ	BAB37373 BAE77118 BAG66772 BAG78873 BAH65247
EMBL	CAD07736 CAL34114 CAP77541 CAQ33404 CAQ90501
GB	AAA24601 AAA27242 AAA89147 AAB60181 AAC76103
PIR	AB0893
REF	NP_311977 NP_417539 NP_457602 NP_462126 NP_708877
SP	P00579 P0A2E3 P0A2E4
AlphaFold	P00579 P0A2E3 P0A2E4